Extracellular and Intracellular Dehydration Affect Urinary Bladder Reactivity to Vasopressin and Neurotransmitters of the Autonomic Nervous System in Anesthetized Female Wistar Rats.

Abstract

BackgroundThe effects of extracellular dehydration on the urinary bladder reactivity.AimTo investigate if different extracellular dehydration models affect urinary bladder reactivity to vasopressin and neurotransmitters of the autonomic nervous system in female Wistar rats.MethodsAdult female Wistar rats (N=6/group) one group the animals were in water deprivation for 24 hours and another group received a furosemide injection (50,0 mg/Kg S.C) and were maintained with sodium free diet. After all animals were anesthetized with 2% isoflurane in 100% O2 underwent to a cannulation of the femoral artery for mean arterial pressure and heart rate recordings. The urinary bladder was cannulated for intravesical pressure (IP) recordings. After baseline recording of the physiological parameters for 15 min, drugs (vasopressin 1 ng/mL, or acetylcholine 2 ug/mL, or noradrenaline 2 ug/mL) were administrated in situ (0.1 mL) on the urinary bladder and all the parameters were recorded for the control responses The AVP receptor subtypes were also labeled by immunofluorescence technique in the urinary bladder. Data are as mean±SE and were submitted to paired Student t‐test (p<0.001).ResultsThe water privation group responses in IP to vasopressin (39,5±1,0% vs. 110,1±1,9% control), acetylcholine (125,9±12,4% vs. 488,8±24,9% control), and noradrenaline (−17,5±1,0% vs. −67,6±1,6% control) were attenuated. Similarly the furosemide group the IP responses to vasopressin (42,8±2,8% vs. 110,1±1,9% control), acetylcholine (145,0±5,0% vs. 488,8±24,9% control), and noradrenaline (−28,8±1,0% vs. −67,6±1,6% control)were reduced. The immunofluorescence was showed a decrease in the amount of vasopressin receptors on the membrane of urinary bladder cells after dehydration in both models.ConclusionBoth models decreased the urinary bladder reactivity to vasopressin and to the neurotransmitters released by the autonomic nervous system innervation on the urinary bladder.Support or Funding InformationFinancial Support: FAPESP, CAPES and NEPAS.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.