Extracellular Vesicles and Host–Pathogen Interactions: A Review of Inter-Kingdom Signaling by Small Noncoding RNA

Stanton, Bruce A.

doi:10.3390/genes12071010

Cited by 36 publications

(28 citation statements)

References 80 publications

(209 reference statements)

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“…miR-155, which is involved in TLR activation via bacteria-derived lipopolysaccharides, was shown to activate tumor necrosis factor (TNF)-α and interleukin (IL)-6 and regulate suppressor of cytokine signaling 1 (SOCS1) on dendritic cells, thus playing a role in adaptive immune responses ( Esmerina et al, 2007 ; Naqvi et al, 2014 ). In addition, bacteria can secrete extracellular vesicles that carry extracellular miRNAs; these vesicles participate in intercellular communication to reach remote target cells ( Yu S. R et al, 2019 ; Badi et al, 2020 ; Stanton, 2021 ). For example, the probiotic E. coli Nissle 1917 strain produces outer-membrane vesicles that control the expression of the ZO-1 and ZO-2 tight junction proteins, enhancing intestinal immune regulation and barrier function ( Veltman et al, 2012 ; Sabharwal et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Global trends in research on miRNA–microbiome interaction from 2011 to 2021: A bibliometric analysis

Yan¹,

Yao²,

Li³

et al. 2022

Front. Pharmacol.

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An increasing number of research suggests that the microRNA (miRNA)–microbiome interaction plays an essential role in host health and diseases. This bibliometric analysis aimed to identify the status of global scientific output, research hotspots, and frontiers regarding the study of miRNA–microbiome interaction over the past decade. We retrieved miRNA–microbiome-related studies published from 2011 to 2021 from the Web of Science Core Collection database; the R package bibliometrix was used to analyze bibliometric indicators, and VOSviewer was used to visualize the field status, hotspots, and research trends of miRNA–microbiome interplay. In total, 590 articles and reviews were collected. A visual analysis of the results showed that significant increase in the number of publications over time. China produced the most papers, and the United States contributed the highest number of citations. Shanghai Jiaotong University and the University of California Davis were the most active institutions in the field. Most publications were published in the areas of biochemistry and molecular biology. Yu Aiming was the most prolific writer, as indicated by the h-index and m-index, and Liu Shirong was the most commonly co-cited author. A paper published in the International Journal of Molecular Sciences in 2017 had the highest number of citations. The keywords “expression” and “gut microbiota” appeared most frequently, and the top three groups of diseases that appeared among keywords were cancer (colorectal, et al.), inflammatory bowel disease (Crohn’s disease and ulcerative colitis), and neurological disorders (anxiety, Parkinson’s disease, et al.). This bibliometric study revealed that most studies have focused on miRNAs (e.g., miR-21, miR-155, and miR-146a), gut microbes (e.g., Escherichia coli, Bifidobacterium, and Fusobacterium nucleatum), and gut bacteria metabolites (e.g., butyric acid), which have the potential to improve the diagnosis, treatment, and prognosis of diseases. We found that therapeutic strategies targeting the miRNA–microbiome axis focus on miRNA drugs produced in vitro; however, some studies suggest that in vivo fermentation can greatly increase the stability and reduce the degradation of miRNA. Therefore, this method is worthy of further research.

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Section: Discussionmentioning

confidence: 99%

Global trends in research on miRNA–microbiome interaction from 2011 to 2021: A bibliometric analysis

Yan¹,

Yao²,

Li³

et al. 2022

Front. Pharmacol.

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“…More recently, microbial RNA and protein cargo in bEVs have been systemically delivered into host cells in an animal model [ 17 ]. Other studies have also presented evidence regarding host–microbe interkingdom transfer of extracellular RNAs via bEVs [ 18 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

Use of Bacterial Extracellular Vesicles for Gene Delivery to Host Cells

Kim

Choi

et al. 2022

Biomolecules

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Extracellular vesicles (EVs), which are nanosized membranous particles secreted from both prokaryotic and eukaryotic cells, can deliver various biological molecules, such as nucleic acids, proteins, and lipids, into recipient cells. However, contrary to what is known about eukaryotic EVs, whether bacterial EVs (bEVs) can be used as transporters for bioactive molecules is becoming a hot area of research. In this study, we electroporated enhanced green fluorescent protein (EGFP) genes and precursor microRNA of Cel-miR-39 (pre-Cel-miR-39) from isolated bEVs of Escherichia coli and Lactobacillus reuteri. The EGFP plasmid, synthetic EGFP RNA, and pre-Cel-miR-39 were successfully delivered into the murine microglial BV2 cells via bEVs. PCR and confocal microscopy analysis confirmed the transfer of the EGFP plasmid and RNA. The bEV-delivered exogenous pre-Cel-miR-39 was further processed into the mature form of Cel-miR-39; its incorporation into Ago2—a major component of the RNA-induced silencing complex—was assessed using RNA-immunoprecipitation–PCR. Taken together, bEVs can be used as vehicles to deliver genetic materials and for novel biotechnological applications, such as gene transfer and mRNA vaccines.

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“…Moreover, intracellular pathogens modulate their own and host miRNAs that participate in cellular processes relevant to pathogen replication and promotion of its life cycle [140]. Particularly interesting are miRNAs delivered by exovesicles since they are mediators of Inter-Kingdom communication between host cells and pathogens [141,142].…”

Section: Micrornas In the Host-parasite Interactionmentioning

confidence: 99%

MicroRNAs: master regulators in host–parasitic protist interactions

et al. 2022

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MicroRNAs (miRNAs) are a group of small non-coding RNAs present in a wide diversity of organisms. MiRNAs regulate gene expression at a post-transcriptional level through their interaction with the 3′ untranslated regions of target mRNAs, inducing translational inhibition or mRNA destabilization and degradation. Thus, miRNAs regulate key biological processes, such as cell death, signal transduction, development, cellular proliferation and differentiation. The dysregulation of miRNAs biogenesis and function is related to the pathogenesis of diseases, including parasite infection. Moreover, during host–parasite interactions, parasites and host miRNAs determine the probability of infection and progression of the disease. The present review is focused on the possible role of miRNAs in the pathogenesis of diseases of clinical interest caused by parasitic protists. In addition, the potential role of miRNAs as targets for the design of drugs and diagnostic and prognostic markers of parasitic diseases is also discussed.

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Extracellular Vesicles and Host–Pathogen Interactions: A Review of Inter-Kingdom Signaling by Small Noncoding RNA

Cited by 36 publications

References 80 publications

Global trends in research on miRNA–microbiome interaction from 2011 to 2021: A bibliometric analysis

Global trends in research on miRNA–microbiome interaction from 2011 to 2021: A bibliometric analysis

Use of Bacterial Extracellular Vesicles for Gene Delivery to Host Cells

MicroRNAs: master regulators in host–parasitic protist interactions

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