Extracellular vesicles derived from hypoxic glioma stem-like cells confer temozolomide resistance on glioblastoma by delivering miR-30b-3p

Yin, Jianxing; Ge, Xin; Shi, Zhumei; Chen, Yu; Lu, Chenfei; Wei, Yutian; Zeng, Ailiang; Wang, Xiefeng; Yan, Wei; Zhang, Junxia; You, Yongping

doi:10.7150/thno.47057

Cited by 69 publications

(46 citation statements)

References 57 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Stem cell-derived EVs have been reported to contain transcription factors that are secreted by stem cells, which are responsible for maintaining stem cell properties, as well as secreted signaling molecules, such as WNTs, TGF-β, and β-catenin, implicated in EV-mediated therapeutic actions [ 75 , 76 , 77 ]. EVs can also convey RNAs, including mRNAs, miRNAs, and lncRNAs, which represent their cells of origin [ 78 , 79 , 80 , 81 , 82 , 83 , 84 ]. Interestingly, EVs possess the capacity to transfer specific proteins and genetic materials to the recipient cells and, also, EV membranes can be engineered using organ-specific therapeutic proteins or drugs for treating the incurable disease of target organs [ 85 , 86 , 87 , 88 , 89 , 90 ].…”

Section: Discussionmentioning

confidence: 99%

High Therapeutic and Esthetic Properties of Extracellular Vesicles Produced from the Stem Cells and Their Spheroids Cultured from Ocular Surgery-Derived Waste Orbicularis Oculi Muscle Tissues

et al. 2021

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are paracrine factors that mediate stem cell therapeutics. We aimed at evaluating the possible therapeutic and esthetic applications of EVs prepared from the waste human facial tissue-derived orbicularis oculi muscle stem cells (OOM-SCs). OOM-SCs were isolated from the ocular tissues (from elders and youngsters) after upper eyelid blepharoplasty or epiblepharon surgeries. EVs were prepared from the OOM-SCs (OOM-SC-EVs) and their three-dimensional spheroids. OOM-SCs showed a spindle-like morphology with trilineage differentiation capacity, positive expression of CD105, CD 90, and CD73, and negative expression of CD45 and CD34, and their stem cell properties were compared with other adult mesenchymal stem cells. OOM-SC-EVs showed a high inhibitory effect on melanin synthesis in B16F10 cells by blocking tyrosinase activity. OOM-SC-EVs treatment led to a significant attenuation of senescence-associated changes, a decrease in reactive oxygen species generation, and an upregulation of antioxidant genes. We demonstrated the regeneration activity of OOM-SC-EVs in in vitro wound healing of normal human dermal fibroblasts and upregulation of anti-wrinkle-related genes and confirmed the therapeutic potential of OOM-SC-EVs in the healing of the in vivo wound model. Our study provides promising therapeutic and esthetic applications of OOM-SC-EVs, which can be obtained from the ocular surgery-derived waste human facial tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

High Therapeutic and Esthetic Properties of Extracellular Vesicles Produced from the Stem Cells and Their Spheroids Cultured from Ocular Surgery-Derived Waste Orbicularis Oculi Muscle Tissues

et al. 2021

View full text Add to dashboard Cite

show abstract

“…And the downregulation of hsa-miR-30b-3p leads to the poor prognosis of gastric cancer [ 63 ]. Besides, miR-30b-3p affects the outcome of ovarian cancer, hepatocellular, and so on [ 64 – 66 ].…”

