Expression of transmembrane 4 superfamily (TM4SF) proteins and their role in hepatic stellate cell motility and wound healing migration

Mazzocca, Antonio; Carloni, Vinicio; Sciammetta, Silvia Cappadona; Cordella, Claudia; Pantaleo, Pietro; Caldini, Anna; Geñtilini, Paolo; Pinzani, Massimo

doi:10.1016/s0168-8278(02)00175-7

Cited by 62 publications

(44 citation statements)

References 33 publications

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“…Interestingly, genes included in the proposed predictor are not cancer genes or genes encoding elements of the adhesion system, migration or proteolysis, but rather suggest branching signal transduction pathways with possible extensive networks between individual pathways and between cells themselves. For example, in the nonneoplastic mucosa of patients who recurred, we have observed an overexpression of two membrane receptors, annexin 2 and transmembrane protein 4, previously shown to be involved in tumor invasion (Mazzocca et al, 2002;Hashida et al, 2003;Tanaka et al, 2004). Conversely, in the mucosa of patients who did not recur after a follow-up of 5 years, we have observed an increased expression of some genes already reported to induce tumor cell invasion: basigin, known to stimulate production of matrix metalloproteinases by fibroblasts, a member of transmembrane protein 4 and CD24 (Kanekura et al, 2002;Kristiansen et al, 2004).…”

Section: Discussionmentioning

confidence: 80%

Prognosis of stage II colon cancer by non-neoplastic mucosa gene expression profiling

et al. 2006

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We have assessed the possibility to build a prognosis predictor (PP), based on non-neoplastic mucosa microarray gene expression measures, for stage II colon cancer patients. Non-neoplastic colonic mucosa mRNA samples from 24 patients (10 with a metachronous metastasis, 14 with no recurrence) were profiled using the Affymetrix HGU133A GeneChip. Patients were repeatedly and randomly divided into 1000 training sets (TSs) of size 16 and validation sets (VS) of size 8. For each TS/VS split, a 70-gene PP, identified on the TS by selecting the 70 most differentially expressed genes and applying diagonal linear discriminant analysis, was used to predict the prognoses of VS patients. Mean prognosis prediction performances of the 70-gene PP were 81.8% for accuracy, 73.0% for sensitivity and 87.1% for specificity. Informative genes suggested branching signal-transduction pathways with possible extensive networks between individual pathways. They also included genes coding for proteins involved in immune surveillance. In conclusion, our study suggests that one can build an accurate PP for stage II colon cancer patients, based on non-neoplastic mucosa microarray gene expression measures.

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Section: Discussionmentioning

confidence: 80%

Prognosis of stage II colon cancer by non-neoplastic mucosa gene expression profiling

et al. 2006

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“…Furthermore, earlier studies by Recinos et al (12) using microarray analysis of total liver RNA have indicated that feeding mice a high-fat diet results in significant changes in the expression of hepatic genes involved in cholesterol metabolism as well as in the expression of CD68 and CD63. The latter two proteins are expressed in Kupffer and stellate cells (13,14) but not in parenchymal cells, which together with our findings (10,11) suggests that it is difficult to interpret data from microarray studies that are based on total liver mRNA. Therefore, in this study, using microarray technology, we focused on the specific response of liver parenchymal cells to atherogenic diet feeding in LDL receptor-deficient mice, an established atherosclerosis mouse model.…”

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confidence: 60%

Microarray analysis indicates an important role for FABP5 and putative novel FABPs on a Western-type diet

