volume 12, issue 10, P1846-1852 2006
DOI: 10.1261/rna.128906
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Abstract: ABSTRACTIn pre-steady-state, fast-quench kinetic analysis, the tertiary-stabilized hammerhead ribozyme ''RzB'' cleaves its substrate RNA with maximal measured k obs values of ; ;3000 min À1 in 1 mM Mn 2+ and ; ;780 min À1 in 1 mM Mg 2+ at 37°C (pH 7.4). Apparent pKa for the catalytic general base is ; ;7.8-8.5, independent of the corresponding metal hydrate pKa, suggesting potential involvement of a nucleobase as general base as suggested previously from nucleobase substitution studies. The pH-rate profile is…

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