Expression of WOX and PIN genes during somatic and zygotic embryogenesis in Medicago truncatula

Tvorogova, Varvara E.; Lebedeva, Maria A.; Лутова, Л. А.

doi:10.1134/s1022795415120121

Cited by 34 publications

(23 citation statements)

References 24 publications

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“…Along with the WOX TF the PINFORMED (PIN) genes that codified PIN1, PIN4 and PIN7 proteins are involved in the formation of the apical-basal axis of the embryo providing the intercellular transport of the auxin (Tvorogova et al 2015). PIN1 and PIN7 proteins act directly in the apicalbasal auxin transport in early embryogenesis.…”

Section: Molecular View Of Abnormalities In Plant Embryogenesismentioning

confidence: 99%

“…Two important PIN proteins are expressed in the embryo patterning (PIN1 and PIN7). Mutation or inactivation of genes that codify these proteins affect the establishment of apicalbasal polarity in the early embryo formation (Tvorogova et al 2015). Another important protein that is involved in the cell fate and embryo patterning is the WRKY protein.…”

Section: Alanyl-trnasynthetasementioning

confidence: 99%

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Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

105

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Somatic embryogenesis is a morphogenetic event where somatic cells have the ability to produce embryos without gamete fusion. It is used as a technique for plant mass propagation. It is a process that has six well defined steps such as induction, expression, development, maturation, germination and plant conversion. These steps are characterized by distinct physiological, morphological and molecular events. Although somatic embryogenesis has been established in several plant species, there remains many problems to be solved. The main problem in somatic embryogenesis is the large number of abnormal embryos produced which cannot germinate nor convert into normal plants. Abnormalities in somatic embryos (SE) can be generated by genetic or epigenetic changes in the DNA. These changes in the DNA can be influenced by external factors such as the use of plant growth regulators and mutagenic substances or stress factors applied to the plant tissue such as high and low temperatures, drought, salinity, and heavy metals. Abnormalities generated by genetic changes in the DNA are hardly reversible; however, abnormalities generated by epigenetic changes may be reversible and the abnormal embryos are able to produce normal plants in most cases. This review focuses on the identification of the main factors that can cause abnormal SE development in different plant species, suggest how SE abnormalities are related to somaclonal variations and identify which genes may be involved with embryo abnormalities. Zygotic embryo abnormalities in Arabidopsis thaliana mutants are listed with the aim to understand the main genetic mechanisms involved in embryo aberrations. Key messageThe abnormalities in somatic embryos are related to the use of 2,4-D in most of the published protocols, this sintetic auxin disrupts the endogenous auxin balance and the auxin polar transportation interfering with the embryo apical-basal polarity.

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Section: Molecular View Of Abnormalities In Plant Embryogenesismentioning

confidence: 99%

Section: Alanyl-trnasynthetasementioning

confidence: 99%

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

105

View full text Add to dashboard Cite

show abstract

“…To study the molecular regulation mechanism of plant SE, the transcriptomes analysis were carried out to identify SE related genes by RNA-seq in plant species, including herbaceous plants such as Arabidopsis [16], Gossypium hirsutum [17], Maize [18], Strawberry [19], Rice [20], etc., and woody plants such as norway spruce [21], coconut plam [22], brazilian pine [23], camphor tree [24], papaya [25], Dimocarpus longan [26] and so on. These study demonstrated regulation mechanisms of SE at molecular level, and several potential somatic embryogenesis key genes were identi ed, such as late embryogenesis abundant (LEA) protein [25], somatic embryogenesis receptor-like kinase (SERK) [27,28], Leafy Cotyledon [28,29], AGAMOUS-like 15 [30,31], BABYBOOM [28,32], WUSCHEL [33,34], and WUSCHEL homeobox 2 [28,35].…”

Section: Introductionmentioning

confidence: 93%

Transcriptomes Analysis Reveals Novel Insight Into the Molecular Mechanisms of Somatic Embryogenesis in Hevea Brasiliensis

