1995
DOI: 10.1007/bf02634282
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Abstract: The potential use of porcine hepatocytes in a bioartificial liver device requires large quantities of viable and highly active cells. To facilitate the scaling up of the system, liver specific activities of hepatocytes should be maximized. One way of enhancing the specific activities is to cultivate hepatocytes as multicellular spheroids. Freshly isolated porcine hepatocytes form spheroids when cultivated in suspended cultures. These spheroids exhibit higher activities for a number of liver specific functions … Show more

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Cited by 92 publications
(51 citation statements)
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“…Optimization of bioreactor parameters and culturing conditions is expected to further increase the levels of liver-specific functions of hepatocyte spheroids. Phase I biotransformations via the cytochrome P450 family have yet to be characterized in a spheroid-entrapment BAL, though static monolayer cultures and spheroids entrapped into collagen gel have demonstrated ability to clear lidocaine to its metabolite monoethylglycinexylidide (MEGX) (Lazar et al, 1995a;Sielaff et al, 1995b). Initial characterizations of the BAL in vitro (Nyberg et al, 1992a) and in an anhepatic rabbit model (Nyberg et al, 1993) were conducted utilizing primary rat hepatocytes.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Optimization of bioreactor parameters and culturing conditions is expected to further increase the levels of liver-specific functions of hepatocyte spheroids. Phase I biotransformations via the cytochrome P450 family have yet to be characterized in a spheroid-entrapment BAL, though static monolayer cultures and spheroids entrapped into collagen gel have demonstrated ability to clear lidocaine to its metabolite monoethylglycinexylidide (MEGX) (Lazar et al, 1995a;Sielaff et al, 1995b). Initial characterizations of the BAL in vitro (Nyberg et al, 1992a) and in an anhepatic rabbit model (Nyberg et al, 1993) were conducted utilizing primary rat hepatocytes.…”
Section: Discussionmentioning
confidence: 99%
“…Albumin concentrations were determined by a sandwich enzyme-linked immunoassay (ELISA) as described elsewhere (Lazar et al, 1995a). Briefly, 96-well plates (Nunc, Naperville, IL) were precoated with 1:lOOO rabbit antiserum to porcine albumin (Cappel, Durham, NC) in pH 9.4 carbonate buffer.…”
Section: Albumin Concentration Measurementmentioning
confidence: 99%
“…In those studies employing mouse, rat or pig primary hepatocytes, cells were allowed to adhere to moderate to low attachment surface to enhance spheroid formation [33,35]. In other studies, primary hepatocytes were cultivated in suspension with a stirred bioreactor to form spheroids [36,37]. We recently reported three-dimensional aggregate differentiation of rat multipotent adult progenitor cells by allowing them to agglomerate in low attachment plates.…”
Section: Discussionmentioning
confidence: 99%
“…Again, such modalities are at an experimental stage, and their applicability remains to be confirmed. [67][68][69][70][71][72] …”
Section: Novel Culture Modalitiesmentioning
confidence: 99%