Extracellular matrix–inspired gelatin/hyaluronic acid injectable hydrogels

Sanmartín-Masiá, Esther; Poveda-Reyes, Sara; Ferrer, Gloria Gallego

doi:10.1080/00914037.2016.1201828

Cited by 39 publications

(28 citation statements)

References 46 publications

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“…Successful tyramine grafting, tyramine substitution degree, and mean molecular weight of the tyramine conjugates of Gel and HA were determined by proton nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and size exclusion chromatography, and the details can be found in our previous publications. 25 , 29 …”

Section: Resultsmentioning

confidence: 99%

Hybrid Protein–Glycosaminoglycan Hydrogels Promote Chondrogenic Stem Cell Differentiation

et al. 2017

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Gelatin–hyaluronic acid (Gel–HA) hybrid hydrogels have been proposed as matrices for tissue engineering because of their ability to mimic the architecture of the extracellular matrix. Our aim was to explore whether tyramine conjugates of Gel and HA, producing injectable hydrogels, are able to induce a particular phenotype of encapsulated human mesenchymal stem cells without the need for growth factors. While pure Gel allowed good cell adhesion without remarkable differentiation and pure HA triggered chondrogenic differentiation without cell spreading, the hybrids, especially those rich in HA, promoted chondrogenic differentiation as well as cell proliferation and adhesion. Secretion of chondrogenic markers such as aggrecan, SOX-9, collagen type II, and glycosaminoglycans was observed, whereas osteogenic, myogenic, and adipogenic markers (RUNX2, sarcomeric myosin, and lipoproteinlipase, respectively) were not present after 2 weeks in the growth medium. The most promising matrix for chondrogenesis seems to be a mixture containing 70% HA and 30% Gel as it is the material with the best mechanical properties from all compositions tested here, and at the same time, it provides an environment suitable for balanced cell adhesion and chondrogenic differentiation. Thus, it represents a system that has a high potential to be used as the injectable material for cartilage regeneration therapies.

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Section: Resultsmentioning

confidence: 99%

Hybrid Protein–Glycosaminoglycan Hydrogels Promote Chondrogenic Stem Cell Differentiation

et al. 2017

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“…The nanocomposite hydrogels improved mechanical properties and some biological functions as compared with the original hydrogels [21]. In fact, annealed nano-hydroxyapatite-loaded polyvinyl alcol and poly acrylic acid (or polyvinylpyrolidone) composite hydrogel exhibited good thermostability, strength and mechanical properties [22,23]. Beta-tricalcium phosphatedispersed hydrogels significantly improved the compressive strength and biomineralization in simulated body fluid.…”

Section: Introductionmentioning

confidence: 97%

in situ Fabrication of Biological Chitosan and Gelatin-Based Hydrogels Loading Biphasic Calcium Phosphate Nanoparticles for Bone Tissue Regeneration

Nguyen

Huynh

et al. 2019

Asian J. Chem.

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Adjustably biodegradable materials have gained much attention in biomedical applications. Among of them, various hydrogel-based scaffolds have applied for regenerating soft and hard tissues. In this study, according to differently biological properties of gelatin or chitosan as well as biphasic calcium phosphate nanoparticles (BCPNPs), several injectable nanocomposite hydrogels (INgel) were enzymatically fabricated from a phenolic chitosan derivative (PCD), phenolic gelatin derivative (PGD) and BCPNPs. According the change of H2O2 concentration with follow-up the time, the in situ formation of INgel was varied from 35 to 80 s. The degradation rate of the nanocomposite materials significantly related to in presence of collagenase that expended from 3 days to over one month depending on amount of the formulated PCD. The BCPNPs-encapsulated PCD-PGD INgel enhanced mineralization in the simulated biofluid. Fluorescent cytotoxicity assay indicated that the INgel was fabricated from a higher amount of the PGD resulting in a significant proliferation of bone marrow mesenchymal stem cells. These preliminary results exhibited a great potential of the INgel for bone regeneration.

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“…According to the properties, for example the ECM of bone tissue has to present excellent stiffness and toughness, which is achieved by the incorporation of important amount of mineral components (hydroxyapatite) , whereas in the composition of the vascular system (blood vessels) and ligaments in joints, the elastin in conjugation with collagen are fundamental to bring the adequate elasticity required. [1][2][3][4][5][6][7][8][9][10][11][12] The scheme of Fig This tunability is fairly demonstrated in the work of Chan et al where the UV intensity, PI concentration and UV exposure modulation considerably affects the microparticle size and stiffness [17]. Moreover, photocrosslinking is a low-cost technique that can be carried out at ambient conditions [5,6,11,12,14,[17][18][19][20][21][22].…”

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confidence: 98%

“…In addition, enzymes possess high selectivity and energy-efficiency. Although several kinds of enzymes have been used for the crosslinking of hydrogels, if we focus on gelatin and hyaluronic acid networks, horseradish peroxidase (HRP)[8,[61][62][63][64] and transglutaminase (MTGase) are the most commonly used ones[62,65].After the work of Kurisawa et al[66], the HPR-mediated crosslinking process has been extensively studied. For this protocol, it is necessary the presence of phenolic groups, which has been boarded through the functionalization of Gel and HA with tyramine (Tyr) residues, and hydrogen peroxide (H 2 O 2 ) as oxidant substrate[61,63].For example, Sanmartin-Masia et al obtained Gel-Tyr and HA-Tyr derivatives through the reaction of carboxylic groups of Gel and HA with amine groups of Tyramine [8].…”

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confidence: 99%

Contribution of bioactive hyaluronic acid and gelatin to regenerative medicine. Methodologies of gels preparation and advanced applications

Mora‐Boza

Puertas-Bartolomé

Vázquez

et al. 2017

European Polymer Journal

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Extracellular matrix–inspired gelatin/hyaluronic acid injectable hydrogels

Cited by 39 publications

References 46 publications

Hybrid Protein–Glycosaminoglycan Hydrogels Promote Chondrogenic Stem Cell Differentiation

Hybrid Protein–Glycosaminoglycan Hydrogels Promote Chondrogenic Stem Cell Differentiation

in situ Fabrication of Biological Chitosan and Gelatin-Based Hydrogels Loading Biphasic Calcium Phosphate Nanoparticles for Bone Tissue Regeneration

Contribution of bioactive hyaluronic acid and gelatin to regenerative medicine. Methodologies of gels preparation and advanced applications

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