EZH2 is a potential prognostic predictor of glioma

Chen, Yinan; Hou, Shiqiang; Jiang, Rui; Sun, Junlong; Chen, Cheng; Zhou, Qian

doi:10.1111/jcmm.16149

Cited by 37 publications

(29 citation statements)

References 57 publications

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“…We further explored the potential relationships between NOP2 and immunity mainly including four aspects (tumor microenvironment, tumor immune infiltration, immune cell pathway, and immune checkpoint molecules). As reported by previous articles, tumor microenvironment as well as tumor immune infiltration was associated with ccRCC prognosis and response to immunotherapy [ 49 , 50 ] and various genes had been found to be significantly associated with immune cells pathways and checkpoint molecules [ 51 , 52 ]. As for tumor immune infiltration, NOP2 was significantly associated with CD4 + T cells, B cells, neutrophil cells, CD8 + T cells and dendritic cells infiltration.…”

Section: Discussionmentioning

confidence: 86%

Nucleolar protein NOP2 could serve as a potential prognostic predictor for clear cell renal cell carcinoma

Wang

Liu

et al. 2021

Bioengineered

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As an indispensable part for cancer precision medicine, biomarkers and signatures for predicting cancer prognosis and therapeutic benefits were urgently required. The purpose of this study was to investigate the prognostic roles of NOP2 in renal clear cell carcinoma (ccRCC) for overall survival (OS) and its relationships with immunity. NOP2-related gene expression matrix associated with clinical information was obtained from the Cancer Genome Atlas (TCGA) ccRCC dataset and NOP2-related pathways were identified by gene set enrichment analysis (GSEA). Associations among the NOP2 expression and MSI, TMB, TNB, and immunity were also explored. Both the NOP2 mRNA and protein/phosphoprotein had a higher expression in ccRCC tumor tissues than in normal kidney tissues (both P < 0.001) and elevated NOP2 expression was associated with poor OS (P < 0.001). Logistic regression analysis revealed the NOP2 expression was significantly linked to stage, age, grade, N stage, T stage, and M stage (all P < 0.05). Univariate/multivariate Cox hazard regression analysis results indicated that NOP2 was an independent prognostic factor for OS in ccRCC and GSEA revealed five NOP2-related signaling pathways. Nomogram based on NOP2 and eight clinical characteristic parameters (grade, age, stage, gender, T stage, race, M stage, N stage) was constructed and carefully evaluated. Furthermore, NOP2 gene expression was also found to be significantly related to MSI, TMB, and immunity. Our findings revealed that NOP2 might be a potential prognostic factor for OS in ccRCC and it was significantly associated with immunity, MSI, and TMB.

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Section: Discussionmentioning

confidence: 86%

Nucleolar protein NOP2 could serve as a potential prognostic predictor for clear cell renal cell carcinoma

Wang

Liu

et al. 2021

Bioengineered

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“…ese data reflected the heterogeneity of tumor immune microenvironment between high and low ICI score LGG tissues. Immunotherapies with blockage of immune checkpoints have displayed clinical efficacy in LGG [37]. Here, high ICI scores were characterized by decreased immune activity genes including GZMA, TBX2, TNF, PRF1, IFNG, CXCL9, and CXCL10 as well as reduced immune checkpoint genes including LAG3, CD274, IDO1, PDCD1, HAVCR2, and CTLA4.…”

Section: Discussionmentioning

confidence: 96%

Uncovering the Immune Cell Infiltration Landscape in Low-Grade Glioma for Aiding Immunotherapy

