F25P preproinsulin abrogates the secretion of pro-growth factors from EGFRvIII cells and suppresses tumor growth in an EGFRvIII/wt heterogenic model

Signal Peptide Peptidase, Encoded by HM13, Contributes to Tumor Progression by Affecting EGFRvIII Secretion Profiles in Glioblastoma

“…Approximately 3–5% of all membrane proteins are tail‐anchored proteins , and signal peptide peptidase (SPP), which catalyzes the intramembrane cleavage of signal sequence stubs left in the ER membrane after signal peptidase (SP)–mediated processing of the precursor protein, plays an important role in the maturation of these proteins . Previously, we had identified that specifically inhibiting the activity of signal peptidase could suppress tumor growth in an EGFRvIII/wt heterogenic model by abrogating the secretion of progrowth factors from EGFRvIII cells . Here, we analyzed the relative gene expression and survival period from TCGA, CGGA, REMBRANDT, and GSE16011 databases, knocked down HM13 gene, and established an intracranial model in mice to investigate the effect of HM13 on EGFRvIII secretion profiles and tumor progression (Figure ).…”

The interactome and spatial redistribution feature of Ca2+ receptor protein calmodulin reveals a novel role in invadopodia-mediated invasion

“…Paraffin-embedded tissues used for performing H&E staining and IHC analysis were prepared as described previously 44 . For performing IHC analysis, the paraffin-embedded tissues were cut into 6 μm-thick slices, fixed on a glass slide, deparaffinized in xylene, rehydrated in graded ethanol, treated with 0.3% hydrogen peroxide (ZSGB-Bio, China) for quenching endogenous peroxidase activity, and heated in an all-purpose powerful antigen retrieval solution (Beyotime, China) at 37 °C for antigen retrieval.…”