Wang

Zhou

et al. 2020

Preprint

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Background Somatic embryogenesis (SE) is a promising technology for plant vegetative propagation, which has an important role in tree breeding. Though rubber tree (Hevea brasiliensis Muell. Arg.) SE has been founded, few late SE-related genes had been identified and the molecular regulation mechanisms of late SE still not well understood. Results In this study, the transcriptomes of embryogenic calluses (EC), primary embryo (PE), cotyledonary embryo (CE), abnormal embryo (AE), mature cotyledonary embryo (MCE) and withered abnormal embryo (WAE) were analyzed. A total of 887,852,416 clean reads were generated, more than 85.92% of the clean reads were mapped to the rubber tree genome. The de novo assembly generated 36937 unigenes. The differentially expressed genes (DEGs) were identified in the pairwise comparisons of CE vs. AE and MCE vs. WAE, respectively. The specific common DEGs mainly involved in phytohormones signaling pathway, biosynthesis of phenylpropanoid and starch and sucrose metabolism. Among them, hormone signal transduction related genes were significantly enriched, especially the auxin signaling factors (AUX-like1, GH3.1, SAUR32-like, IAA9-like, IAA14-like, IAA27-like, IAA28-like and ARF5-like). The transcript factors including WRKY40, WRKY70, MYBS3-like, MYB1R1-like, AIL6 and bHLH93-like were characterized as molecular markers for rubber tree late SE. CML13, CML36, CAM-7, SERK1 and LEAD-29-like were related to rubber tree late SE. In addition, histone modification had crucial roles during rubber tree late SE. Conclusions This study provides important information to elucidate the molecular regulation during rubber tree late SE.

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“…In our previous studies, three new M. truncatula genes of the WOX family, that are expressed during SE, were found: MtWOX9-1, MtWOX11-like and STENOFOLIA (MtWOX1) (Tvorogova et al, 2015). It was further shown that overexpression of STF or MtWOX9-1 stimulates the emergence of somatic embryos (Tvorogova et al, 2016(Tvorogova et al, , 2019.…”

Section: Introductionmentioning

confidence: 95%

Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression

et al. 2019

Self Cite

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Somatic embryogenesis (SE) is the development of embryo-like structures from somatic plant tissues. This process rarely can be observed in nature, but for many plant species, in vitro protocols are developed, which allow to obtain somatic embryos formation directly from tissues of plant explant or from the embryogenic callus. SE is widely used for plant propagation and transformation; therefore, the search for SE stimulators and revealing of the mechanisms of their functioning are very important for biotechnology. Among the SE regulators, proteins of the WOX family play significant roles. WOX (WUSCHEL-RELATED HOMEOBOX) is a homeodomain-containing transcription factor family. Different WOX genes function in different plant organs and tissues, maintaining meristem activity and regulating cell proliferation and differentiation. Recently, we have shown that transcription factor MtWOX9-1, belonging to the WOX family, can stimulate SE in the Medicago truncatula callus culture. In this research, transcriptomic analysis of highly embryogenic calli with MtWOX9-1 overexpression was performed in comparison to wildtype calli. It was shown that MtWOX9-1 overexpression led to the activation of several groups of genes, including genes related to cell division, tissue diff erentiation, and seed development. Enriched GO pathways included several groups related to histone methyltransferase activity as well as DNA methylation and chromatin binding, suggesting major epigenetic changes that occur in call overexpressing MtWOX9-1. Using Medicago Truncatula Gene Expression Atlas, we also iden tified a group of genes coding for transcription factors that were both coexpressed with MtWOX9-1 in different plant organs and differentially expressed in our samples. These genes are putative targets of MtWOX9-1, and they may act in the same pathway with this regulator during SE.

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Expression of WOX and PIN genes during somatic and zygotic embryogenesis in Medicago truncatula

Cited by 34 publications

References 24 publications

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Transcriptomes Analysis Reveals Novel Insight Into the Molecular Mechanisms of Somatic Embryogenesis in Hevea Brasiliensis

Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression

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