Extraction of Bioactive Compounds from Haskap Leaves (Lonicera caerulea) Using Salt/Ethanol Aqueous Two-Phase Flotation

Chong, Kar Yeen; Stefanova, Roumiana; Zhang, Junzeng; Brooks, Marianne Su-Ling

doi:10.1007/s11947-020-02553-3

Cited by 16 publications

(3 citation statements)

References 60 publications

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“…Compared the recovery at the optimized salt concentration, the recovery of hesperidin in the phosphate system was rarely higher than that in the sulfate system, but the recovery of didymin and linarin was lower. The amount of the total flavonoids recovered in the ammonium sulfate system was also higher than that in the dipotassium hydrogen phosphate system, which was consistent with the previous study [28]. The reason might be the anion with a higher valence is better salting-out agent than that with a lower valence.…”

Section: Effects Of Separation Parameters On Aqueous Two-phase Flotationsupporting

confidence: 90%

Purification of linarin and hesperidin from Mentha haplocalyx by aqueous two‐phase flotation coupled with preparative HPLC and evaluation of the neuroprotective effect of linarin

Zeng

et al. 2021

J of Separation Science

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The volatile oil of Mentha haplocalyx is widely used in medicine, food, and cosmetics. However, a large amount of its residue after steam extraction of volatile oil is abandoned, resulting in a waste of resources. The method of aqueous twophase flotation coupled with preparative high-performance liquid chromatography was established for the separation and purification of nonvolatile active compounds from Mentha haplocalyx for the first time. The parameters of the twophase aqueous flotation were optimized. Under the optimal conditions including flotation solvent PEG 1000 aqueous solution (1:1, w/w), pH 5, (NH 4 ) 2 SO 4 concentration of 350 g/L in aqueous phase, N 2 flow rate of 20 mL/min, and flotation time of 20 min, the flotation efficiency of linarin, hesperidin, and didymin was 82.24, 76.38, and 89.33%, respectively. The linarin and hesperidin with the high purities of 95.8 and 97.2%, respectively, were obtained by using preparative high performance liquid chromatography. The neuroprotective effect of linarin against H 2 O 2 -induced oxidative stress in rat hippocampal neurons was investigated. The experimental result indicated that linarin could alleviate H 2 O 2 -induced oxidative stress. The work indicated that the combination of aqueous two-phase flotation and preparative high performance liquid chromatography is a feasible and practical method for the purification of nonvolatile active substances from Mentha haplocalyx, which would provide a reference process for the comprehensive utilization of M. haplocalyx. Especially, linarin might be used as a good source of natural neuroprotectants.

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Section: Effects Of Separation Parameters On Aqueous Two-phase Flotationsupporting

confidence: 90%

Purification of linarin and hesperidin from Mentha haplocalyx by aqueous two‐phase flotation coupled with preparative HPLC and evaluation of the neuroprotective effect of linarin

Zeng

et al. 2021

J of Separation Science

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“…The leaf extract prepared by ultrasonication in distilled water at 30 °C for 60 min contained several phenolic acids, such as loganic acid with anti-inflammatory properties acting against hyaluronidase and hypolipidemic potential acting against lipase, chlorogenic acid with antidiabetic potential acting against α-glucosidase, and caffeic acid with antioxidant and anti-inflammatory activity [ 83 ]. An extract prepared by aqueous two-phase flotation using ammonium sulfate-ethanol presented increased yields of flavonoids, such as rutin, luteolin-7-O-glucoside, and diosmin, which provided increased antioxidant capacity [ 84 , 85 ]. Vitamin C was also determined in the aqueous extract of haskap leaves prepared at the Soxhlet equipment operated at 105 °C, for 20 h, but at a lower value (5.1 mg/100 g) than in fruit extract (6.9 mg/100 g) and polysaccharides (7%), together with flavones, flavanones, and tanning compounds, which could provide antibacterial and antiviral activity [ 86 ].…”