Section: Discussionmentioning

confidence: 99%

ITGB1-DT/ARNTL2 axis may be a novel biomarker in lung adenocarcinoma: a bioinformatics analysis and experimental validation

et al. 2021

View full text Add to dashboard Cite

Background Lung cancer is one of the most lethal malignant tumors that endangers human health. Lung adenocarcinoma (LUAD) has increased dramatically in recent decades, accounting for nearly 40% of all lung cancer cases. Increasing evidence points to the importance of the competitive endogenous RNA (ceRNA) intrinsic mechanism in various human cancers. However, behavioral characteristics of the ceRNA network in lung adenocarcinoma need further study. Methods Groups based on SLC2A1 expression were used in this study to identify associated ceRNA networks and potential prognostic markers in lung adenocarcinoma. The Cancer Genome Atlas (TCGA) database was used to obtain the patients' lncRNA, miRNA, and mRNA expression profiles, as well as clinical data. Informatics techniques were used to investigate the effect of hub genes on prognosis. The Cox regression analyses were performed to evaluate the prognostic effect of hub genes. The methylation, GSEA, and immune infiltration analyses were utilized to explore the potential mechanisms of the hub gene. The CCK-8, transwell, and colony formation assays were performed to detect the proliferation and invasion of lung cancer cells. Results We eventually identified the ITGB1-DT/ARNTL2 axis as an independent fact may promote lung adenocarcinoma progression. Furthermore, methylation analysis revealed that hypo-methylation may cause the dysregulated ITGB1-DT/ARNTL2 axis, and immune infiltration analysis revealed that the ITGB1-DT/ARNTL2 axis may affect the immune microenvironment and the progression of lung adenocarcinoma. The CCK-8, transwell, and colonu formation assays suggested that ITGB1-DT/ARNTL2 promotes the progression of lung adenocarcinoma. And hsa-miR-30b-3p reversed the ITGB1/ARNTL2-mediated oncogenic processes. Conclusion Our study identified the ITGB1-DT/ARNTL2 axis as a novel prognostic biomarker affects the prognosis of lung adenocarcinoma.

show abstract

“…Studies have found that miR-30b-3p can bind to sites on the 3′-UTR of ras homologue family member B (RHOB), decrease cell cycle arrest and inhibit cell apoptosis induced by TMZ. As a translation factor, STAT3 can bind to the miR-30b promoter, but STAT3 must form a complex with HIF1α to induce miR-30b-3p expression in GSCs under hypoxic conditions [ 73 ]. In contrast, STAT3 can inhibit the expression of some miRNAs.…”

Section: Mirnas Involved In the Stat3 Signalling Pathwaymentioning

confidence: 99%

Noncoding RNAs involved in the STAT3 pathway in glioma

Zheng

et al. 2021

Cancer Cell Int

View full text Add to dashboard Cite

Glioma is the most common malignant primary brain tumour in adults. Despite improvements in neurosurgery and radiotherapy, the prognosis of glioma patients remains poor. One of the main limitations is that there are no proper clinical therapeutic targets for glioma. Therefore, it is crucial to find one or more effective targets. Signal transducer and activator of transcription 3 (STAT3) is a member of the STAT family of genes. Abnormal expression of STAT3 is involved in the process of cell proliferation, migration, invasion, immunosuppression, angiogenesis, dryness maintenance, and resistance to radiotherapy and chemotherapy in glioma. Therefore, STAT3 has been considered an ideal therapeutic target in glioma. Noncoding RNAs (ncRNAs) are a group of genes with limited or no protein-coding capacity that can regulate gene expression at the epigenetic, transcriptional and posttranscriptional level. In this review, we summarized the ncRNAs that are correlated with the ectopic expression of STAT3 in glioma.

show abstract

Extracellular vesicles derived from hypoxic glioma stem-like cells confer temozolomide resistance on glioblastoma by delivering miR-30b-3p

Cited by 69 publications

References 57 publications

High Therapeutic and Esthetic Properties of Extracellular Vesicles Produced from the Stem Cells and Their Spheroids Cultured from Ocular Surgery-Derived Waste Orbicularis Oculi Muscle Tissues

High Therapeutic and Esthetic Properties of Extracellular Vesicles Produced from the Stem Cells and Their Spheroids Cultured from Ocular Surgery-Derived Waste Orbicularis Oculi Muscle Tissues

ITGB1-DT/ARNTL2 axis may be a novel biomarker in lung adenocarcinoma: a bioinformatics analysis and experimental validation

Noncoding RNAs involved in the STAT3 pathway in glioma

Contact Info

Product

Resources

About