Expression profiling of circulating miRNAs in mouse serum in response to <i>Echinococcus multilocularis</i> infection

Guo, Xiaola; Zheng, Yadong

doi:10.1017/s0031182017000300

Cited by 45 publications

(47 citation statements)

References 44 publications

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“…The miRNA expression levels of mice were found to significantly in the sera and livers from mice in different stages of E. multilocularis infection [43,60] (Figure 2). Mmu-miR-146a-5p, mmu-miR-107-3p, mmu-miR-103-3p, and mmu-miR-21a-3p were found to be significantly upregulated after four weeks of infection.…”

Section: Mouse Mirnas Dysregulated During Infection With E Multilocumentioning

confidence: 99%

“…Large miRNAs, being identified in E. granulosus, E. multilocularis, and E. canadensis (G7), have been found to feature certain characteristics of genes in expression regulation, such as tissue and developmental stage specificity, in their respective hosts [18,19,43]. To date, the miRNA expression profiles of E. granulosus sensu stricto (76 known miRNAs, including adults, cysts, and protoscoleces) [44,45], E. canadensis (46 known miRNAs, including cysts and protoscoleces) [12,46], and E. multilocularis (46 known miRNAs, including cysts) [1] have been reported (Table 1).…”

Section: Widespread Expression Of Mirnas In Echinococcus Spp Accordimentioning

confidence: 99%

“…The conservation of miRNAs that are involved in Echinococcus regulation reflects the complex and sophisticated adaptations, which are necessary for different environments, present within the life cycles of parasitic species. High-throughput sequencing and miRNA microarray analyses identified dysregulated miRNAs present during parasite infection in natural hosts and animal models that were present in relevant cells, tissues, and blood; demonstrating the importance of these miRNAs in host responses to pathogen challenges [19,43,55]. Recent research results have shown that circulating non-coding RNAs, including miRNAs and lncRNAs, can be stably detected in the blood of hosts that were infected with E. granulosus and E. multilocularis [19,43,56] (Table 3).…”

Section: Common Mirnas In Echinococcus Sppmentioning

confidence: 99%

“…High-throughput sequencing and miRNA microarray analyses identified dysregulated miRNAs present during parasite infection in natural hosts and animal models that were present in relevant cells, tissues, and blood; demonstrating the importance of these miRNAs in host responses to pathogen challenges [19,43,55]. Recent research results have shown that circulating non-coding RNAs, including miRNAs and lncRNAs, can be stably detected in the blood of hosts that were infected with E. granulosus and E. multilocularis [19,43,56] (Table 3). These stably circulating non-coding RNAs have the potential to provide us with an understanding of their roles in the host-parasite interaction, development, and growth; and, could potentially serve as diagnostic targets and therapeutic candidates.…”

Section: Common Mirnas In Echinococcus Sppmentioning

confidence: 99%

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miRNAs and lncRNAs in Echinococcus and Echinococcosis

Yan

Yuán

et al. 2020

IJMS

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Echinococcosis are considered to be potentially lethal zoonotic diseases that cause serious damage to hosts. The metacestode of Echinococcus multilocularis and E. granulosus can result in causing the alveolar and cystic echinococcoses, respectively. Recent studies have shown that non-coding RNAs are widely expressed in Echinococcus spp. and hosts. In this review, the two main types of non-coding RNAs—long non-coding RNAs (lncRNAs) and microRNAs (miRNAs)—and the wide-scale involvement of these molecules in these parasites and their hosts were discussed. The expression pattern of miRNAs in Echinococcus spp. is species- and developmental stage-specific. Furthermore, common miRNAs were detected in three Echinococcus spp. and their intermediate hosts. Here, we primarily focus on recent insights from transcriptome studies, the expression patterns of miRNAs and lncRNAs, and miRNA-related databases and techniques that are used to investigate miRNAs in Echinococcus and echinococcosis. This review provides new avenues for screening therapeutic and diagnostic markers.

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Section: Mouse Mirnas Dysregulated During Infection With E Multilocumentioning

confidence: 99%

Section: Widespread Expression Of Mirnas In Echinococcus Spp Accordimentioning

confidence: 99%

Section: Common Mirnas In Echinococcus Sppmentioning

confidence: 99%

Section: Common Mirnas In Echinococcus Sppmentioning

confidence: 99%

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miRNAs and lncRNAs in Echinococcus and Echinococcosis

Yan

Yuán

et al. 2020

IJMS

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“…In the case of Schistosoma japonicum , high serum level of hepatic miR-223 was correlated with active infection, and responsiveness to praziquantel therapy was characterized by a return to normal levels [38]. E. multilocularis was also found to quantitatively modulate circulating and liver miRNAs in a mouse model of secondary (intraperitoneal) infection [39, 40]. Similarly, the closely related E. granulosus also caused changes in the intestinal miRNA transcriptome of Kazakh sheep [41].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Hepatic MicroRNAs in Response to Early Stage Echinococcus multilocularis Egg Infection in C57BL/6 mice

