Extended storage of AS‐1 and AS‐3 leukoreduced red blood cells for 15 days after deglycerolization and resuspension in AS‐3 using an automated closed system

Abstract

Leukoreduced AS-1 and AS-3 RBCs after frozen storage at -70 +/- 5 degrees C can be stored for up to 14 days when processing is performed with the ACP 215 system with resuspension of deglycerolized RBCs in AS-3.

Successful in vivo recovery and extended storage of additive solution (AS)‐5 red blood cells after deglycerolization and resuspension in AS‐3 for 15 days with an automated closed system

“…The ATP and 2,3-DPG levels after deglycerolization were reduced more quickly than what has been reported in liquid (never frozen) RBC units, as previously reported for AS-1-and AS-3-stored units. 5 All the study arms fulfilled the FDA criteria for 24-hour in vivo recovery (Fig. 1).…”

“…With the development of a functionally closed system for glycerolizing and deglycerolizing RBC units, 14-day extended storage of deglycerolized RBC units is currently available. [3][4][5] Deglycerolized RBCs after storage in citrate phosphate dextrose adenine (CPDA)-1, AS-1, and AS-3 have been cleared by the FDA for 14-day storage at 1 to 6°C when resuspended for storage in preservative solution (AS-3) with an automated closedsystem cell processor (ACP 215, Haemonetics Corp., Braintree, MA). 5 With this system, the risks of bacterial contamination during glycerolization and deglycerolization are minimized.…”

“…[3][4][5] Deglycerolized RBCs after storage in citrate phosphate dextrose adenine (CPDA)-1, AS-1, and AS-3 have been cleared by the FDA for 14-day storage at 1 to 6°C when resuspended for storage in preservative solution (AS-3) with an automated closedsystem cell processor (ACP 215, Haemonetics Corp., Braintree, MA). 5 With this system, the risks of bacterial contamination during glycerolization and deglycerolization are minimized. 2 This three-site randomized trial was designed to evaluate the in vivo and in vitro characteristics of leukoreduced (LR) and nonleukoreduced (NLR) AS-5 RBC units prepared from whole-blood (WB) donations, in systems designed for the collection of 500 mL.…”

Altered processing of thawed red cells to improve the in vitro quality during postthaw storage at 4°C

“…All fluids added are administered through 0.22‐µm bacteria barrier filters, and all tubing connections are achieved with a sterile connection device to maintain a closed system. When the ACP215 is used for processing, the FDA has approved extended postthaw storage for 14 days in the additive solution AS‐3 for RBCs that have been collected in CPDA‐1, CPD/AS‐1, or CP2D/AS‐3 1,2 . For RBCscollected in any other anticoagulant/additive solution (AS), the outdate is still 24 hours.…”

The American Rare Donor Program

“…The use of thawed RBCs is limited to 24-hour expiration when stored at 1°C to 6°C because of concerns related to potential bacterial contamination when a functionally open system is used to add and remove the glycerol. The recently developed ACP 215 system (Haemonetics, Braintree, Mass) has an extended expiration date of 14 days when stored at 1°C to 6°C for thawed, nonleukocyte-reduced, deglycerolized RBCs to 14 days [17].…”