Section: Bioactive Compounds Of Lonicera Caerulea ...mentioning

confidence: 99%

Health Benefits of Antioxidant Bioactive Compounds in the Fruits and Leaves of Lonicera caerulea L. and Aronia melanocarpa (Michx.) Elliot

et al. 2023

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Lonicera caerulaea L. and Aronia melanocarpa (Michx.) Elliot fruits are frequently used for their health benefits as they are rich in bioactive compounds. They are recognized as a source of natural and valuable phytonutrients, which makes them a superfood. L. caerulea presents antioxidant activity three to five times higher than other berries which are more commonly consumed, such as blackberries or strawberries. In addition, their ascorbic acid level is the highest among fruits. The species A. melanocarpa is considered one of the richest known sources of antioxidants, surpassing currants, cranberries, blueberries, elderberries, and gooseberries, and contains one of the highest amounts of sorbitol. The non-edible leaves of genus Aronia became more extensively analyzed as a byproduct or waste material due to their high polyphenol, flavonoid, and phenolic acid content, along with a small amount of anthocyanins, which are used as ingredients in nutraceuticals, herbal teas, bio-cosmetics, cosmeceuticals, food and by the pharmaceutical industry. These plants are a rich source of vitamins, tocopherols, folic acid, and carotenoids. However, they remain outside of mainstream fruit consumption, being well known only to a small audience. This review aims to shed light on L. caerulaea and A. melanocarpa and their bioactive compounds as healthy superfoods with antioxidant, anti-inflammatory, antitumor, antimicrobial, and anti-diabetic effects, and hepato-, cardio-, and neuro-protective potential. In this view, we hope to promote their cultivation and processing, increase their commercial availability, and also highlight the ability of these species to be used as potential nutraceutical sources, helpful for human health.

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“…ATPS are formed when two polymer‐salt, alcohol‐salt, and polymer‐ionic liquids, the salt‐ionic liquid (Li et al ., 2017) and alcohol or organic solvents‐ionic liquid (Li et al ., 2016) are mixed at a particular concentration, leading to the formation of a biphasic system, and they have been applied to the separation and purification of biomolecules such as proteins (Merchuck et al ., 1998), enzymes (Ooi et al ., 2009), amino acids (Wysoczanska & Macedo, 2016), chlorogenic acids and flavonoids (Chong et al ., 2020a, 2020b). In recent years, ATPS formed by ethanol and salts have been used for the partitioning of anthocyanins from sweet potatoes (Liu et al ., 2013; Yin et al ., 2017), grape juice (Wu et al ., 2014), Vaccinium uliginosium (Hua et al ., 2013), mulberry (Wu et al ., 2011; Liang et al ., 2012), Syzygium cumini fruits (Caldeira et al ., 2019) and Peristrophe bivalvis (L.) Merr .…”

Section: Introductionmentioning

confidence: 99%

Aqueous two‐phase anthocyanins partitioning from açai – laboratory scale‐up

de Abreu Siqueira,

Leite,

Braga

et al. 2023

Int J of Food Sci Tech

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SummaryAçai, a fruit typical of Amazonia in Brazil, is consumed due to beneficial health effects. Aqueous two‐phase systems (ATPS) based on ethanol (28% w/w) and ammonium sulfate (17.5% w/w) were employed in the partitioning of anthocyanins from açai. Experiments, applying 50 mL equilibrium cells and a 1 L glass vessel reactor, evaluated anthocyanin partition feasibility on a laboratory scale‐up. The anthocyanin preferentially moved to the top (ethanol‐rich phase) and sugars to the bottom (ammonium sulfate‐rich phase). Scale‐up effects were compared with the equilibrium cell experiments with anthocyanin extraction yielding more than 64% w/w, the yield of sugars in the bottom phase (> 61% w/w) and removal of sugars (97%), showing the feasibility for the scale‐up process. The anthocyanin profile from açai, ATPS partition on the bench scale, and laboratory scale‐up evaluated by UPLC‐QTOF‐MS identified cyanidin, peonidin, and pelargonidin derivatives.

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Extraction of Bioactive Compounds from Haskap Leaves (Lonicera caerulea) Using Salt/Ethanol Aqueous Two-Phase Flotation

Cited by 16 publications

References 60 publications

Purification of linarin and hesperidin from Mentha haplocalyx by aqueous two‐phase flotation coupled with preparative HPLC and evaluation of the neuroprotective effect of linarin

Purification of linarin and hesperidin from Mentha haplocalyx by aqueous two‐phase flotation coupled with preparative HPLC and evaluation of the neuroprotective effect of linarin

Health Benefits of Antioxidant Bioactive Compounds in the Fruits and Leaves of Lonicera caerulea L. and Aronia melanocarpa (Michx.) Elliot

Aqueous two‐phase anthocyanins partitioning from açai – laboratory scale‐up

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