Boubaker

Strempel

Hemphill

et al. 2019

Preprint

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22In this study, we present a comprehensive analysis of the hepatic miRNA transcriptome in 23 mice suffering from experimental primary alveolar echinococcosis (AE), a parasitic infection 24 caused upon ingestion of Echinococcus multilocularis (E. multilocularis) eggs. At one month 25 post-infection, infected C57BL/6 mice, along with non-infected control mice, were 26 euthanized. Subsequently livers were collected and used for small RNA library preparation 27 and next-generation sequencing (NGS). The most significantly dysregulated hepatic miRNAs 28 were validated by Stem-loop RT-qPCR. We identified 28 miRNAs with significantly altered 29 expression levels upon infection with E. multilocularis. Of these, 9 were up-regulated (fold 30 change (FC) ≥ 1.5) and 19 were down-regulated (FC ≤ 0.66) as compared to the non-infected 31 controls. In infected liver tissues, mmu-miR-148a-3p and mmu-miR-101b-3p were 8-and 6-32 fold down-regulated, respectively, and the expression of mmu-miR-22-3p was reduced by 33 50%, compared to non-infected liver tissue. Conversely, significantly higher hepatic levels 34 were noted for Mus musculus (mmu)-miR-21a-5p (FC = 2.3) and mmu-miR-122-5p (FC = 35 1.8). Down-regulated miRNAs were highly enriched in Reactome and KEGG pathways of 36 angiogenesis and fatty acids biosynthesis. Moreover, relative mRNA expression levels of 37 three pro-angiogenic (VEGFA, MTOR and HIF1-α) and two lipogenic (FASN and ACSL1) 38 genes were significantly higher in livers of E. multilocularis infected mice. 39Lastly, we studied the issue related to functionally mature arm selection preference (5p and/or 40 3p) from the miRNA precursor and showed that 9 pre-miRNAs exhibited different arm 41 selection preferences in normal versus infected liver tissues. Our study provides first evidence 42 of miRNA involvement in liver pathogenesis during AE. Our future research will focus on the 43 characterization of miRNA transcriptome patterns in more advanced AE-stages towards the 44 assessment of microRNA therapy for AE, and experimentally address functional 45 characteristics of selected features presently found. 48of microRNAs (miRNAs), a class of small non-coding RNAs involved in negative regulation 49 of gene expression. Herein, we revealed that significant alterations of miRNA expression 50 occurred in murine liver subsequently to experimental infection with Echinococcus 51 multilocularis (E. multilocularis) eggs when compared to non-infected controls. 52At the early stage of murine AE, hepatic miRNAs were mainly downregulated. Respective 53 target genes of the most extensively downregulated miRNAs were involved in angiogenesis 54 and fatty acid synthesis. Indeed, angiogenic and lipogenic genes were found to be 55 significantly higher expressed in E. multilocularis infected livers relative to non-infected 56 controls. These boosted cellular pathways are advantageous for development of the E. 57 multilocularis metacestodes, since this larval stage is not able to undertake de novo fatty acid 58 synthesis, and angiogenesis ...

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The regulatory role of differential microRNA expressions on cellular inflammatory factors IL‐6 and IL‐10 in Echinococcus granulosus‐induced anaphylaxis

Wang,

Yu,

Kulaixi

et al. 2023

Immunity Inflam & Disease

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ObjectiveTo determine the pathogenesis and molecular targets of anaphylaxis caused by hydatid cyst fluid leakage.MethodsFirst, Balb/c mice were infected with Echinococcus granulosus, and then the anaphylaxis model was developed. The mice were separated into: anaphylaxis caused by the cystic echinococcosis group (ANPC), the cystic echinococcosis without anaphylaxis group (CE group), and the normal control group (CTRL). Following this, the spleen tissue was collected for microRNA (miRNA) sequencing. Using bioinformatics analysis, differentially expressed miRNAs (DEMs) were identified. Then, through the use of protein–protein interaction (PPI) networks, the key target genes for miRNA regulation associated with echinococcosis‐induced anaphylaxis were identified.ResultsANPC and CE groups have 29 and 39 DEMs compared to the CTRL group, respectively. Based on these 25 DEMs, interactions between miRNA and mRNA were screened, and 174 potential target genes were identified. We performed gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis on these 174 target genes, and the results revealed that the three pathways with the highest enrichment were the PI3K‐Akt signaling pathway, FoxO signaling pathway, and Focal adhesion. The interaction analysis of PPI and miRNA‐hub gene networks revealed that interleukin 6 (IL‐6) was regulated by miR‐146a‐5p and miR‐149‐5p, while IL‐10 was regulated by miR‐29b‐3p and miR‐29c‐3. Using reverse transcription polymerase chain reaction, we found that the miRNAs regulating IL‐6 and IL‐10 were significantly upregulated in the ANPC group, and there are three pathways involved in that process: Pathways of PI3K‐Akt signaling, FoxO signaling, and Focal adhesion. IL‐6 and IL‐10 play an important role in cellular pyroptosis and apoptosis. Therefore, the aforementioned results provide significant reference value for elucidating the mechanism of cellular pyroptosis and apoptosis in echinococcosis‐induced anaphylaxis, and for formulating tissue and organ protection strategies for patients with cystic echinococcosis when anaphylaxis is triggered by hydatid cyst rupture.

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Expression profiling of circulating miRNAs in mouse serum in response to Echinococcus multilocularis infection

Cited by 45 publications

References 44 publications

miRNAs and lncRNAs in Echinococcus and Echinococcosis

miRNAs and lncRNAs in Echinococcus and Echinococcosis

Regulation of Hepatic MicroRNAs in Response to Early Stage Echinococcus multilocularis Egg Infection in C57BL/6 mice

The regulatory role of differential microRNA expressions on cellular inflammatory factors IL‐6 and IL‐10 in Echinococcus granulosus‐induced anaphylaxis

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