Hoekstra

Stitzinger

Wanrooij

et al. 2006

Journal of Lipid Research

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Liver parenchymal cells play a dominant role in hepatic metabolism and thereby total body cholesterol homeostasis. To gain insight into the specific pathways and genes involved in the response of liver parenchymal cells to increased dietary lipid levels under atherogenic conditions, changes in parenchymal cell gene expression upon feeding a Western-type diet for 0, 2, 4, and 6 weeks were determined using microarray analysis in LDL receptor-deficient mice, an established atherosclerotic animal model. Using ABI Mouse Genome Survey Arrays, we were able to detect 7,507 genes (28% of the total number on an array) that were expressed in parenchymal cells isolated from livers of LDL receptordeficient mice at every time point investigated. Timedependent gene expression profiling identified fatty acid binding protein 5 (FABP5) and four novel FABP5-like transcripts located on chromosomes 2, 8, and 18 as important proteins in the primary response of liver parenchymal cells to Western-type diet feeding, because their expression was 16-to 22-fold increased within the first 2 weeks on the Westerntype diet. The rapid substantial increase in gene expression suggests that these FABPs may play an important role in the primary protection against the cellular toxicity of cholesterol, free fatty acids, and/or lipid oxidants. Furthermore, as a secondary response to the Western-type diet, liver parenchymal cells of LDL receptor-deficient mice stimulated glycolysis and lipogenesis pathways, resulting in a steady, more atherogenic serum lipoprotein profile (increased VLDL/LDL). High levels of circulating cholesterol attributable to the consumption of Western-type/high-fat diets form a major risk factor for atherosclerosis and subsequent cardiovascular diseases (e.g., myocardial infarction, stroke) (1), which are the leading causes of death in the Western world. Several mutations in the LDL receptor are associated with familial hypercholesterolemia, a dominantly inherited error of metabolism characterized by increased plasma LDL levels, xanthomas of skin and tendons, and premature heart disease caused by atherosclerosis of the coronary arteries (2).The liver is an essential organ in the regulation of serum cholesterol levels because it is able to clear excess cholesterol from the blood for subsequent excretion into the bile (3, 4). In addition, the liver is responsible for the synthesis and secretion of VLDL and HDL, respectively (5, 6). Because of the important role of the liver in the control of serum cholesterol levels, several studies have recently been conducted using microarray technology to determine the molecular mechanisms underlying long-term high-fat dietinduced alterations in total mouse liver (7-9). However, a common problem with these types of microarray studies is the heterogeneity of the liver, which contains several different cell types, each of which has its specific localization and function. Kupffer cells are tissue macrophages strategically located within the liver sinusoids, and their function is the remova...

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“…Tetraspanins, a large family of ubiquitously expressed membrane proteins, have been identified and implicated in the regulation of cell development, differentiation, proliferation, motility and tumor cell invasion (19)(20)(21). In many human cancers, tumor progression was found to be associated with an altered expression of tetraspanins (22).…”

Section: Discussionmentioning

confidence: 99%

Tetraspanin 1 is involved in survival, proliferation and carcinogenesis of pancreatic cancer

et al. 2015

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Abstract. Pancreatic cancer (PCC) is one of the most difficult cancers to treat and the 10th leading cause of cancer-related death in worldwide. Studies have demonstrated that the tetraspanin 1 (Tspan1) is overexpressed in various cancers and may be a potential therapeutic strategy for the treatment of different cancers. However, the possible role of Tspan1 in PCC is still unknown. In the present study, our data revealed that the increased Tspan1 in PCC tissues was associated with the clinicopathological features and survival rate of PCC patient. We also investigated the effects of Tspan1 gene knockdown on the biological behavior of human PCC. The expression of Tspan1 (detected by immunohistochemistry, qRT-PCR and western blot analysis) derived from human PCC tissues and cell lines (AsPC-1 and PANC-1), were significantly elevated compared with those of the control (P<0.05). Transfection with siRNA-targeting Tspan1 significantly decreased proliferation, increased the apoptosis and reduced migration and invasion of AsPC-1 and PANC-1 cells. The present study demonstrated that Tspan1 plays an important role in PCC carcinogenic progression, including migration and invasion. The siRNA targeting of Tspan1 may be a potential therapeutic strategy for the treatment of PCC.

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Expression of transmembrane 4 superfamily (TM4SF) proteins and their role in hepatic stellate cell motility and wound healing migration

Cited by 62 publications

References 33 publications

Prognosis of stage II colon cancer by non-neoplastic mucosa gene expression profiling

Prognosis of stage II colon cancer by non-neoplastic mucosa gene expression profiling

Microarray analysis indicates an important role for FABP5 and putative novel FABPs on a Western-type diet

Tetraspanin 1 is involved in survival, proliferation and carcinogenesis of pancreatic cancer

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