Extracellular L-Glutaminase Production By Marine Brevundimonas Diminuta MTCC 8486

Jayabalan, R.; Jeeva, Subbiah; Inbakandan, D.; Swaminathan, K.

doi:10.18000/ijabeg.10065

Cited by 5 publications

(1 citation statement)

References 13 publications

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“…Among these sources ammonium nitrate was showed best in-organic nitrogen and it produce 10.30 U/ml and ammonium sulphate showed 3.04 U/ml. In the study of Jayabalan et al, [19] state that, among the inorganic nitrogen and salt sources tested, only potassium dihydrogen phosphate was found to enhance the L-glutaminase production (44.3 U/ml). Ammonium sulphate, sodium nitrate and calcium nitrate were found to decrease the enzyme production at 1%, w/v concentration, but sodium nitrate evolved better in-organic nitrogen source by using B. diminuta and it showed 41.2 ±0.5U/ml.…”

Section: Resultsmentioning

confidence: 97%

Role of Nitrogen Source for L-Glutaminase Production from Fungal Strain using through Submerged Fermentation

et al. 2024

JAZ

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L-glutaminase has attracted much attention due its wide range of applications in several fields. The L-glutaminase widely used in pharmaceutical and food industries. L-glutaminase is generally regarded as a key enzyme that controls the delicious taste of fermented foods such as soy sauce. L-glutaminase production was carried out by using supplementation of organic and inorganic nitrogen sources such as yeast extract, malt extract, peptone and urea at concentration ranging from 0.25% to 1.25% with increments of 0.25% and also different inorganic nitrogen sources like ammonium sulphate and ammonium chloride at concentration ranging from 0.025% to 0.125% with increments of 0.025%. The malt extract (1%) produced 399.9 IU, were best organic nitrogen source and ammonium sulphate (0.1%) appear to be good inorganic nitrogen source under submerged fermentation process and showed 546 IU. Current study is an exploring step to industrial sector to upscale their L-glutaminase production and it will useful strategy to commercial sector and alternative to old methods.

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Section: Resultsmentioning

confidence: 97%

Role of Nitrogen Source for L-Glutaminase Production from Fungal Strain using through Submerged Fermentation

et al. 2024

JAZ

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Statistical monitoring of a web server for error rates: a bivariate time-series copula-based modeling approach

Ara

Louzada

Diniz

2017

Journal of Applied Statistics

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Optimization of Process Parameters for the Production of L-Glutaminase With Mixed Substrate by Solid State Fermentation Using Aspergillus Wentii MTCC 1901

Sameera¹,

Raju²

2015

IJRET

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L-Glutaminase, an amidohydrolase enzyme has been a choice of interest in the treatment of leukaemia since the discovery of its anti-tumour properties. Because it is a potent anti leukemic agent and a flavor-enhancing agent used in the food industry, many researchers have focused their attention on L-glutaminase. L-Glutaminase is majorly produced by micro organisms including bacteria, yeast and fungi. In the present study, production of L-Glutaminase from fungi, Aspergillus wentii was reported. Solid state fermentation was followed in the study for the enzyme production using different agro-industrial by products which include coconut oil cake, sesame oil cake, groundnut oil cake and neem oil cake. Out of them potential substrates were screened and used as mixed substrate. Mixed substrate was selected by mixing coconut oil cake and sesame oil cake in different compositions. The best composition, 1.25gm coconut oil cake and 3.75gm sesame oil cake was selected. Effect of process parameters namely temperature, pH, incubation time, moisture content, inoculum volume on enzyme production was investigated. Also effect of supplementary carbon sources, nitrogen sources, metal ions and glutamine concentration was studied and their optimum conditions were determined. The organism produced high levels of enzyme at an optimum temperature of 28C and optimum pH 7.0, after 120h of incubation with 40% inoculum volume and 50% moisture content. Enhanced production was obtained on addition of 1% W/V D-glucose, peptone, magnesium sulphate and 1% W/W glutamine as supplements which showed an increase to four folds. Using this optimized media components and parameters; the L-Glutaminase activity 496U/gds was obtained.

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Extracellular L-Glutaminase Production By Marine Brevundimonas Diminuta MTCC 8486

Cited by 5 publications

References 13 publications

Role of Nitrogen Source for L-Glutaminase Production from Fungal Strain using through Submerged Fermentation

Role of Nitrogen Source for L-Glutaminase Production from Fungal Strain using through Submerged Fermentation

Statistical monitoring of a web server for error rates: a bivariate time-series copula-based modeling approach

Optimization of Process Parameters for the Production of L-Glutaminase With Mixed Substrate by Solid State Fermentation Using Aspergillus Wentii MTCC 1901

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