2019
DOI: 10.3390/cancers11091276
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Abstract: Prostate cancer (PCa) progression is strictly associated with microenvironmental conditions, which can be modified by cancer-released extracellular vesicles (EVs), important mediators of cell-cell communication. However, the role of EVs in the inflammatory cross-talk between cancer cells and microenvironment-residing cells remains largely unknown. To evaluate the role of EVs in the tumour microenvironment, we treated the non-cancerous prostate cell line PNT2 with EVs isolated from advanced-stage prostate cance… Show more

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Cited by 18 publications
(33 citation statements)
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“…As aforementioned, the canonical inflammasome activation requires two signals: the first is represented by TLR-ligand engagement; the second, provided by stress signals, results in Caspase-1 activation. Lysosomal disruption appears to be a critical event in the second step and an increase in Cathepsin B cleaved/active form has been shown to contribute to Caspase-1 activation [38]. In order to determine the involvement of ANP, BNP, and the related cGMP/PKG-I axis in canonical inflammasome activation, we analyzed Cathepsin B cleavage in our experimental conditions ( Figure 5A).…”
Section: Cgmp But Not Pkg-i Is Involved In Non-canonical and Alternatmentioning
confidence: 99%
“…As aforementioned, the canonical inflammasome activation requires two signals: the first is represented by TLR-ligand engagement; the second, provided by stress signals, results in Caspase-1 activation. Lysosomal disruption appears to be a critical event in the second step and an increase in Cathepsin B cleaved/active form has been shown to contribute to Caspase-1 activation [38]. In order to determine the involvement of ANP, BNP, and the related cGMP/PKG-I axis in canonical inflammasome activation, we analyzed Cathepsin B cleavage in our experimental conditions ( Figure 5A).…”
Section: Cgmp But Not Pkg-i Is Involved In Non-canonical and Alternatmentioning
confidence: 99%
“…Extracellular vesicles were isolated as previously described [ 6 ]. Briefly, PC3 cells were grown in RPMI 1640 supplemented with extracellular-vesicles-depleted FBS for 3 days.…”
Section: Methodsmentioning
confidence: 99%
“…Extracellular vesicles were stained with 50 μM 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine,4-chlorobenzenesulfonate salt (DiD’; Thermofisher, Carlsbad, CA, USA) for 30 min, as previously described [ 6 ]. TPA-differentiated THP-1 cells, seeded on glass coverslips, were exposed to 100 μg/mL of DiD’-stained PC3-EVs.…”
Section: Methodsmentioning
confidence: 99%
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