F200Y polymorphism in the β-tubulin gene in field isolates of Haemonchus contortus and risk factors of sheep flock management practices related to anthelmintic resistance

Niciura, S. C. M.; Verı́ssimo, Cecı́lia José; Gromboni, Juliana Gracielle Gonzaga; Rocha, M. I. P.; Mello, Sarita V.; Barbosa, Cristina Maria Pacheco; Chiebáo, Daniela Pontes; Cardoso, Daniel; Silva, Giane Serafim; Otsuk, Ivani Pozar; Pereira, José Roberto; Ambrósio, Luís Alberto; Nardon, Romeu Fernandes; Ueno, Tatiana Evelyn Hayama; Molento, Marcelo Beltrão

doi:10.1016/j.vetpar.2012.07.016

Cited by 43 publications

(22 citation statements)

References 19 publications

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“…Figure 1 shows a representative result from AS-PCR, including the appropriate controls used for validation of the method. High levels of resistance to BZ have recently been reported in several herds in Brazil (CRUZ et al, 2010;NICIURA et al, 2012;VERÍSSIMO et al, 2012). Furthermore, the higher frequency of ATH treatment in the SP1 herd (4 or more treatments/year) might also explain the higher frequency of the R alleles obtained (Table 2).…”

Section: Resultsmentioning

confidence: 90%

“…Genotyping of the polymorphism TTC/TAC at position 200 of isotype 1 of the β-tubulin gene was performed on at least 10 specimens from each host, by means of a Haemonchus sp. specific reaction of AS-PCR, as described by Niciura et al (2012). Plasmids containing cloned β-tubulin1 alleles bearing either the TTC200 (S allele) or the TAC200 (R allele) SNPs previously obtained by Brasil et al (2012) were included as controls.…”

Section: Methodsmentioning

confidence: 99%

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Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Nunes

Santos

Bastianetto

et al. 2013

Rev. Bras. Parasitol. Vet.

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Anthelmintic resistance is an increasing problem that threatens livestock production worldwide. Understanding of the genetic basis of benzimidazole resistance recently allowed the development of promising molecular diagnostic tools. In this study, isolates of Haemonchus contortus obtained from goats, sheep and buffaloes raised in Brazil were screened for presence of the polymorphism Phe200Tyr in the β-tubulin 1 gene, which confers resistance to benzimidazole. The allelic frequency of the mutation conferring resistance ranged from 7% to 43%, and indicated that resistance to benzimidazole could be found in nematodes isolated from all the ruminant species surveyed. Although significant variation in the frequency of the F200Y mutation was observed between different herds or host species, no significant variation could be found in populations isolated from animals within the same herd. These findings suggest that screening of samples from a few animals has the potential to provide information about the benzimidazole resistance status of the entire herd, which would enable a considerable reduction in the costs of diagnosis for the producer. Molecular diagnosis has practical advantages, since it can guide the choice of anthelmintic drug that will be used, before its application in the herd, thus reducing the economic losses driven by anthelmintic resistance.Keywords: Anthelmintic resistance, benzimidazole drugs, molecular diagnosis, domestic ruminants, genetic polymorphisms. ResumoA resistência aos anti-helmínticos é um problema crescente que ameaça a produção pecuária em todo o mundo. A compreensão da base genética da resistência ao benzimidazol permitiu, recentemente, o desenvolvimento de métodos diagnósticos moleculares promissores. Neste estudo, isolados de Haemonchus contortus obtidos a partir de rebanhos de caprinos, ovinos e bubalinos criados no Brasil foram avaliados quanto à presença do polimorfismo F200Y no gene da β-tubulina1, o qual confere resistência ao benzimidazol. A frequência alélica da mutação variou de 7% a 43%, indicando que a resistência ao benzimidazol pode ser encontrada em nematoides isolados a partir de todas as espécies de ruminantes pesquisadas. Embora tenha sido observada variação significativa das frequências de mutação F200Y entre rebanhos/espécies hospedeiros distintos, não foi encontrada variação significativa entre populações isoladas de animais dentro de um mesmo rebanho. Estes achados sugerem que a avaliação de amostras de alguns poucos animais tem o potencial de fornecer informações sobre o nível de resistência ao benzimidazol de todo o rebanho, possibilitando uma redução considerável dos custos de diagnóstico para o produtor. O diagnóstico molecular apresenta vantagens práticas, uma vez que pode guiar a escolha da base anti-helmíntica a ser utilizada antes da sua aplicação no rebanho, reduzindo, portanto, as perdas ocasionadas pela resistência aos fármacos anti-helmínticos.Palavras-chave: Resistência a anti-helmínticos, benzimidazóis, diagnóstico molecular, ruminantes domés...

