Expression of vesicular glutamate transporters 1 and 2 in the cells of origin of the rat thalamostriatal pathway

Barroso‐Chinea, Pedro; Castle, M.; Aymerich, Marta; Lanciego, José L.

doi:10.1016/j.jchemneu.2007.08.001

Cited by 47 publications

(34 citation statements)

References 25 publications

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“…In the thalamus, vGluT1 stains the mediodorsal, laterodorsal, ventromedial nuclei, lateral and medial geniculate nuclei, whereas vGluT2 labels the lateral and medial habenular nuclei, anterior nucleus, lateral and medial geniculate nuclei, ventrolateral, paraventricular, parafascicular nuclei. vGluT1 neurons in the cortex project to the striatum and thalamus, while vGluT2 neurons in the thalamus send efferents to the cortex and striatum (Fujiyama et al, 2001;Varoqui et al, 2002;Raju et al, 2006;Barroso-Chinea et al, 2008). In the hypothalamus, vGluT2 immunoreactivity is much stronger and larger than vGluT1.…”

Section: Distribution Of Vglut2mentioning

confidence: 99%

Immunohistochemical localization of vesicular glutamate transporter 2 (vGluT2) in the central nervous system of the pigeon (Columba livia)

Atoji

2011

J of Comparative Neurology

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Glutamatergic neurons are distributed widely in the telencephalic pallium of birds, but their targets are unknown. In the present study, a polyclonal antibody was produced against pigeon vesicular glutamate transporter 2 (vGluT2) and was used for Western blot and immunohistochemistry to detect projection targets of glutamatergic neurons in the pigeon central nervous system. The molecular weight of vGluT2 was measured at 65 kDa. vGluT2 immunoreactivity was observed in neuropil, but not in neuronal cell bodies or glia. Immunoreactive neuropil generally appeared homogeneous, but fine granules or puncta were found in many areas or nuclei. The telencephalon showed strong immunoreactivity, except for the globus pallidus. In particular, the mesopallium and hippocampal formation revealed the most intense immunostaining. In the diencephalon, vGluT2 immunoreactivity was intense in the dorsal thalamus and hypothalamus. In the midbrain, strong immunostaining was seen in the periaqueductal gray, the dorsal part of the lateral mesencephalic nucleus, and the isthmo-optic nucleus. The optic tectum showed moderate immunoreactivity. In the cerebellar cortex, glomeruli in the granular layer were intensely immunoreactive, and the molecular layer showed intensely homogeneous immunostaining. In the caudal brainstem, the cochlear magnocellular and angular nuclei showed strongly immunoreactive puncta around neuronal cell bodies. In the spinal cord, the dorsal horn revealed moderate immunoreactivity and the marginal nucleus was strongly immunoreactive. Ultrastructural observations revealed that vGluT2 immunoreactivity is localized in asymmetric, presynaptic terminals. The present results indicate an extensive distribution of glutamatergic projections and circuits in the avian central nervous system.

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Section: Distribution Of Vglut2mentioning

confidence: 99%

Immunohistochemical localization of vesicular glutamate transporter 2 (vGluT2) in the central nervous system of the pigeon (Columba livia)

Atoji

2011

J of Comparative Neurology

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“…The development of transgenic mice that express enhanced green fluorescent protein (EGFP) under the D 1 and D 2 dopamine receptor promoters (Gong et al, 2003;Valjent et al, 2009) and thus identify direct and indirect pathway MSNs, respectively, together with the fact that subtypes of vesicular glutamate transporters are selective molecular markers of cortical and intralaminar thalamic afferents (VGluT1 and VGluT2, respectively) (Fremeau et al, 2001(Fremeau et al, , 2004Herzog et al, 2001;Kaneko and Fujiyama, 2002;Fujiyama et al, 2004;Raju et al, 2006Raju et al, , 2008Barroso-Chinea et al, 2008) enabled us to address this issue directly. We thus performed a quantitative analysis of the connectivity of the two glutamatergic afferents in relation to the subpopulations of MSNs.…”

