Expression pattern, genomic structure, and promoter analysis of the gene encoding stilbene synthase from Chinese wild Vitis pseudoreticulata

Abstract

The gene encoding stilbene synthase (STS) plays a central role in many biochemical and physiological actions, and its metabolite resveratrol possesses broad-spectrum resistance to pathogens, as well as diverse pharmacological properties, notably an anticancer effect. Here, we report the expression analysis of the gene encoding STS and its promoter function from a powdery mildew (PM)-resistant Chinese wild Vitis pseudoreticulata, and compare it with two PM-susceptible cultivated grapevines, Vitis vinifera cvs. Carignane and Thompson Seedless. We show an unusual expression pattern of STS in V. pseudoreticulata, which differs markedly from that of the cultivated species. Sequence comparisons reveal that the genomic DNA sequences encoding STS in the three grapevines are highly conserved, but a novel residue mutation within the key motif of STS is solely present in V. pseudoreticulata. Moreover, the STS promoter in V. pseudoreticulata displays a significantly different structure from that found in the two V. vinifera. The three promoter-driven GUS differential expression patterns in transformed tobacco plants induced with Alternaria alternata, methyl jasmonate, and wounding indicated that the structurally different STS promoter of V. pseudoreticulata is responsible for its specific regulatory function. We also demonstrate that the expression of STS genes from their native promoters are functional in transformed tobacco and retain pathogen inducibility. Importantly, the genomic DNA-2 of V. pseudoreticulata under its native promoter shows good induction and the maximum level of resveratrol content. These findings further our understanding of the regulation of STS expression in a resistant grapevine and provide a new pathogen-inducible promoter system for the genetic improvement of plant disease resistance.

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

“…If A. ipaënsis has behavior similar to A. hypogaea, the best point for analysis of resveratrol in this species can be reached 15 h after treatment. This was also observed in species of grapevine (wild and cultivated) one sequence of RS had different expression levels in various hours post inoculation with Uncinula necator for each genotype, showing a speciesspecific expression profile (Xu et al, 2011). This is the study trial of RS using RT-qPCR in Arachis and the first one in wild Arachis species.…”

Molecular Characteristics and Biochemical Functions of VpPR10s from Vitis pseudoreticulata Associated with Biotic and Abiotic Stresses

“…For example, the Chinese wild grapevine Vitis pseudoreticulata accession “Baihe-35-1” has relatively highly resistance to a number of fungi, and especially to E. necator [6,7]. These wild grapevine germplasm resources offer the opportunity to mine novel disease-resistance genes and so accelerate the genetic improvement of our existing V. vinifera germplasm resources.…”

The Phytoalexin Resveratrol Regulates the Initiation of Hypersensitive Cell Death in Vitis Cell

“…Engineering the StSy into plants of interest results in resveratrol accumulation and elevates pathogen resistance in some crops such as rice [7], tomato [8], or barley [9]. Stilbene synthases are typically organised in gene families with high sequence homology of individual members, but different regulatory features in their promotors [10]. For the sake of simplicity, in this study, the term stilbene synthase is used to designate this family of enzymes.…”