Based on the anti-aggregation mechanism of citrate stabilized gold nanoparticles (AuNPs), a new specific and sensitive colorimetric sensor for thiocyanate (SCN-) was developed. In this scheme, the AuNPs were aggregated in the presence of the aggregating agent 2-aminopyridine (2-AP) due to electrostatic attraction. The solution color changed from red to blue. When SCNwas present, SCNformed sulfur-gold bond with the AuNPs to protect the AuNPs from aggregation. Thiocyanate can be detected by the color change of the solution from blue to red. The results showed that the absorbance ratio A 675 /A 520 was linear with the concentration of SCNin the range of 0.4-1.2 μmol L-1 by UV-Vis spectroscopy. The limit of detection (LOD) of this assay was 0.37 µmol L-1. The system also had excellent selectivity and anti-interference ability. In addition, this method had been successfully used for the detection of SCNin actual water samples and had achieved good results.