Fabrication of gelatin/calcium phosphate composite nanofibrous membranes by biomimetic mineralization

Choi, Mi Ok; Kim, Young‐Jin

doi:10.1016/j.ijbiomac.2012.04.001

Cited by 39 publications

(20 citation statements)

References 37 publications

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“…The best yield of immobilization was achieved for the upper temperature of 1308 M. POHANKA incubation. It may be caused by rapidity of the monolayer formation that is a crucial factor for quality of the created gelatin membrane (Choi and Kim 2012;Yamamoto, Koike, and Dobashi 2007). Increase of gelatin concentration above 1% (w=w) has no effect on the received outputting optical density (i.e., immobilized AChE activity).…”

Section: Resultsmentioning

confidence: 99%

Spectrophotomeric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Pohanka

2013

Analytical Letters

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Aflatoxins are a group of hepatotoxic secondary metabolites produced by molds Aspergillus. During inappropriate storage or processing of food and beverages, aflatoxins can be distributed and become a serious health problem. Disparate analytical techniques were prepared for the assay in the past. Here, a method based on inhibition of enzyme acetylcholinesterase (AChE) is performed allowing fast and simple prove of aflatoxins presence. For the assay purposes, AChE was immobilized on standard polystyrene microplates using gelatin as a stabilizing substance. Both free and immobilized AChEs were used and compared one to each other. Immobilization protocol was optimized and interference of organic solvents such as methanol was tested. For the immobilized AChE, limit of detection 2.75 ppb for aflatoxin B1 was received. The immobilized AChE kept full activity at least one month. Suitability of the assay for a practical performance is considered.

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Section: Resultsmentioning

confidence: 99%

Spectrophotomeric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Pohanka

2013

Analytical Letters

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“…The uniform biomimetic fiber coatings of CaP can be obtained by mineralization of fibrous scaffold in simulated body fluid (SBF) . Similarly, CaP and CaCO 3 can be precipitated on scaffold by its incubation in salt solutions . In Ref.…”

Section: Introductionmentioning

confidence: 99%

“…28,29 Similarly, CaP and CaCO 3 can be precipitated on scaffold by its incubation in salt solutions. [30][31][32] In Ref. 33, vaterite particles were deposited on polymer foam matrix to obtain hydroxyapatite coatings to design bone tissue engineering scaffold.…”

Section: Introductionmentioning

confidence: 99%

Vaterite coatings on electrospun polymeric fibers for biomedical applications

Savelyeva

Abalymov

Lyubun

et al. 2016

J Biomedical Materials Res

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The process of porous calcium carbonate (CaCO ) covering on electrospun poly(ε-caprolactone) (PCL) fibers is described in this study. Uniform CaCO coatings, composed of vaterite microparticles and its aggregates, were formed on PCL fibers by mineral precipitation from solution under ultrasonic treatment. The porous structure of CaCO in vaterite polymorphic form is useful for loading of various substances (drugs and nanoparticles), and this property makes vaterite an appropriate material for design of drug delivery systems. Such mineralization was implemented to attain therapeutic and/or biological activity of tissue engineering scaffolds based on electrospun PCL, by means of CaCO coatings. Various structures and polymorphs of CaCO coatings were obtained by variation of growth conditions (time of fiber incubation in work solution, ultrasonic treatment of this system). Coating homogeneity, CaCO polymorphic form, morphology, and CaCO mass can be controlled by number of successive stages of fibrous material treatment. Cytotoxicity tests showed that PCL fibers mineralized with CaCO did not release substances toxic for cells. SEM images of PCL/CaCO scaffolds cultured with cells demonstrate that scaffolds supported cell adhesion and spreading. The presented results show the new technique of controlled PCL scaffold mineralization with vaterite, and an opportunity of using PCL/CaCO as scaffolds for tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 94-103, 2017.

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“…To electrospin, a 7% (wt/vol) PLLA solution was prepared in solvents dichloromethane (DCM) and dimethylformaldehyde (DMF) with 10% gelatin. Gelatin was included to promote cellular proliferation by increasing the organic collagen content of the scaffold, as well as improve the mineralization of the scaffold by acting as a binding site for calcium phosphate (Choi & Kim, ). PDLA was prepared at a concentration of 22% (wt/vol) in tetrahydrofuran (THF) and DMF (no gelatin was added).…”

Section: Methodsmentioning

confidence: 99%

Characterization of a prevascularized biomimetic tissue engineered scaffold for bone regeneration

et al. 2019

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Significant bone loss due to disease or severe injury can result in the need for a bone graft, with over 500,000 procedures occurring each year in the United States. However, the current standards for grafting, autografts and allografts, can result in increased patient morbidity or a high rate of failure respectively. An ideal alternative would be a biodegradable tissue engineered graft that fulfills the function of bone while promoting the growth of new bone tissue. We developed a prevascularized tissue engineered scaffold of electrospun biodegradable polymers PLLA and PDLA reinforced with hydroxyapatite, a mineral similar to that found in bone. A composite design was utilized to mimic the structure and function of human trabecular and cortical bone. These scaffolds were characterized mechanically and in vitro to determine osteoinductive and angioinductive properties. It was observed that further reinforcement is necessary for the scaffolds to mechanically match bone, but the scaffolds are successful at inducing the differentiation of mesenchymal stem cells into mature bone cells and vascular endothelial cells. Prevascularization was seen to have a positive effect on angiogenesis and cellular metabolic activity, critical factors for the integration of a graft.

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Fabrication of gelatin/calcium phosphate composite nanofibrous membranes by biomimetic mineralization

Cited by 39 publications

References 37 publications

Spectrophotomeric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Spectrophotomeric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Vaterite coatings on electrospun polymeric fibers for biomedical applications

Characterization of a prevascularized biomimetic tissue engineered scaffold for bone regeneration

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