Expression profiles of defense genes in cassava storage roots upon exposure to Phytopythium sp., causal agent of soft root rot disease

Lima, Aline Medeiros; Moura, Elisa Ferreira; Ishida, A. K. N.; Pereira, Angelo Cleiton da Costa; Reis, Sávio Pinho dos; Souza, Cláudia Regina Batista de

doi:10.1016/j.pmpp.2018.09.001

Cited by 7 publications

(3 citation statements)

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“…Some previous reports have shown that resistance to cassava root rot has a polygenic character [ 23 , 55 ]. Quantitative resistance is controlled by multiple minor genes on the plant, thereby promoting partial resistance to the pathogen and a decrease in the severity of symptoms and/or the progression of epidemics over time [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Overall understanding of the genetic mechanisms responsible for the various levels of resistance to root rot disease in field conditions among cassava genotypes remains underexplored. The studies developed to date have focused on searching for sources of resistance [ 10 , 20 ] and identifying the possible genes involved in defense mechanisms [ 23 , 55 ] using the in vitro methodology of root inoculation. However, in order to characterize the disease and the behavior of cassava genotypes in the field, it is necessary to consider the different defense mechanisms not only of the root system tissues, but also the shoot yields of the genotypes used in planting [ 29 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, in order to characterize the disease and the behavior of cassava genotypes in the field, it is necessary to consider the different defense mechanisms not only of the root system tissues, but also the shoot yields of the genotypes used in planting [ 29 , 46 ]. Although the genes associated with plant response to pathogens and oxidative processes have been described as possibly involved in root resistance [ 23 , 55 ], it is essential to identify and quantify which genes have constitutive expression, as well as which are subject to the control of temporal-specific and tissue-specific expression in cassava versus root rot interaction.…”

Section: Discussionmentioning

confidence: 99%

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Genome-wide association study and selection for field resistance to cassava root rot disease and productive traits

et al. 2022

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Cassava root rot disease is caused by a complex of soil-borne pathogens and has high economic impacts because it directly affects the tuberous roots, which are the main commercial product. This study aimed to evaluate cassava genotypes for resistance to root rot disease in a field with a previous history of high disease incidence. It also aimed to identify possible genomic regions associated with field resistance based on genome-wide association studies. A total of 148 genotypes from Embrapa Mandioca and Fruticultura were evaluated over two years, including improved materials and curated germplasms. Analysis of phenotypic data was conducted, as well as a genomic association analysis, based on the general linear model, mixed linear model, and fixed and random model circulating probability unification. The observed high disease index (ω) was directly correlated with genotype survival, affecting plant height, shoot yield, and fresh root yield. The genotypes were grouped into five clusters, which were classified according to level of root rot resistance (i.e., extremely susceptible, susceptible, moderately susceptible, moderately resistant, and resistant). The 10 genotypes with the best performance in the field were selected as potential progenitors for the development of segregating progenies. Estimates of genomic kinship between these genotypes ranged from -0.183 to 0.671. The genotypes BGM-1171 and BGM-1190 showed the lowest degree of kinship with the other selected sources of resistance. The genotypes BGM-0209, BGM-0398, and BGM-0659 showed negative kinship values with most elite varieties, while BGM-0659 presented negative kinship with all landraces. A genome-wide association analysis detected five significant single nucleotide polymorphisms related to defense mechanisms against biotic and abiotic stresses, with putative association with fresh root yield in soil infested with root rot pathogens. These findings can be utilized to develop molecular selection for root rot resistance in cassava.

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Section: Discussionmentioning

confidence: 99%