Extracellular vesicles: small bricks for tissue repair/regeneration

Taverna, Simona; Pucci, Marzia; Alessandro, Riccardo

doi:10.21037/atm.2017.01.53

Cited by 47 publications

(48 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All this may reflect the deregulation of physiological functions exerted by EV-mediated intercellular communication during organogenesis and tissue regeneration/repair [166][167][168]. In fact, EV-mediated interactions between inflammatory cells, endothelial cells, mesenchymal cells and tissue-specific stem cells are involved in the regeneration of injured tissues and organs, including skeletal muscle, cardiac muscle, gastrointestinal, neural, renal, and respiratory tissues [169][170][171][172][173].…”

Section: Evs In Tumorigenesismentioning

confidence: 99%

Extracellular Vesicles as Signaling Mediators and Disease Biomarkers across Biological Barriers

Simeone

Bologna

Lanuti

et al. 2020

IJMS

138

117

View full text Add to dashboard Cite

Extracellular vesicles act as shuttle vectors or signal transducers that can deliver specific biological information and have progressively emerged as key regulators of organized communities of cells within multicellular organisms in health and disease. Here, we survey the evolutionary origin, general characteristics, and biological significance of extracellular vesicles as mediators of intercellular signaling, discuss the various subtypes of extracellular vesicles thus far described and the principal methodological approaches to their study, and review the role of extracellular vesicles in tumorigenesis, immunity, non-synaptic neural communication, vascular-neural communication through the blood-brain barrier, renal pathophysiology, and embryo-fetal/maternal communication through the placenta.

show abstract

Section: Evs In Tumorigenesismentioning

confidence: 99%

Extracellular Vesicles as Signaling Mediators and Disease Biomarkers across Biological Barriers

Simeone

Bologna

Lanuti

et al. 2020

IJMS

138

117

View full text Add to dashboard Cite

show abstract

“…The main function ascribed to MVs is a role in intercellular communication; however, many specific functions have also been associated with them depending on their cellular origin. Cell adhesion and migration [7,8], waste management [9], vascular function [10], coagulation [11], reticulocyte maturation [12], modulation of the immune response [13], fertilization [14], embryonic development [15], bone calcification [16] and tissue repair [17,18] are activities where MVs, as well as exosomes, have been involved. Moreover, an increase of MV production has been associated with pathological states [19,20], tumor growth, metastasis and angiogenesis [21,22].…”

Section: Microvesicles (Mvs)mentioning

confidence: 99%

Microvesicles: ROS scavengers and ROS producers

Bodega

Alique

Puebla

et al. 2019

J of Extracellular Vesicle

181

151

View full text Add to dashboard Cite

This review analyzes the relationship between microvesicles and reactive oxygen species (ROS). This relationship is bidirectional; on the one hand, the number and content of microvesicles produced by the cells are affected by oxidative stress conditions; on the other hand, microvesicles can directly and/or indirectly modify the ROS content in the extra- as well as the intracellular compartments. In this regard, microvesicles contain a pro-oxidant or antioxidant machinery that may produce or scavenge ROS: direct effect. This mechanism is especially suitable for eliminating ROS in the extracellular compartment. Endothelial microvesicles, in particular, contain a specific and well-developed antioxidant machinery. On the other hand, the molecules included in microvesicles can modify (activate or inhibit) ROS metabolism in their target cells: indirect effect. This can be achieved by the incorporation into the cells of ROS metabolic enzymes included in the microvesicles, or by the regulation of signaling pathways involved in ROS metabolism. Proteins, as well as miRNAs, are involved in this last effect.

show abstract

“…EVs can be isolated from cell culture medium and various body fluids, including plasma, CSF and urine [ 22–24 ]. The delivery of EVs from one cell to another is known to constitute an important form of intercellular communication, distributing proteins, lipids, nucleic acids, and sugar molecules [ 25 ]. However, the role of EVs in AD progression and the material that EVs carry in response to Aβ 42 pathology is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Increased Release of Apolipoprotein E in Extracellular Vesicles Following Amyloid-β Protofibril Exposure of Neuroglial Co-Cultures

Nikitidou

Khoonsari

Shevchenko

et al. 2017

JAD

View full text Add to dashboard Cite

Extracellular vesicles (EVs), including exosomes and larger microvesicles, have been implicated to play a role in several conditions, including Alzheimer’s disease (AD). Since the EV content mirrors the intracellular environment, it could contribute with important information about ongoing pathological processes and may be a useful source for biomarkers, reflecting the disease progression. The aim of the present study was to analyze the protein content of EVs specifically released from a mixed co-culture of primary astrocytes, neurons, and oligodendrocytes treated with synthetic amyloid-β (Aβ42) protofibrils. The EV isolation was performed by ultracentrifugation and validated by transmission electron microscopy. Mass spectrometry analysis of the EV content revealed a total of 807 unique proteins, of which five displayed altered levels in Aβ42 protofibril exposed cultures. The most prominent protein was apolipoprotein E (apoE), and by western blot analysis we could confirm a threefold increase of apoE in EVs from Aβ42 protofibril exposed cells, compared to unexposed cells. Moreover, immunoprecipitation studies demonstrated that apoE was primarily situated inside the EVs, whereas immunocytochemistry indicated that the EVs most likely derived from the astrocytes and the neurons in the culture. The identified Aβ-induced sorting of apoE into EVs from cultured neuroglial cells suggests a possible role for intercellular transfer of apoE in AD pathology and encourage future studies to fully elucidate the clinical relevance of this event.

show abstract

Extracellular vesicles: small bricks for tissue repair/regeneration

Cited by 47 publications

References 30 publications

Extracellular Vesicles as Signaling Mediators and Disease Biomarkers across Biological Barriers

Extracellular Vesicles as Signaling Mediators and Disease Biomarkers across Biological Barriers

Microvesicles: ROS scavengers and ROS producers

Increased Release of Apolipoprotein E in Extracellular Vesicles Following Amyloid-β Protofibril Exposure of Neuroglial Co-Cultures

Contact Info

Product

Resources

About