Extraction of Amino Acids and Peptides by Reversed Micelles

Furusaki, Shintaro; Kishi, Kazuyuki

doi:10.1016/b978-0-444-88677-4.50107-0

Cited by 4 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are only a few partitioning studies for amino acids and peptides with AOT solutions. The values obtained here are compared with those reported by Furusaki and Kishi (1990) and Leodidis and Hatton (1990a,b) in Table 5. Although the experimental conditions and analytical methods are different, the values are comparable to those reported by Furusaki and Kishi (1990).…”

Section: Effect Of Feed Solutionsupporting

confidence: 57%

“…The values obtained here are compared with those reported by Furusaki and Kishi (1990) and Leodidis and Hatton (1990a,b) in Table 5. Although the experimental conditions and analytical methods are different, the values are comparable to those reported by Furusaki and Kishi (1990). The high values obtained by Leodidis and Hatton (1990a,b) could be due to the high concentrations of solutes (compared to those used in our study).…”

Section: Effect Of Feed Solutionsupporting

confidence: 57%

“…The values were not reported for Trp-Leu and Phe-Leu in their studies. The values listed for other peptides, for example, Trp-Gly, Phe-Gly, and so forth (Furusaki and Kishi, 1990), are in the range 10-15.…”

Section: Effect Of Feed Solutionmentioning

confidence: 99%

See 2 more Smart Citations

Extraction Equilibria of Amino Acids and Dipeptides in Various Organic Solutions

Hossain¹,

Fenton²

1999

J. Chem. Eng. Data

View full text Add to dashboard Cite

Experimental data on the partitioning of amino acids (tryptophan, phenylalanine) and dipeptides (tryptophan−leucine, phenylalanine−leucine) between the aqueous feed phase and the organic solvent phase are presented. The organic solvent phase consists of a carrier, sodium di-2-ethylhexyl sulfosuccinate/quaternary ammonium salt/dioleyl phosphoric acid, dissolved in (Z)-9-octadecen-1-ol (an organic solvent). The equilibrium distribution coefficient for extractions is determined by varying the following experimental conditions: feed solution pH, initial feed phase concentration, and composition of organic phase. The partition coefficient for the stripping process is also determined by varying the stripping agent (sodium hydroxide, sodium chloride, sodium carbonate), the concentration, and the pH of the stripping solution. The extraction and stripping reaction coefficients are calculated by combining the experimental data and the kinetic expressions for interfacial reactions. The variation of the values of the coefficients with the solute type (i.e. amino acid or dipeptide) and with the operating conditions of the system di-2-ethylhexyl sulfosuccinate in (Z)-9-octadecen-1-ol is presented.

show abstract

Section: Effect Of Feed Solutionsupporting

confidence: 57%

Section: Effect Of Feed Solutionsupporting

confidence: 57%

See 1 more Smart Citation

Extraction Equilibria of Amino Acids and Dipeptides in Various Organic Solutions

Hossain¹,

Fenton²

1999

J. Chem. Eng. Data

View full text Add to dashboard Cite

show abstract

“…To date, the reverse micelle system has been used for the separation of proteins (Goklen and Hatton, 1987;Aires-Barros and Cabral, 1991;Su and Lee, 1999;Noh and Imm, 2005;Hecth and Peled, 2006;Shen and Yu, 2007), peptides (Kishi and Furusaki, 1992), amino acids Furusaki and Kishi, 1992;Adachi et al, 2000;Ddvyap et aI., 2006), bovine serum albumin (BSA) (Zhang et al, 1999), enzymes (Moreno-Hagelsieb et al, 1999;Liu et al, 2004;Debnath et al, 2007) and metals (Nakashio et al, 1992;Ismael and Tondre, 1992;Zhang et aI., 2006;Majumdar and Mahapatra, 2007) as well as in a bioreactor to carry out biotransformation (Kamiya et al, 1995). Thus, reverse micelle has been demonstrated to be capable of hosting large quantities of biomolecules without causing denaturation by adjusting operational parameters (Hatton, 1985;Paradkar and Dordick, 1994;Goto et al, 1998;Cardoso et al, 1999;Shen and Yu, 2007).…”

Section: Hydrophobic 'Tail'mentioning

confidence: 99%

Reverse Micelle Liquid-Liquid Extraction of a Pharmaceutical Product

Mohd‐Setapar

Mohamad-Aziz

Harun

et al. 2012

AMR

View full text Add to dashboard Cite

Reverse micelle extraction has received considerable attention in recent years due to its ability to selectively solubilise solutes from an aqueous phase, and in the case of biomolecules to maintain their biological activities. The apparent success of research on protein extraction from the aqueous phase using reverse micelle provides motivation to study the solubilisation of antibiotic. The objective of this study is to investigate the extraction of antibiotic (penicillin G is chosen as model antibiotic) from aqueous solution (forward extraction) and from the reverse micelle to a new aqueous solution (backward extraction). Sodium di(2-ethylhexyl)sulfosuccinate (AOT) is chosen as the surfactant and isooctane as the organic solvent. The UV-Vis spectrophotometer is used to determine the mass of penicillin G in solution after the extraction process. The extraction is expected to be influenced by the initial penicillin G concentration, the salt type and concentration in the aqueous phase, pH, and surfactant concentration. It is expected that as penicillin is an interfacially active compound that will interacts with AOT surfactant, the interfacial association will be dependent on both pH and surfactant concentration.

show abstract