Fabrication and characterization of genipin cross-linked chitosan/gelatin hydrogel for pH-sensitive, oral delivery of metformin with an application of response surface methodology

Ubaid, Muhammad; Murtaza, Ghulam

doi:10.1016/j.ijbiomac.2018.04.023

Cited by 56 publications

(41 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, regarding film elongation (Figure 1f), namely yield at break, the value ranges from 57.3 to 77.4%. The introduction of cross-linkers caused an increase in both film strength and elongation, which could be attributed to the improvement of the interaction among matrix polymers [42]. Additionally, the cross-linker mixture performed best in terms of improving the film strength.…”

Section: Resultsmentioning

confidence: 99%

The Impact of Cross-linking Mode on the Physical and Antimicrobial Properties of a Chitosan/Bacterial Cellulose Composite

2019

View full text Add to dashboard Cite

The bacteriostatic performance of a chitosan film is closely related to its ionic and physical properties, which are significantly influenced by the mode of cross-linking. In the current work, chitosan with or without bacterial cellulose was cross-linked with borate, tripolyphosphate, or the mixture of borate and tripolyphosphate, and the composite films were obtained by a casting of dispersion. Mechanical measurements indicated that different modes of cross-linking led to varying degrees of film strength and elongation increases, while the films treated with the borate and tripolyphosphate mixture showed the best performance. Meanwhile, changes in the fractured sectional images showed a densified texture induced by cross-linkers, especially for the borate and tripolyphosphate mixture. Measurements of Fourier transform infrared showed the enhanced interaction between the matrix polymers treated by borate, confirmed by a slight increase in the glass transitional temperature and a higher surface hydrophobicity. However, the reduced antimicrobial efficiency of composite films against E. coli, B. cinerea, and S. cerevisiae was obtained in cross-linked films compared with chitosan/bacterial cellulose films, indicating that the impact on the antimicrobial function of chitosan is a noteworthy issue for cross-linking.

show abstract

Section: Resultsmentioning

confidence: 99%

The Impact of Cross-linking Mode on the Physical and Antimicrobial Properties of a Chitosan/Bacterial Cellulose Composite

2019

View full text Add to dashboard Cite

show abstract

“…[24] Additionally, just like full-IPNs, semi-IPNs have the advantages of tunable pore size and controlled drug release. [15,23,24] A range of natural and synthetic polymers have been explored as a polymeric component in semi-IPN chitosan-genipin hydrogels, including gelatin, [25] alginate, [26] and polyvinylpyrrolidone. [27] Of the polymers that can be incorporated into chitosan-crosslinked networks to yield semi-IPNs, poly(ethylene) glycol (PEG) ( Figure 1) attracted our attention.…”

Section: Introductionmentioning

confidence: 99%

Poly(ethylene glycol)‐interpenetrated genipin‐crosslinked chitosan hydrogels: Structure, pH responsiveness, gelation kinetics, and rheology

Huang

Lemos

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

In the development of pH‐responsive chitosan‐based hydrogels, achieving reproducible porosity and swelling behavior is essential for the design of hydrogel networks. Herein, we enhance the level of control in hydrogel microarchitecture by incorporating poly(ethylene glycol) (PEG) into the chitosan–genipin matrix. Hydrogels, varied in composition, were synthesized under mild conditions (37°C, 1 atm, 24 hr), yielding microporous structures with a pore diameter ranging from 11 to 57 μm and an average cross‐sectional porosity of approximately 40–64%. Compared to chitosan–genipin hydrogels without PEG, presence of PEG in concentrations up to 1.9 mM generated the same effect as would increase in genipin content, yielding structures with a smaller pore diameter, a lower swelling degree in pH 2 buffer and a higher elastic modulus. Considering cost effectiveness and scale‐up, reducing genipin content by the addition of PEG is favorable. Importantly, hydrogel samples containing higher concentrations of PEG (2.9 mM and above) showed a sudden increase in the swelling degree accompanied with a decrease in the elastic modulus. Findings showcase the potential variation in the composition of these hydrogels has in yielding scaffolds with significantly different physico‐chemical behaviors.

show abstract

“…might be involved in the self‐healing process (Tu et al, ). Genipin was likely to promote the formation of secondary amide, which resulted from reaction between ester group of genipin and the amino group of chitosan (Ubaid & Murtaza, ). According to our findings, acylhydrazone bonds were significantly increased, whereas the alcohol group lessened, suggesting that chemical reaction between ester and amino groups occurred in this self‐healing process.…”

Section: Discussionmentioning

confidence: 99%

“…might be involved in the self-healing process (Tu et al, 2019). Genipin was likely to promote the formation of secondary amide, which resulted from reaction between ester group of genipin and the amino group of chitosan (Ubaid & Murtaza, 2018).…”

Section: Discussionmentioning

confidence: 99%

Genipin‐crosslinked decellularized annulus fibrosus hydrogels induces tissue‐specific differentiation of bone mesenchymal stem cells and intervertebral disc regeneration

Peng

Huang

et al. 2020

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Biomaterial‐based therapy that can restore annulus fibrosus (AF) function in early stage and promote endogenous repair of AF tissues is a promising approach for AF tissue repair. In this study, we established a genipin‐crosslinked decellularized AF hydrogels (g‐DAF‐G) that are injectable and could manifest better in situ formability than noncrosslinked decellularized AF hydrogel, while preserving the capacity of directing differentiation of human bone mesenchymal stem cells (hBMSCs) towards AF cells. Hematoxylin and eosin staining, 4',6‐diamidino‐2‐phenylindole staining, and so forth showed that the majority of cellular components were removed, whereas extracellular matrix and microstructure were largely preserved. The storage modulus increased from 465.5 ± 9.4 Pa to 3.29 ± 0.24 MPa after 0.02% genipin crosslinking of decellularized AF hydrogels (DAF‐G) to form g‐DAF‐G. AF‐specific genes (COL1A1, COL5A1, TNMD, IBSP, FBLN1) were significantly higher in DAF‐G and g‐DAF‐G groups than that in control group after 21 days of culturing. g‐DAF‐G significantly restored nucleus pulposus water content and preserved intervertebral structure in vivo. Summarily, we produced a novel AF regeneration biomaterial, g‐DAF‐G, which exhibited well biocompatibility, great bioactivity, and much higher mechanical strength than DAF‐G. This study will provide an easy and fast therapeutic alternative to repair AF injuries or tears.

show abstract

Fabrication and characterization of genipin cross-linked chitosan/gelatin hydrogel for pH-sensitive, oral delivery of metformin with an application of response surface methodology

Cited by 56 publications

References 41 publications

The Impact of Cross-linking Mode on the Physical and Antimicrobial Properties of a Chitosan/Bacterial Cellulose Composite

The Impact of Cross-linking Mode on the Physical and Antimicrobial Properties of a Chitosan/Bacterial Cellulose Composite

Poly(ethylene glycol)‐interpenetrated genipin‐crosslinked chitosan hydrogels: Structure, pH responsiveness, gelation kinetics, and rheology

Genipin‐crosslinked decellularized annulus fibrosus hydrogels induces tissue‐specific differentiation of bone mesenchymal stem cells and intervertebral disc regeneration

Contact Info

Product

Resources

About