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Section: Resultsmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Nunes

Santos

Bastianetto

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…The putative protective factors included: treatment of animals during quarantine (Suter et al, 2004), the use of combination anthelmintics Niciura et al, 2012) and selective treatment of animals based on the lambs' weight (i.e. heaviest animals left untreated) .…”

Section: Discussionmentioning

confidence: 99%

“…heaviest animals left untreated) . Putative risk factors included: under-dosing of animals (Calvete et al, 2012;Niciura et al, 2012), annual rotation of anthelmintic drug classes (Suter et al, 2004;Leignel et al, 2010), rotational grazing , treatment of peri-parturient ewes , and not calibrating the drench gun to ensure that it is administering the correct anthelmintic dose (Hughes et al, 2007). Currently, use of combination anthelmintics is a particularly controversial topic, as some studies suggest that it slows the development of AHR Bartram et al, 2012;Learmount et al, 2012), while others argue the opposite as cross-resistance between drug classes has been observed (Use of Drug Combinations, 2007).…”

Section: Discussionmentioning

confidence: 99%

A systematic review and meta-analysis of factors associated with anthelmintic resistance in sheep

Falzon

O’Neill

Menzies

et al. 2014

Preventive Veterinary Medicine

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“…Almost 100% of the farmers said that they used visual weight estimates and not measured weights (Table 3). Niciura et al (2012) found that visual weight estimates for flocks of sheep were the main risk factor for the presence of resistant Haemonchus contortus. During a recent survey on ivermectin resistance on Mexican farms, Alegria-López et al (2015) found that use of ivermectin promoted development of ivermectin resistance among both nematodes and cattle ticks.…”

Section: Discussionmentioning

confidence: 99%

Management and control of parasites on dairy farms in northwestern region of São Paulo state

Verı́ssimo

Vasques

Duarte

et al. 2016

Rev. Bras. Parasitol. Vet.

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Dairy cattle farming is of great economic and social importance in all Brazilian's regions. Parasites can reduce milk productivity, especially the tick Rhipicephalus (Boophilus) microplus. This study consisted of a questionnaire answered by 40 milk producers in the northwestern region of the State of São Paulo. The aim was to ascertain how these producers controlled ticks and other parasites. Very many of them knew nothing about the biological cycle of the cattle tick or about strategic control or acaricide efficacy tests. The majority (87.5%) controlled ticks at a high frequency, without technical criteria and care to apply the acaricide. Spraying was the most used mode of acaricide application (95%) and endectocides were used by 45%. Cattle tick fever was the harm most associated with ticks (87.5%) followed closely by screwworm (77.5%). However, 65% were satisfied with their tick control. About the control of others parasites, all dewormed at least twice a year their animals; 65% were controlling horn fly; 40% had problems with screwworm. The interviewers had in general good level of education and the farms generally exhibited a high degree of technology for milk production on pasture because half of them received technical assistance frequently.Keywords: Cattle, milk, production, questionnaire, sustainable, tick. ResumoA bovinocultura leiteira é de extrema importância econômica e social em todas as regiões brasileiras. Os parasitas podem reduzir a produção leiteira, especialmente o carrapato Rhipicephalus (Boophilus) microplus. Este trabalho consistiu em um questionário respondido por 40 produtores de leite da região noroeste do Estado de São Paulo, com o objetivo de saber como os produtores controlam carrapatos e outros parasitas. Grande parte deles não conhecia o ciclo biológico do carrapato do boi nem o controle estragégico e o teste de eficácia de carrapaticidas. A maioria (87,5%) controlava o carrapato com alta frequência, sem critérios técnicos e cuidados na aplicação do carrapaticida. A aspersão foi o modo de aplicação mais usado (95%) e endectocidas eram utilizados por 45%. Tristeza parasitária bovina foi o prejuízo mais associado ao carrapato (87,5%), seguido de perto pela bicheira (77,5%). No entanto, 65% estavam satisfeitos com o controle do carrapato. A maioria (82,5%) criava gado mestiço. Em relação ao controle de outros parasitas, todos vermifugavam seus animais pelo menos duas vezes por ano; 65% deles controlava a mosca-do-chifre; 40% têm problemas com bicheira. Os entrevistados, de forma geral, tinham bom nível educacional, e as fazendas geralmente exibiam alto grau de tecnologia em produção de leite a pasto, porque metade delas recebia assistência técnica constante de um programa estatal que incentiva a produção leiteira sustentável.Palavras-chave: Bovino, leite, produção, questionário, sustentável, carrapato.

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F200Y polymorphism in the β-tubulin gene in field isolates of Haemonchus contortus and risk factors of sheep flock management practices related to anthelmintic resistance

Cited by 43 publications

References 19 publications

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

A systematic review and meta-analysis of factors associated with anthelmintic resistance in sheep

Management and control of parasites on dairy farms in northwestern region of São Paulo state

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