“…These compounds were mainly found in fish oil because the fishes bioaccumulated them through the consumption of phytoplankton including microalgae in the food chain, making fish an important human nutritional source [78]. However, there is now a significant challenge associated with the sustainability of fish source; fortunately, some microalgae have been reported to contain most of these critical DHAs and EPAs in abundance and could be cultivated autotrophically for economic use [79]. This study, however, found only T. reginae to contain EPA isomers (11,14,17-eicosatrienoic acid, (Z,Z,Z)-and 11,14,17-eicosatrienoic acid, methyl esters) with an appreciable percentage abundance of 10.04 ± 0.46 and 10.62 ± 0.10 and DHA (4,7,10,13,16,19-docosahexaenoic acid, methyl ester) with a low % abundance of 0.77 ± 0.07.…”