Expression pattern and subcellular localization of Arabidopsis purple acid phosphatase AtPAP9

Zamani, Katayoun; Lohrasebi, Tahmineh; Sabet, Mohammad; Malboobi, Mohammad Ali; Mousavi, Amir

doi:10.1016/j.gep.2013.08.001

Cited by 15 publications

(13 citation statements)

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“…There are 29 PAPs in Arabidopsis, with different spatial and temporal expression patterns (Li et al 2002;Zhu et al 2005). AtPAP10, AtPAP12 and AtPAP26 are three major Pi starvation-responsive secretory and intracellular APases (Robinson et al 2012a, b;Wang et al 2011Wang et al , 2014; AtPAP15 is likely to be involved in mobilizing Pi reserves during seed and pollen germination (Kuang et al 2009); AtPAP9 and AtPAP5 are probably involved in defense against plant pathogens (Ravichandran et al 2013;Zamani et al 2014); and AtPAP2, which is targeted to both plastids and mitochondria, is involved in plant growth . These data collectively suggest that PAPs play important roles in a wide range of physiological functions.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a tomato purple acid phosphatase during seed germination and seedling growth under phosphate stress

2015

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SlPAP1 is a phosphate starvation responsive purple acid phosphatase during tomato seed germination. Future research on its family members in tomato might improve the phosphate stress tolerance. Phosphate deficiency is a major constraint upon crop growth and yield. In response to phosphate deficiency, plants secrete acid phosphatases (APases) to scavenge organic phosphate from soil. In this study, we investigated the impact of Pi starvation on germination and seedling growth of tomato, and we then cloned and characterized a phosphate starvation responsive purple APase (SlPAP1) that expressed during tomato seedling growth. Our results showed that phosphate deficiency reduced germination and growth rates of tomato, and also increased intracellular and secretory APase activity in a concentration-dependent manner. An in-gel activity assay found that two APases of 50 and 75 kDa were secreted during conditions of phosphate deficiency. SlPAP1 is a single copy gene belonging to a small gene family. It was expressed as a cDNA of approximately 1.5 kbp encoding a secreted glycoprotein of 470 amino acids. Northern blot analysis showed that SlPAP1 was specifically expressed in root tissue during phosphate deficiency. SlPAP1 had high sequence identity (56-89%) with other plant PAPs and contained highly conserved metal-binding residues. SlPAP1 is the first PAP to be cloned and characterized from tomato. This study provides useful information for future research on PAP family members in tomato, leading to better understanding of phosphate deficiency in this important crop plant.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of a tomato purple acid phosphatase during seed germination and seedling growth under phosphate stress

2015

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“…Genome annotation identified 29 putative PAP genes in Arabidopsis thaliana, several of which are induced during Pi deprivation (Tran et al, 2010a). The variation and complexity of AtPAP1-29 expression has been well documented (Zhu et al, 2005;Veljanovski et al, 2006;Zhang et al, 2008;Tran et al, 2010a,b;Wang et al, 2011Wang et al, , 2014Robinson et al, 2012a;Zamani et al, 2014). To assess the function of specific AtPAP genes in the metabolic adaptations of Pi-starved (ÀPi) Arabidopsis, we have purified, characterized and identified native intracellular and secreted AtPAP isozymes that are up-regulated during Pi stress.…”

Section: Introductionmentioning

confidence: 99%

The cell wall‐targeted purple acid phosphatase AtPAP25 is critical for acclimation of Arabidopsis thaliana to nutritional phosphorus deprivation