Yang

Tian

et al. 2022

Journal of Oncology

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Objective. Low-grade glioma (LGG) mainly threatens the elderly population, with undesirable prognoses. This study uncovered the immune cell infiltration (ICI) landscape in LGG. Methods. RNA-seq profiles of LGG were retrieved from TCGA and CGGA databases. CIBERSORTx and ESTIMATE algorithms were employed to characterize the ICI landscape in LGG tissues. Through unsupervised clustering analysis, ICI subtypes were clustered. ICI scores were computed via principal component analysis (PCA). The differences in survival, tumor-infiltrating immune cells, stromal scores, immune scores, immune checkpoint genes, immune activity genes, and tumor mutation burden (TMB) were assessed between high and low ICI score groups. Results. Three ICI subtypes were constructed in LGG, with distinct survival outcomes, PD-L1 expression, and infiltration levels of immune cells. Furthermore, ICI scores were developed. Both in TCGA and CGGA datasets, low ICI scores were indicative of undesirable outcomes. High ICI scores were significantly correlated to increased infiltration levels of memory B cells, CD8 T cells, CD4 naïve T cells, T follicular helper cells, macrophages M0, and eosinophils, while low ICI scores were characterized by increased infiltration levels of naïve B cells, plasma cells, CD4 memory resting T cells, Tregs, resting NK cells, macrophages M2, and activated dendritic cells. High ICI scores exhibited correlations with lower immune activity genes and immune checkpoint genes. Furthermore, TMB was distinctly reduced in the high ICI score group. Conclusion. The ICI scores may serve as a promising prognostic index and predictive indicator for immunotherapies, extending our understanding of immune microenvironment in LGG.

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“…Studies have also reported that EZH2 is involved in a novel miR-490-3p/TGIF2/TGFBR1 axis inducing migration and EMT in glioblastomas [15]. EZH2 is also connected with glioma proliferation and metastasis and has been regarded as a potential predictor and therapeutic target in glioma [26,27]. In general, EZH2 could be a marker associated with EMT in glioma.…”

Section: Discussionmentioning

confidence: 99%

H3.3K27M Mutation Promotes The Migration and Invasion of Glioma Cells By Activating β-Catenin/USP1 Signaling

Sun

Zhu

Xia

et al. 2022

Preprint

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H3.3K27M is a newly identified molecular pathology marker in glioma and is especially correlated with the malignancy of diffuse intrinsic pontine glioma (DIPG). In recent years, accumulating research has revealed that other types of glioma also contain the H3.3K27M mutation. However, the role of H3.3K27M in high-grade adult glioma, which is the most malignant glioma, has not been investigated. In this study, we focused on exploring the expression and function of H3.3K27M in high-grade adult glioma patients. We found that H3.3K27M is partly highly expressed in high-grade glioma tissues. Then, we introduced H3.3K27M into H3.3 wild-type glioma cells, U87 cells and LN229 cells. We found that H3.3K27M did not regulate the growth of glioma in vitro and in vivo; however, the survival of mice with transplanted tumors was significantly reduced. Further investigation revealed that H3.3K27M expression mainly promoted the migration and invasion of glioma cells. Moreover, we certified that H3.3K27M overexpression enhanced the protein levels of ꞵ-catenin and p-ꞵ-catenin, the protein and mRNA levels of ubiquitin-specific protease 1 (USP1), and the protein level of enhancer of zeste homolog 2 (EZH2). Importantly, the ꞵ-catenin inhibitor XAV-939 significantly attenuated the upregulation of the aforementioned proteins. Overall, the H3.3K27M mutation is present in a certain proportion of high-grade glioma patients and facilitates a poor prognosis by promoting the metastasis of glioma by regulating the ꞵ-catenin/USP1/EZH2 pathway.

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EZH2 is a potential prognostic predictor of glioma

Abstract: Zhong-run Qian 1 This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 37 publications

References 57 publications

Nucleolar protein NOP2 could serve as a potential prognostic predictor for clear cell renal cell carcinoma

Nucleolar protein NOP2 could serve as a potential prognostic predictor for clear cell renal cell carcinoma

Uncovering the Immune Cell Infiltration Landscape in Low-Grade Glioma for Aiding Immunotherapy

H3.3K27M Mutation Promotes The Migration and Invasion of Glioma Cells By Activating β-Catenin/USP1 Signaling

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