e present work explores membrane-mediated extraction and transport studies of amino acids through arti�cial bulk liquid membrane system with krypto�x 5 as a carrier. e various reaction parameters such as amino acid concentration, carrier concentration, time, pH, and stirring effect were studied to optimize reaction conditions. e stirring of source and receiving phases increased the efficiency of extraction process. �oncyclic receptor krypto�x 5 with �ve oxyethylene units and terminal aromatic donor end groups governs its transport and extraction efficiency. e extraction and transport efficiency followed the following trend: valine > alanine > glycine > threonine. Supported liquid membrane (SLM) studies were performed using cellulose nitrate, PTFE, eggshell, and onion membranes. e egg shell membrane support proved to be most efficient due to intricate network of water insoluble proteins �bers with very high surface area and homogeneity.