Section: Introductionmentioning

confidence: 99%

Cortical and Thalamic Innervation of Direct and Indirect Pathway Medium-Sized Spiny Neurons in Mouse Striatum

Doig¹,

Moss²,

Bolam³

2010

J. Neurosci.

203

173

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The striatum receives major excitatory inputs from the cortex and thalamus that predominantly target the spines of medium-sized spiny neurons (MSNs). We aimed to determine whether there is any selectivity of these two excitatory afferents in their innervation of direct and indirect pathway MSNs. To address this, we used bacterial artificial chromosome transgenic mice, in which enhanced green fluorescent protein (EGFP) reports the presence of D 1 or D 2 dopamine receptor subtypes, markers of direct and indirect pathway MSNs, respectively. Excitatory afferents were identified by the selective expression of vesicular glutamate transporter type 1 (VGluT1) by corticostriatal afferents and vesicular glutamate transporter type 2 (VGluT2) by thalamostriatal afferents. A quantitative electron microscopic analysis was performed on striatal tissue from D 1 and D 2 mice that was double immunolabeled to reveal the EGFP and VGluT1 or VGluT2. We found that the proportion of synapses formed by terminals derived from the cortex and thalamus was similar for both direct and indirect pathway MSNs. Furthermore, qualitative analysis revealed that individual cortical or thalamic terminals form synapses with both direct and indirect pathway MSNs. Similarly, we observed a convergence of cortical and thalamic inputs onto individual MSNs of both direct and indirect pathway: individual EGFP-positive structures received input from both VGluT2-positive and VGluT2-negative terminals. These findings demonstrate that direct and indirect pathway MSNs are similarly innervated by cortical and thalamic afferents; both projections are thus likely to be critical in the control of MSNs and hence play fundamental roles in the expression of basal ganglia function.

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“…On the other hand, the ventral thalamic nuclei are the source of a somewhat smaller number of thalamic striatal innervation. The neurons involved co-express both types of VGluTs (Barroso-Chinea et al, 2008a). Indeed, panels E-H of Fig.…”

Section: Resultsmentioning

confidence: 84%

“…1 show that all neurons located in the ventral lateral posterior nucleus of the thalamus (VPL) coexpress VGluT1 and VGluT2 mRNA, and that a subpopulation of neurons within VPL innervates the striatum. Moreover, it is also worth noting that both mRNAs share a complementary distribution at the subcellular level (panel 1H, see also Barroso-Chinea et al, 2008a). Dual fluorescence ISH for VGluT1 and VGluT2 mRNAs can be combined with retrograde tracing as illustrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…However, non-radioactive ISH (i.e., fluorescence based) is more reproducible than radioactive ISH and a lesser risky to both researcher and the environment. Combination of fluorescence ISH with fluorescence retrograde tracing is easy, and an additional advantage of fluorescence methods is the highly efficient and reliable analysis by means of confocal laser scanning microscopy (Hur and Zaborszky, 2005;Pérez-Manso et al, 2006;Barroso-Chinea et al, 2007, 2008aWouterlood et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Neuroanatomical tracing combined with in situ hybridization: Analysis of gene expression patterns within brain circuits of interest

Conte-Perales

Barroso‐Chinea

Rico

et al. 2010

Journal of Neuroscience Methods

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Expression of vesicular glutamate transporters 1 and 2 in the cells of origin of the rat thalamostriatal pathway

Cited by 47 publications

References 25 publications

Immunohistochemical localization of vesicular glutamate transporter 2 (vGluT2) in the central nervous system of the pigeon (Columba livia)

Immunohistochemical localization of vesicular glutamate transporter 2 (vGluT2) in the central nervous system of the pigeon (Columba livia)

Cortical and Thalamic Innervation of Direct and Indirect Pathway Medium-Sized Spiny Neurons in Mouse Striatum

Neuroanatomical tracing combined with in situ hybridization: Analysis of gene expression patterns within brain circuits of interest

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