et al. 2014

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SUMMARYPlant purple acid phosphatases (PAPs) belong to a relatively large gene family whose individual functions are poorly understood. Three PAP isozymes that are up-regulated in the cell walls of phosphate (Pi)-starved (ÀPi) Arabidopsis thaliana suspension cells were purified and identified by MS as AtPAP12 (At2g27190), AtPAP25 (At4g36350) and AtPAP26 (At5g34850). AtPAP12 and AtPAP26 were previously isolated from the culture medium of ÀPi cell cultures, and shown to be secreted by roots of Arabidopsis seedlings to facilitate Pi scavenging from soil-localized organophosphates. AtPAP25 exists as a 55 kDa monomer containing complex NX(S/T) glycosylation motifs at Asn172, Asn367 and Asn424. Transcript profiling and immunoblotting with anti-AtPAP25 immune serum indicated that AtPAP25 is exclusively synthesized under ÀPi conditions. Coupled with potent mixed-type inhibition of AtPAP25 by Pi (I 50 = 50 lM), this indicates a tight feedback control by Pi that prevents AtPAP25 from being synthesized or functioning as a phosphatase except when Pi levels are quite low. Promoter-GUS reporter assays revealed AtPAP25 expression in shoot vascular tissue of ÀPi plants. Development of an atpap25 T-DNA insertion mutant was arrested during cultivation on soil lacking soluble Pi, but rescued upon Pi fertilization or complementation with AtPAP25. Transcript profiling by quantitative RT-PCR indicated that Pi starvation signaling was attenuated in the atpap25 mutant. AtPAP25 exhibited near-optimal phosphatase activity with several phosphoproteins and phosphoamino acids as substrates. We hypothesize that AtPAP25 plays a key signaling role during Pi deprivation by functioning as a phosphoprotein phosphatase rather than as a non-specific scavenger of Pi from extracellular P-monoesters.

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“…Some APase genes, such as the AtPAP10 gene, encode a root surface-bound enzyme which has a critical role in plant tolerance and adaptation to Pi deficiency [22]. In addition, another study about the effects of overexpressing the AtPAP18 gene in Arabidopsis on the P status, revealed the great potential of this gene in reducing the consumption of chemical Pi fertilizers, since the plants overexpressing the gene could uptake more Pi from the soil and had the ability to remobilize the Pi from the internal resources [12]. Some APase genes have been cloned and overexpressed in model plant species [10,13,23,24] but only a few of these genes have been described in soybean and other important crops [6,9,11,16].…”

Section: Hairy Root Induction and Gfp Assaymentioning

confidence: 99%

“…Also, transgenic plants exhibited enhanced P efficiency and better root and shoot growth rates versus the controls [6]. Overexpression of AtPAP18 in Nicotiana tabbacum led to significantly increased acid phosphatase activity, total P and Pi contents, resulting in improved biomass production in both Pi-deficient and Pi-sufficient conditions [12,13].…”

Section: Introductionmentioning

confidence: 97%

Agrobacterium rhizogenes transformed soybeans with AtPAP18 gene show enhanced phosphorus uptake and biomass production

Younessi-Hamzekhanlu

Izadi-Darbandi

Malboobi

et al. 2018

Biotechnology & Biotechnological Equipment

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Low-phosphorus stress is a challenging factor in limiting plant development. Soybean is cultivated in soils often low in phosphorus. However, on average 65% of total P is in the form of organic phosphates, which are unavailable to plants unless hydrolyzed to release inorganic phosphate. One approach for enhancing crop P acquisition from organic P sources is boosting the activity of acid phosphatases (APases). This study seeks to understand the role of an Arabidopsis (Arabidopsis thaliana) purple APase gene (AtPAP18) in soybean. Thus, the gene was isolated and a final vector (AtPAP18/pK7GWG2D) was built. Composite soybean plants were created using Agrobacterium rhizogenes-mediated transformation. A. rhizogenes K599 carrying the AtPAP18/pK7GWG2D vector with egfp as a reporter gene was used for soybean hairy root transformation. Analysis of Egfp expression detected fluorescence signals in transgenic roots, whereas there was no detectable fluorescence in control hairy roots. The enzyme assay showed that the APase activity increased by 2-fold in transgenic hairy roots. The transformed hairy roots displayed an increase in plant soluble P and total P contents, as compared with the control plants, leading to improved biomass production. RT-PCR analysis revealed high expression levels of AtPAP18 in transformed hairy roots. It is noteworthy that these primers amplified no PAP18 transcript in control hairy roots. Taken together, the findings demonstrated that overexpression of the AtPAP18 gene offers an operative tactic to reduce the utilization of inorganic phosphorus (Pi) fertilizer through increased acquisition of soil Pi, especially improving the crop yield on soils low in available P.

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Expression pattern and subcellular localization of Arabidopsis purple acid phosphatase AtPAP9

Cited by 15 publications

References 50 publications

Molecular characterization of a tomato purple acid phosphatase during seed germination and seedling growth under phosphate stress

Molecular characterization of a tomato purple acid phosphatase during seed germination and seedling growth under phosphate stress

The cell wall‐targeted purple acid phosphatase AtPAP25 is critical for acclimation of Arabidopsis thaliana to nutritional phosphorus deprivation

Agrobacterium rhizogenes transformed soybeans with AtPAP18 gene show enhanced phosphorus uptake and biomass production

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