Extraction of glutathione from EFB fermentation waste using methanol with sonication process

Muryanto, Muryanto; Alvin,; Nurdin, Muhammad; Hanifah, U.; Sudiyani, Yanni

doi:10.1063/1.5011868

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…However, we found no information on its fractionation relative to dietary sulfur when it is synthesized in the body. Finally, GSH is extracted effectively with methanol, 62 the same solvent discarded during our lipid extraction protocol. If the distribution of GSH is the same in shark muscle and liver as in terrestrial mammals, this may explain the differential decrease in sulfur between the two tissues.…”

Section: Resultsmentioning

confidence: 99%

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Lipid extraction has tissue‐dependent effects on isotopic values (δ³⁴S, δ¹³C, and δ¹⁵N) from different marine predators

Riverón

Raoult

Slip

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale The use of sulfur isotopes to study trophic ecology in marine ecosystems has increased in the past decade. Unlike other commonly used isotopes (e.g., carbon), sulfur can better discriminate benthic and pelagic productivity. However, how lipid extraction affects sulfur isotopic values has not been assessed, despite its frequent use to remove lipid effects on δ13C values. Methods We used white muscle and liver samples from two species of sharks and skin samples from two species of pinnipeds (sea lion and fur seal) to assess the effects of lipid extraction on stable isotope values for δ34S, δ13C, and δ15N. Isotopic values were determined using a continuous flow‐isotope ratio mass spectrometer coupled to an elemental analyzer. Results Lipid extraction significantly decreased δ34S values in shark tissues, more so for liver than muscle (−4.6 ± 0.9‰ vs −0.8 ± 0.3‰, average change), with nearly no change in their standard deviations. Lipid extraction did not affect δ34S values from pinniped skin samples (0.2 ± 0.8‰, average change). After lipid extraction, consistent increases in δ13C values (0.2‰–7.3‰) were detected as expected, especially in tissue with high lipid content (C:N >4). After lipid extraction, significant increases in δ15N values (0.5‰–1.4‰) were found in shark muscle and liver tissues. For pinniped skin samples, δ15N values were not significantly lower after lipid extraction (−0.4‰ to –0.1‰). Conclusions Lipid extraction did not have a strong impact on δ34S values of shark muscle and pinniped skin (≤1‰). However, our results suggest it is essential to consider the effects of lipid extraction when interpreting results from δ34S values of shark liver tissue, as they significantly depleted values relative to bulk tissue (~5‰). This may reflect selective removal of sulfolipids and glutathione present in higher concentrations in the liver than in muscle and skin and requires further investigation.

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Section: Resultsmentioning

confidence: 99%

“…59,61 However, we found no information on its fractionation relative to dietary sulfur when it is synthesized in the body. Finally, GSH is extracted effectively with methanol, 62 decreases. Because of the elemental composition of GSH, the removal of GSH from the sample leads to an increase in %S relative to the total sample.…”

Section: Sulfurmentioning

confidence: 99%

Lipid extraction has tissue‐dependent effects on isotopic values (δ³⁴S, δ¹³C, and δ¹⁵N) from different marine predators

Riverón

Raoult

Slip

et al. 2022

Rapid Comm Mass Spectrometry

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“…The extraction procedure began with homogenizing the leaves in 100% methanol, ensuring effective mixing and disruption of the plant tissue. Following this, the homogenate underwent sonication for a period of 15 minutes ( Muryanto et al., 2017 ). The measurement of GSH activity was carried out using a reaction mixture comprising of 1.8 mL phosphate buffer, along with 300 μL each of EDTA, NADPH, oxidized glutathione (GSSG), and enzyme extract (1 mg mL -1 ).…”

Section: Methodsmentioning

confidence: 99%

Staphylococcus arlettae mediated defense mechanisms and metabolite modulation against arsenic stress in Helianthus annuus

Qadir,

Hussain,

Shah

et al. 2024

Front. Plant Sci.

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IntroductionArsenate, a metalloid, acting as an analog to phosphate, has a tendency to accumulate more readily in plant species, leading to adverse effects.MethodsIn the current study, sunflower seedlings were exposed to 25, 50 and 100 ppm of the arsenic.ResultsLikewise, a notable reduction (p<0.05) was observed in the relative growth rate (RGR) by 4-folds and net assimilation rate (NAR) by 75% of Helianthus annuus when subjected to arsenic (As) stress. Nevertheless, the presence of Staphylococcus arlettae, a plant growth-promoting rhizobacterium with As tolerance, yielded an escalation in the growth of H. annuus within As-contaminated media. S. arlettae facilitated the conversion of As into a form accessible to plants, thereby, increasing its uptake and subsequent accumulation in plant tissues. S. arlettae encouraged the enzymatic antioxidant systems (Superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT)) and non-enzymatic antioxidants (flavonoids, phenolics, and glutathione) in H. annuus seedlings following substantial As accumulation. The strain also induced the host plant to produce osmolytes like proline and sugars, mitigating water loss and maintaining cellular osmotic balance under As-induced stress. S. arlettae rectified imbalances in lignin content, reduced high malonaldehyde (MDA) levels, and minimized electrolyte leakage, thus counteracting the toxic impacts of the metal.ConclusionThe strain exhibited the capability to concurrently encourage plant growth and remediate Ascontaminated growth media through 2-folds rate of biotransformation and bio-mobilization.

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“…The extraction procedure began with homogenizing the leaves in 100% methanol, ensuring effective mixing and disruption of the plant tissue. Following this, the homogenate underwent sonication for a period of 15 minutes (Muryanto et al, 2017). The measurement of GSH activity was carried out using a reaction mixture comprising of 1.8 mL phosphate buffer, along with 300 mL each of EDTA, NADPH, oxidized glutathione (GSSG), and enzyme extract (1 mg mL -1 ).…”

Section: Estimation Of Non-enzymatic Antioxidant Potentialmentioning

confidence: 99%

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Extraction of glutathione from EFB fermentation waste using methanol with sonication process

Cited by 9 publications

References 19 publications

Lipid extraction has tissue‐dependent effects on isotopic values (δ³⁴S, δ¹³C, and δ¹⁵N) from different marine predators

Lipid extraction has tissue‐dependent effects on isotopic values (δ³⁴S, δ¹³C, and δ¹⁵N) from different marine predators

Staphylococcus arlettae mediated defense mechanisms and metabolite modulation against arsenic stress in Helianthus annuus

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Extraction of glutathione from EFB fermentation waste using methanol with sonication process

Cited by 9 publications

References 19 publications

Lipid extraction has tissue‐dependent effects on isotopic values (δ34S, δ13C, and δ15N) from different marine predators

Lipid extraction has tissue‐dependent effects on isotopic values (δ34S, δ13C, and δ15N) from different marine predators

Staphylococcus arlettae mediated defense mechanisms and metabolite modulation against arsenic stress in Helianthus annuus

DataSheet_1.docx

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Lipid extraction has tissue‐dependent effects on isotopic values (δ³⁴S, δ¹³C, and δ¹⁵N) from different marine predators

Lipid extraction has tissue‐dependent effects on isotopic values (δ³⁴S, δ¹³C, and δ¹⁵N) from different marine predators