Fabrication of Antibacterial Collagen-Based Composite Wound Dressing

Abstract

Collagen is a favorable candidate in the field of biomaterials as a wound dressing. However, it cannot be ignored that the application of collagen is limited to its poor physicochemical and perishable properties. It is significant to endow collagen with antibacterial activity and simultaneously improve its physicochemical properties. Here, we present a simple method to fabricate antibacterial collagen-based wound dressing with appropriate physicochemical properties. First, dialdehyde xanthan gum (DXG) was used as an environmentally friendly reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs). Then, collagen/DXG-AgNP composite dressings were fabricated by directly immersing a collagen sponge in the obtained DXG-AgNP aqueous solutions. Our results showed that the spherical AgNPs with diameters of 12–35 nm were successfully synthesized. The presence of DXG effectively prevented aggregation and precipitation of AgNPs in aqueous solution. By the simple one-step solution-immersion approach, AgNPs were homogeneously introduced into the collagen matrix and collagen was simultaneously cross-linked by the existent DXG. Robust antibacterial activity was endowed to collagen as expected while the physicochemical properties of collagen were effectively improved. It is interesting that collagen/DXG-AgNP composite dressings possessed the properties of shape memory, good blood compatibility, and cytocompatibility. In addition, collagen/DXG-AgNP composite dressings could accelerate the deposition of collagen and thereby effectively promote full-thickness burn healing without scar formation.

Influence of the weight ratio of polydimethylsiloxane modified gelatin to silicone rubber on the potential performance of asymmetric bilayer membranes as wound dressings

“…With regard to the mechanical properties in the dry state, in comparison with our previous work (PGE/SR, σ b = 4.9 MPa, ϵ b = 15.1%), and the other previously reported wound dressings such as collagen/dialdehyde xanthan gum silver nanoparticle composite (Col‐Ag2, σ b = 0.512 MPa, ϵ b ≈ 13%), poly(vinyl alcohol)/chitosan asymmetrical membrane (PVA/CS_10min, σ b = 0.7 MPa, ϵ b = 21%), bilayer chitosan‐gelatin ( σ b = 0.5–1.1 MPa) and electrospun GE mat ( σ b = 1.6 MPa, ϵ b = 17%), the prepared PGE/SR1 bilayer membrane in the present work possessed competitive flexibility, rigidity and toughness, and was capable of protecting dressings from mechanical damage.…”

“…Strikingly, the rest of the prepared membranes showed a reduction in σ b and an increment in ϵ b compared to the corresponding membranes in the dry state. This phenomenon can probably be attributed to the presence of water molecules in the wet GE‐containing membranes, acting as a plasticizer . In particular, the PGE/SR1 (wet state) exhibited a balanced σ b (0.6 MPa) and ϵ b (48.0%) compared with the other membranes.…”

Physicochemical Characterization of Bilayer Hybrid Nanocellulose-Collagen as a Potential Wound Dressing

“…Hence, intermolecular crosslinking was sought to modify the collagen scaffold’s mechanical strength and to preserve its stability, thus correcting such limitation [ 15 ]. Studies showed that collagen scaffold crosslinked with genipin have a better mechanical profile without having its porosity properties affected and have minimal toxicity [ 48 ]. This synergistic improvement suggests good chemical compatibility between the two phases, as well as good dispersion [ 49 ].…”

Porous organosilicone modified gelatin hybrids with controllable and homogeneous in vitro degradation behaviors for potential application as skin regeneration scaffold

“…It therefore makes sense that the present work provides a viable avenue to tune the gelatin hybrid from soft (hard) to hard (soft). In comparison with the previously reported biopolymer‐based wound dressing scaffolds (dry state) such as collagen bio‐artificial dermis ( σ b = 1.5 MPa), collagen/dialdehyde xanthan gum‐silver nanoparticles composite (Col‐Ag2, σ b = 0.512 MPa, ϵ b ≈ 13%), electrospun gelatin mat ( δ = 1.6 MPa, ϵ = 17%) and porous gelatin scaffolds (Gf‐2.0, δ = 7.8 kPa), the P1G1‐GE membrane ( δ = 6.1 MPa, ϵ = 9.4%) described here possessed appropriate rigidity and flexibility, which allowed the dressings to fit well in the wound sites.…”