Fabrication of BSA@AuNC-Based Nanostructures for Cell Fluoresce Imaging and Target Drug Delivery

Abstract

Drug delivery which can offer efficient and localized drug transportation together with imaging capabilities is highly demanded in the development of cancer theranostic approaches. Herein, we report the construction of bovine serum albumin (BSA) gold nanoclusters (BSA@AuNCs) for cell fluoresce imaging and target drug delivery. BSA@AuNCs were modified with cyclic arginine-glycine-aspartate with the product RGD-BSA@AuNCs to enhance cell internalization of the nanoclusters. Furthermore, doxorubicin hydrochloride or doxorubicin (DOX), a widely used chemotherapy drug, was also used to modify RGD-BSA@AuNCs. The final design of the DOX/RGD-BSA@AuNC system was constructed through the disulfide bond. The physical microstructure and biological characterization of the BSA@AuNCs were realized through high-resolution transmission electron microscopy and confocal laser fluorescence microscopy. As the disulfide bonds were cleaved by glutathione in cancer cells, DOX-SH molecules were released from the nanosystem to inhibit the growth of cancer cells. The as-prepared DOX/RGD-BSA@AuNC system can be used not only to deliver drug but also to achieve the antitumor effect by in vivo imaging, demonstrating its promising applications in cancer treatment.

High antiviral activity of mercaptoethane sulfonate functionalized Te/BSA nanostars against arterivirus and coronavirus

“…BSA is a common material used to synthesize nanoparticles due to its novel biological activity. 72,73 Especially, BSA-based nanomaterials are widely applied in biomedicine for their good properties of drug load. For example, doughnut-shaped BSA nanoparticles loaded with doxorubicin are utilized to overcome multidrug resistance during cancer treatment.…”

Supramolecules‐Guided Synthesis of Brightly Near‐Infrared Au22 Nanoclusters with Aggregation‐Induced Emission for Bioimaging

“…[28] For example, multiplex identification of bacteria by their inherited binding affinities with bacteria, [29] rapid detection of insulin by their enhanced functionality, [30,31] and tumor imaging by their unique emission wavelength. [31] But these applications often require high PL intensity, the relatively low PL intensity (usually lower than 6%) still existing in BSA-AuNCs extremely limits their developments. This is mainly because how reaction factors participate in the formation process and affect the optical properties of BSA-AuNCs is still unclear.…”

“…This green synthesis method give BSA‐AuNCs unique physicochemical properties, improving their prospects for biological applications . For example, multiplex identification of bacteria by their inherited binding affinities with bacteria, rapid detection of insulin by their enhanced functionality, and tumor imaging by their unique emission wavelength . But these applications often require high PL intensity, the relatively low PL intensity (usually lower than 6%) still existing in BSA‐AuNCs extremely limits their developments.…”

Facile green synthesis of bismuth sulfide radiosensitizer via biomineralization of albumin natural molecule for chemoradiation therapy aim

“…Furthermore, bovine serum albumin (BSA) has received more attention than other biological carrier, especially in cancer treatment because of their unique properties [8]. Rarely toxicity or immunogenicity; biodegradable; stable in circulation; qualifying a long half-life; does not require surfactants or polymeric materials for preparation; easy access to all tumour sites via the blood circulation; high chemical stability; and convenient to administer are likely properties to persuade researcher to use Albumin eagerly.…”