Extremely simple single-prism ultrashort- pulse compressor

Aktürk, Selçuk; Gu, Xun; Kimmel, Mark W.; Trebino, Rick

doi:10.1364/oe.14.010101

Cited by 93 publications

(69 citation statements)

References 8 publications

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“…(7)), we set up an experiment to test the validity of this dependence under conditions of high NA. We introduced a prism-based compensator [21] before the grating to vary the chirp of the input beam. The amount of chirp imparted to the beam was accomplished through translating the retroreflector as described in the reference [21].…”

Section: Resultsmentioning

confidence: 99%

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Temporally focused wide-field two-photon microscopy: Paraxial to vectorial

Yew

Sheppard

2013

Opt. Express

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Temporal focusing allows for optically sectioned wide-field microscopy. The optical sectioning arises because this method takes a pulsed input beam, stretches the pulses by diffracting off a grating, and focuses the stretched pulses such that only at the focal plane are the pulses re-compressed. This approach generates nonlinear optical processes at the focal plane and results in depth discrimination. Prior theoretical models of temporal focusing processes approximate the contributions of the different spectral components by their mean. This is valid for longer pulses that have narrower spectral bandwidth but results in a systematic deviation when broad spectrum, femtosecond pulses are used. Further, prior model takes the paraxial approximation but since these pulses are focused with high numerical aperture (NA) objectives, the effects of the vectorial nature of light should be considered. In this paper we present a paraxial and a vector theory of temporal focusing that takes into account the finite spread of the spectrum. Using paraxial theory we arrive at an analytical solution to the electric field at the focus for temporally focused wide-field two-photon (TF2p) microscopy as well as in the case of a spectrally chirped input beam. We find that using paraxial theory while accounting for the broad spectral spread gives results almost twice vector theory. Experiment results agree with predictions of the vector theory giving an axial full-width half maximum (FWHM) of 2.1μm and1.8 μm respectively as long as spectral spread is taken into account. Using our system parameters, the optical sectioning of the TF2p microscope is found to be 8 μm . The optical transfer function (OTF) of a TF2p microscope is also derived and is found to pass a significantly more limited band of axial frequencies than a point scanning two-photon (2p) microscope or a single photon (1p) confocal microscope.

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Section: Resultsmentioning

confidence: 99%

“…We introduced a prism-based compensator [21] before the grating to vary the chirp of the input beam. The amount of chirp imparted to the beam was accomplished through translating the retroreflector as described in the reference [21]. A separate line fed the beam to an autocorrelator for measuring the pulsewidth.…”

Section: Resultsmentioning

confidence: 99%

Temporally focused wide-field two-photon microscopy: Paraxial to vectorial

Yew

Sheppard

2013

Opt. Express

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“…Any optical scenario where the photons on the red end of the spectrum travel farther than those on the blue end, for the appropriate distance, would provide the necessary temporal dispersion compensation. While there are reports of using other prism schemes [56][57][58] or the spectral dispersion of the AODs themselves for this purpose, 59 we judged the present design simplest and most robust. Other dispersion compensation techniques comprised of negatively dispersive mirrors 60 or photonic crystal fibers 61,62 could be fruitful.…”

Section: Discussionmentioning

confidence: 98%

Two-photon microscope for multisite microphotolysis of caged neurotransmitters in acute brain slices

2009

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Abstract. We developed a two-photon microscope optimized for physiologically manipulating single neurons through their postsynaptic receptors. The optical layout fulfills the stringent design criteria required for high-speed, high-resolution imaging in scattering brain tissue with minimal photodamage. We detail the practical compensation of spectral and temporal dispersion inherent in fast laser beam scanning with acousto-optic deflectors, as well as a set of biological protocols for visualizing nearly diffraction-limited structures and delivering physiological synaptic stimuli. The microscope clearly resolves dendritic spines and evokes electrophysiological transients in single neurons that are similar to endogenous responses. This system enables the study of multisynaptic integration and will assist our understanding of single neuron function and dendritic computation.

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“…This 4-prism compressor setup is simplified by placing a mirror after the second prism in a 2-prism compressor setup to reflect the beam back through the 2-prism setup, or by using a retroreflector and a roof mirror to reflect the laser pulse through a prism 4 times as seen in a single-prism compressor setup. 7 We constructed a single-prism compressor ( Fig. 1) to compress the broadband white light pulse needed to do multiplex coherent anti-Stokes Raman spectroscopy (MCARS).…”

Section: Prism Compressormentioning

confidence: 99%

Compression of Ultrafast Laser Beams

Roberson¹,

Pellegrino²

2016

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Extremely simple single-prism ultrashort- pulse compressor

Cited by 93 publications

References 8 publications

Temporally focused wide-field two-photon microscopy: Paraxial to vectorial

Temporally focused wide-field two-photon microscopy: Paraxial to vectorial

Two-photon microscope for multisite microphotolysis of caged neurotransmitters in acute brain slices

Compression of Ultrafast Laser Beams

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