Extracellular matrix proteins in healthy and retained placentas, comparing hemochorial and synepitheliochorial placentas

Franczyk, Monika; Łopucki, Maciej; Stachowicz, Norbert; Morawska, D.; Kankofer, Marta

doi:10.1016/j.placenta.2016.12.014

Cited by 18 publications

(13 citation statements)

References 64 publications

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“…EM is composed of proteoglycans, glycosaminoglycans and has collagen as its main structural component. Most placental collagen is type III (around 60%), followed by type I collagen (approximately 30%) and the other types are IV, V, and VI ( 43 , 44 ). MMPs play a role in the implantation, vasodilatation and separation of fetal membranes, developing a crucial role in collagen degradation according to the signaling by hormones ( 45 , 46 ).…”

Section: Discussionmentioning

confidence: 99%

Zika Induces Human Placental Damage and Inflammation

et al. 2020

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In Brazil, an epidemic of Zika virus (ZIKV) infections was declared in 2015 that coincided with alarming reports of microcephaly in newborns associated with mother infection. Although the virus has placental tropism, changes in the tissue morphology and immunity of infected patients have not yet been elucidated. Here, we investigated the histopathological and ultrastructural changes along with the immunological profile and the BDNF expression in rare placental material. Tissues were obtained in the 2015–2016 Brazilian epidemic, of ten ZIKV-infected patients during pregnancy, five resulting in cases of fetal microcephaly and five non-microcephaly, compared to five non-infected control placentae. Viral antigens were only detected in samples from the ZIKV infected patients. Infected placentae presented histopathological severe damage, while the ultrastructural evaluation showed abnormal organelles, such as clusters of virus-like particles consistent with the ZIKV dimensions. Increased infiltration of CD68 + and TCD8 + cells, expression of MMPs, cytokines (IFN-γ and TNF-α) and other immunological mediators (RANTES/CCL5 and VEGFR-2) confirmed excessive inflammation and vascular permeability dysfunction. An evaluation of BDNF showed a decrease that could modulate neuronal damage in the developing fetus. The placental changes caused by ZIKV are not pathognomonic, however, the data provide evidence that this infection leads to severe placental injury.

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Section: Discussionmentioning

confidence: 99%

Zika Induces Human Placental Damage and Inflammation

et al. 2020

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“…The main function of decorin seems to be the involvement in ECM organization, including the interaction and regulation of collagen self-assembly. DCN is involved the formation of collagen fibers during fibrillogenesis causing changes in its properties [ 7 ]. The highest binding affinity of bovine decorin has been observed to type III, I and V collagen respectively [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Preliminary results of the placental decorin profile in bovine pregnancy and parturition

2018

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Decorin is a small leucine-rich proteoglycan which is involved in multiple biological functions mainly as a structural and signaling molecule. Due to its biological properties in connective tissue, decorin may participate in remodeling of ECM during attachment and detachment of placenta within the course of pregnancy and at parturition in cows. The aim of the study was to detect the presence of decorin protein in bovine placental tissues and to evaluate its profile during pregnancy and at parturition. Placental tissues from healthy pregnant cows (2–5 month) were collected in abattoir (n = 10), while parturient tissues were obtained during caesarian section at physiological term (n = 6). Maternal and fetal parts were separated manually and subjected to homogenization and to quantitative ELISA and verification by Western blotting with anti-decorin antibodies. ELISA test showed that the concentration of decorin during pregnancy was higher in the fetal part of placenta compared with the maternal part (p < 0.001). Similar pattern was noted regarding to maternal and fetal samples derived from parturient cows. Our preliminary results demonstrate that the concentration of decorin is gestation time-dependent in healthy bovine placenta. Possible confirmation of the involvement of decorin in early pregnancy attachment and detachment of the placenta during parturition requires further research.

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“…Few investigations on proteomic differences between retained and normal placenta were carried out so far. A review describing the involvement of extracellular matrix proteins in placenta release was recently published [45]. The 2-DGE reference map for bovine placenta during late pregnancy identified 273 proteins, providing the background for studies on molecular mechanism of placenta modification and diseases during late pregnancy [46].…”

Section: Metritis and Endometritismentioning

confidence: 99%

Proteomics and metabolomics characterizing the pathophysiology of adaptive reactions to the metabolic challenges during the transition from late pregnancy to early lactation in dairy cows

Ceciliani

Lecchi

Urh

et al. 2018

Journal of Proteomics

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For high-yielding dairy cows there are several "occupational diseases" that occur mainly during the metabolic challenges related to the transition from pregnancy to lactation. Such diseases and their sequelae form a major concern for dairy production, and often lead to early culling of animals. Beside the economical perspective, metabolic stress may severely influence animal welfare. There is a multitude of studies about the metabolic backgrounds of such so called production diseases like ketosis, fatty liver, or hypocalcaemia, although the investigations aiming to assess the complexity of the pathophysiological reactions are largely focused on gene expression, i.e. transcriptomics. For extending the knowledge towards the proteome and the metabolome, the respective technologies are of increasing importance and can provide an overall view of how dairy cows react to metabolic stress, which is needed for an in-depth understanding of the molecular mechanisms of the related diseases. We herein review the current findings from studies applying proteomics and metabolomics to transition-related diseases, including fatty liver, ketosis, endometritis, hypocalcaemia and laminitis. For each disease, a brief overview of the up to date knowledge about its pathogenesis is provided, followed by an insight into the most recent achievements on the proteome and metabolome of tissues and biological fluids, such as blood serum and urine, highlighting potential biomarkers. We believe that this review would help readers to be become more familiar with the recent progresses of molecular background of transition-related diseases thus encouraging research in this field.

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Extracellular matrix proteins in healthy and retained placentas, comparing hemochorial and synepitheliochorial placentas

Cited by 18 publications

References 64 publications

Zika Induces Human Placental Damage and Inflammation

Zika Induces Human Placental Damage and Inflammation

Preliminary results of the placental decorin profile in bovine pregnancy and parturition

Proteomics and metabolomics characterizing the pathophysiology of adaptive reactions to the metabolic challenges during the transition from late pregnancy to early lactation in dairy cows

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