Fabrication and bioseparation studies of adsorptive membranes/felts made from electrospun cellulose acetate nanofibers

Zhang, Lifeng; Menkhaus, Todd J.; Fong, Hao

doi:10.1016/j.memsci.2008.03.030

Cited by 139 publications

(102 citation statements)

References 33 publications

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“…26 Briefly, bovine serum albumin (BSA) was used as a model protein for adsorption experiments with diethylaminoethyl (DEAE) surface functionalized nanofibers. It was found that the static binding capacity was 40 mg/g, approximately 20% higher than a commercial membrane adsorption product.…”

Section: Membrane Adsorber Parametersmentioning

confidence: 99%

“…[22][23][24][25] However, the binding capacity of these membrane adsorbers has been reported to be significantly lower when compared with the binding capacities of similar resins. 26 To overcome the capacity limitation of membrane adsorbers, electrospun cellulose acetate nanofibrous membranes with nanofiber diameter of $500 nm have been developed. 26,27 The nanofibrous membranes have been subjected to either surface functionalization to allow for protein adsorption directly on the surface of the fiber 26 or atomic transfer radical polymerization (ATRP) 27 to create grafted tethers for adsorption of protein.…”

Section: Introductionmentioning

confidence: 99%

“…26 To overcome the capacity limitation of membrane adsorbers, electrospun cellulose acetate nanofibrous membranes with nanofiber diameter of $500 nm have been developed. 26,27 The nanofibrous membranes have been subjected to either surface functionalization to allow for protein adsorption directly on the surface of the fiber 26 or atomic transfer radical polymerization (ATRP) 27 to create grafted tethers for adsorption of protein. In this fashion, functionalized nanofibers capable of various interactions (i.e., affinity, IEX, and HIC) can, and have been, produced.…”

Section: Introductionmentioning

confidence: 99%

“…[28][29][30] Higher binding capacities and permeabilities, accompanied by reduced residence times for interactions, are reported for electrospun regenerated cellulose nanofiber membranes when compared with the packed bed resins. 26,27 These factors can lead to dramatic improvements in purification time, cost, and significantly reduce waste generation not only for the membrane adsorption operation but also for other downstream operations such as ultrafiltration and nanofiltration. Furthermore, by replacing the packed bed adsorption/ chromatography steps with alternative membrane materials, the combined downstream purification process can be transformed into one using only membranes.…”

Section: Introductionmentioning

confidence: 99%

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Process and economic evaluation for monoclonal antibody purification using a membrane‐only process

Varadaraju

Schneiderman

Zhang

et al. 2011

Biotechnology Progress

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In recent years, the market for therapeutic monoclonal antibodies (mAb) has grown exponentially, and with this there has been a desire to reduce the costs associated with production and purification of these high-value biological products. A typical mAb purification process involves three adsorption/chromatography steps [protein A, ion exchange (IEX), and hydrophobic interaction (HIC)], along with ultrafiltration, nanofiltration, and microfiltration. With the development of membrane adsorption/chromatography as a viable alternative to traditional pack bed systems, the opportunity exists to complete the entire downstream purification process using only membrane operations. In this study, the process simulation tool SuperPro Designer was used to evaluate the application of recently developed ultra-high capacity electrospun nanofibrous adsorption membranes as a replacement for conventional chromatographic media in the downstream mAb production process. The simulation showed that nanofibrous adsorption membranes in place of the three packed bed chromatography steps reduced the required volume of protein A, IEX, and HIC adsorptive medium by 25, 80, and 80%, respectively. In addition, the membrane-only process reduced the downstream processing time by 50%, decreased the number of labor hours associated with the purification steps by 40%, generated 40% less aqueous waste, and reduced the overall downstream process operating expenses per unit product by 23%. There were also significant savings in facility construction costs and the price of fixed equipment required for separations. With these savings not only is the membrane-only process economically competitive with the traditional packed bed operations, but it offers the possibility of moving toward more disposable process.

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Section: Membrane Adsorber Parametersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Process and economic evaluation for monoclonal antibody purification using a membrane‐only process

Varadaraju

Schneiderman

Zhang

et al. 2011

Biotechnology Progress

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“…Porous membrane adsorbers have been successfully applied in lab-scale [1] and large-scale antibody production for trace contaminant removal and virus clearance [2,3]. To date, much research for improvement of membrane adsorber separation performance has been focused on materials such as base membranes that are more suitable and efficient [4,5], improved surface chemistries [6][7][8][9], on transport mechanisms [10][11][12][13][14][15], as well as on correlations between materials and transport mechanisms [16]. Some particularly effective improvements have been achieved by novel membrane module designs to optimize flow distribution of membrane devices on a lab- [17][18][19][20][21] and production-scale [22,23].…”

Section: Introductionmentioning

confidence: 99%

Model Based Quantification of Internal Flow Distributions from Breakthrough Curves of Flat Sheet Membrane Chromatography Modules

2010

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A novel model for quantifying radial flow distributions in flat sheet membrane chromatography modules under non-binding conditions is presented and applied for the practical analysis of two modules. The proposed model partitions the total void volume of the chromatography module into zones that are considered homogeneous with respect to flow velocity. The corresponding solute concentrations are time variant, but also spatially homogeneous within each zone. The model is mathematically represented and analytically solved as a network of continuously stirred tank reactors (CSTR). An additional plug flow reactor (PFR) is connected in series with the CSTR network in order to account for a time-lag that is not associated with the system dispersion. The capability of the model to describe experimental breakthrough data is compared to the frequently applied standard model for extra-membrane system dispersion, which consists of a single CSTR in series with a PFR. Non-binding conditions are deliberately chosen for studying the impact of module geometries on breakthrough curves separately from chromatographic membrane performance. The commercial CIM module and a custom designed cell (Scell) are studied with acetone and lysozyme as test tracers at varied flow rates and for various membrane pore sizes under non-binding conditions. In all studied cases, the proposed model fits the measured breakthrough curves better than the standard model. Moreover, the minimal number of radial flow zones that are required to accurately describe observed breakthrough curves and the estimated flow fractions through these zones provide valuable information for the analysis and optimization of internal module designs.

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Ceramic‐Based Adsorbents in Bioproduct Recovery and Purification

Fernández‐Lahore

Yelemane

Islam

et al. 2022

Surface‐Functionalized Ceramics

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Fabrication and bioseparation studies of adsorptive membranes/felts made from electrospun cellulose acetate nanofibers

Cited by 139 publications

References 33 publications

Process and economic evaluation for monoclonal antibody purification using a membrane‐only process

Process and economic evaluation for monoclonal antibody purification using a membrane‐only process

Model Based Quantification of Internal Flow Distributions from Breakthrough Curves of Flat Sheet Membrane Chromatography Modules

Ceramic‐Based Adsorbents in Bioproduct Recovery and Purification

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