Extended Field-of-view and Increased-signal 3D Holographic Illumination with Time-division Multiplexing

Yang, Samuel J.; Allen, William E.; Kauvar, Isaac; Andalman, Aaron S.; Young, Noah P.; Kim, Christina K.; Marshel, James H.; Wetzstein, Gordon; Deisseroth, Karl

doi:10.1364/cosi.2016.cw5d.6

Cited by 6 publications

(10 citation statements)

References 15 publications

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“…The demonstrated MTF light shaping technique could therefore constitute the basis of a reliable and robust approach for 3D 'all-optical' brain circuits control on large scales, especially if combined with 3D imaging techniques 22,[63][64][65] . At the same time, its use is not only limited to neuronal activation, but could as 15 well be extended, in combination with camera detections 24,25 and multi-plane spatial demixing algorithms 66 , to fast volumetric functional imaging, using calcium or voltage sensors. More generally, any application relying on light patterning methods and non-linear phenomena, such as photopolymerization 67 , optical data storage and photolithography 68 , will benefit from this technique.…”

Section: Discussionmentioning

confidence: 99%

“…However, two-photon (2P) illumination is favourable to reach deeper regions in scattering tissues. 2P volumetric imaging was achieved either with scanning approaches, by quickly scanning single or multiple beams over planes or objects of interests [19][20][21][22][23] , or with parallel approaches, based on the simultaneous illumination of multiple targets in a volume and on extended depth of field detection 24,25 .…”

Section: Introduction 30mentioning

confidence: 99%

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Multiplexed temporally focused light shaping for high-resolution multi-cell targeting

Accanto

Tanese

Ronzitti

et al. 2017

Preprint

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15Patterning light at the single-cell level over multiple neurons in the brain is crucial for optogenetic photostimulation that can recapitulate natural activity patterns and, thereby, determine the role of specific components of brain activity in behavior. To this end we have developed a method for projecting three-dimensional, 2-photon excitation patterns that are confined to many individual neurons. The new versatile optical scheme generates multiple extended excitation spots in a large volume with micrometric 20 lateral and axial resolution. Two-dimensional temporally focused shapes are multiplexed several times over selected positions, thanks to the precise spatial phase modulation of the pulsed beam. This permits, under multiple configurations, the generation of tens of axially confined spots in an extended volume, spanning a range in depth of up to 500 µm. We demonstrate the potential of the approach by performing multi-cell volumetric excitation of photoactivatable GCaMP in the central nervous system of Drosophila 25 larvae, a challenging structure with densely arrayed and small diameter neurons, and by photoconverting the fluorescent protein Kaede in zebrafish larvae. Our technique paves the way for the optogenetic manipulation of a large number of neurons in intact circuits.

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Section: Discussionmentioning

confidence: 99%

Section: Introduction 30mentioning

confidence: 99%

Multiplexed temporally focused light shaping for high-resolution multi-cell targeting

Accanto

Tanese

Ronzitti

et al. 2017

Preprint

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“…Optimization of the fluorescence excitation based on pixelwise SNR may increase the SNR and reduce photodamage in functional voltage and calcium imaging using holographically sculpted light schemes Castanares et al, 2016;Dal Maschio et al, 2010;Foust et al, 2015;Nikolenko et al, 2008;Szabo et al, 2014;Tanese et al, 2017;Yang et al, 2015). SLS is characterized by high SNR and high acquisition rates.…”

Section: Discussionmentioning

confidence: 99%

High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning

et al. 2020

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Graphical AbstractHighlights d Smart line scanning (SLS) restricts imaging to the most informative image pixels d SLS substantially increases the signal-to-noise ratio of population GCaMP6 imaging d SLS leads to higher probability of detecting calcium events in population imaging d SLS achieves more precise identification of functional neuronal ensembles SUMMARY Two-photon functional imaging using genetically encoded calcium indicators (GECIs) is one prominent tool to map neural activity. Under optimized experimental conditions, GECIs detect single action potentials in individual cells with high accuracy. However, using current approaches, these optimized conditions are never met when imaging large ensembles of neurons. Here, we developed a method that substantially increases the signal-to-noise ratio (SNR) of population imaging of GECIs by using galvanometric mirrors and fast smart line scan (SLS) trajectories. We validated our approach in anesthetized and awake mice on deep and dense GCaMP6 staining in the mouse barrel cortex during spontaneous and sensory-evoked activity. Compared to raster population imaging, SLS led to increased SNR, higher probability of detecting calcium events, and more precise identification of functional neuronal ensembles. SLS provides a cheap and easily implementable tool for high-accuracy population imaging of neural GCaMP6 signals by using galvanometricbased two-photon microscopes.

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“…3) On the other hand, the chromatic aberration in the system also affects the effect of holographic projection. Color reproduction method based on time-division multiplexing is to load red, green and blue holograms in turns onto the same SLM [18,19]. This method requires the switching time of the light source and the hologram to be strictly consistent.…”

Section: Introductionmentioning

confidence: 99%

Holographic capture and projection system of real object based on tunable zoom lens

et al. 2020

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In this paper, we propose a holographic capture and projection system of real objects based on tunable zoom lenses. Different from the traditional holographic system, a liquid lens-based zoom camera and a digital conical lens are used as key parts to reach the functions of holographic capture and projection, respectively. The zoom camera is produced by combing liquid lenses and solid lenses, which has the advantages of fast response and light weight. By electrically controlling the curvature of the liquid-liquid surface, the focal length of the zoom camera can be changed easily. As another tunable zoom lens, the digital conical lens has a large focal depth and the optical property is perfectly used in the holographic system for adaptive projection, especially for multilayer imaging. By loading the phase of the conical lens on the spatial light modulator, the reconstructed image can be projected with large depths. With the proposed system, holographic zoom capture and color reproduction of real objects can be achieved based on a simple structure. Experimental results verify the feasibility of the proposed system. The proposed system is expected to be applied to microprojection and three-dimensional display technology.

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Extended Field-of-view and Increased-signal 3D Holographic Illumination with Time-division Multiplexing

Cited by 6 publications

References 15 publications

Multiplexed temporally focused light shaping for high-resolution multi-cell targeting

Multiplexed temporally focused light shaping for high-resolution multi-cell targeting

High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning

Holographic capture and projection system of real object based on tunable zoom lens

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