Expression of thrombomodulin in squamous cell carcinoma of the lung: its relationship to lymph node metastasis and prognosis of the patients

Ogawa, Hiroki; Yonezawa, Suguru; Maruyama, Ikuro; Matsushita, Yosuke; Tezuka, Yoshihisa; Toyoyama, Hironobu; Yanagi, Masakazu; Matsumoto, Hajime; Nishijima, Hironobu; Shimotakahara, T; Aikou, Takashi; Satô, Eiichi

doi:10.1016/s0304-3835(99)00348-1

Cited by 47 publications

(41 citation statements)

References 20 publications

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“…The percentage positive staining of thrombomodulin in both UCa and primary pulmonary SCC is consistent with previously published data, although the percentage positivity of thrombomodulin expression in the present study is slightly higher. 7,[17][18][19][20] Neither antibodies against napsin A nor antibodies against surfactant protein A were found to be helpful for distinguishing these 2 tumor types, as both failed to show significant expression of either protein. Expression levels of napsin A and surfactant protein A detected in the primary pulmonary SCC cases from the present study are in agreement with results published by Ueno et al 21 While the immunohistochemical data from the series of paired tumors could not definitively identify the presence of synchronous primary urothelial and primary pulmo- nary lesions among the 30-patient cohort, two of the lung lesions from the paired case series displayed an immunophenotype more similar to that of the typical primary pulmonary squamous cell carcinoma than the typical primary invasive bladder urothelial carcinoma in our series (case 8 and case 23; Table 4).…”

Section: Commentmentioning

confidence: 99%

Selective Immunohistochemical Markers to Distinguish Between Metastatic High-Grade Urothelial Carcinoma and Primary Poorly Differentiated Invasive Squamous Cell Carcinoma of the Lung

Gruver

Amin

Luthringer

et al. 2012

Archives of Pathology &Amp; Laboratory Medicine

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N Context.-Distinction between primary lung carcinomas and metastases from other sites, especially the urinary tract, is a common diagnostic dilemma. As urothelial carcinomas can demonstrate a broad range of morphology and frequently demonstrate squamous differentiation, discerning metastatic urothelial carcinoma to the lung from primary pulmonary squamous cell carcinoma can be challenging.Objective.-To investigate immunostains that may aid in the distinction of urothelial carcinoma metastatic to the lung.Design.-Staining patterns of 14 markers in primary urothelial carcinoma of the bladder and primary squamous cell carcinoma of the lung were examined to establish a diagnostic panel. These antibodies were subsequently tested on tumors taken from 30 patients with a paired urinary tract and metastatic lung lesion.Results.-The best markers to distinguish poorly differentiated metastatic urothelial carcinoma from primary pulmonary squamous cell carcinoma were CK7, CK20, GATA-3, CK14, desmoglein-3, and uroplakin III, with the utility of the latter dependent upon the quantity of tissue available for analysis. The observed percentage positive staining in nonmetastatic urothelial carcinoma versus primary pulmonary squamous cell carcinoma with these antibodies was as follows: CK7 (100% versus 33%), CK20 (54% versus 7%), GATA-3 (78% versus 23%), CK14 (32% versus 77%), desmoglein-3 (11% versus 87%), and uroplakin III (14% versus 0%). Similar expression patterns were observed among the paired cases.Conclusion.-When interpreted in correlation with clinical history and histomorphology, a panel of immunostains including CK7, CK20, GATA-3, CK14, desmoglein-3, and uroplakin III may be a useful adjunct in the distinction of metastatic urothelial carcinoma to the lung.

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Section: Commentmentioning

confidence: 99%

Selective Immunohistochemical Markers to Distinguish Between Metastatic High-Grade Urothelial Carcinoma and Primary Poorly Differentiated Invasive Squamous Cell Carcinoma of the Lung

Gruver

Amin

Luthringer

et al. 2012

Archives of Pathology &Amp; Laboratory Medicine

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“…TM expression has been immunohistochemically demonstrated in a wide variety of human tumours, (Tezuka et al, 1995;Hamatake et al, 1996;Kim et al, 1997;Ordonez, 1997;Tabata et al, 1997;Wilhelm et al, 1998;Zhang et al, 1998;Ogawa et al, 2000), which mostly reveal a correlation between reduced TM expression and shorter survival or increased metastases (Hamatake et al, 1996;Kim et al, 1997;Tabata et al, 1997;Ogawa et al, 2000).…”

mentioning

confidence: 99%

“…It has also been demonstrated to colocalize with actin filaments (Huang et al, 2003) suggesting an involvement in intercellular communication or (cell -cell) adhesion. Mirroring other cell-adhesion molecules, TM expression tends to be lower in metastatic lesions than in matched primary specimens (Tezuka et al, 1995;Hamatake et al, 1996;Tabata et al, 1997;Ogawa et al, 2000).…”

mentioning

confidence: 99%

Thrombomodulin expression in colorectal carcinoma is protective and correlates with survival

et al. 2006

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Thrombomodulin (TM) is an endothelial receptor that exhibits anticoagulant, antifibrinolytic and anti-inflammatory activity by inhibiting thrombin and cellular adhesion. In this study, the expression and significance of TM was examined in primary colorectal cancer and its prognostic implications explored. TM immunostaining was performed on formalin-fixed, paraffin-embedded tissue sections, from primary lesions of 200 patients with colorectal carcinoma. Institutional Ethical approval was granted and clinical data retrieved from patients' records. All normal colonic tissue expressed TM on endothelial cells. TM tumour cell expression was demonstrated in 53 (26.5%) cases and 147 (73.5%) showed no neoplastic cell staining. On univariate and multivariate analysis TM expression on tumour cells correlated significantly with tumour stage, differentiation, Jass score and 5 year survival. TM expression decreases as overall stage and tumour size increase (P ¼ 0.03). In all, 91% TM positive tumours were well differentiated and 85% of TM negative tumours were poorly differentiated (Po0.01). Five year survival rates of patients with positive and negative TM expression were 71 and 41%, respectively. Survival rate was poorer in those patients who were TM negative compared with those who were positive (Po0.01). A total of 101 (50.5%) of the cases were node negative. In this group, 5 year survival rates of patients with positive and negative TM expression were 87.5 and 37.8%, respectively, demonstrating a poorer survival rate for those who are node negative and TM negative at the time of surgery (Po0.001). This study demonstrates that loss of TM is a key indicator in tumour biology and prognosis.

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“…The tumors induced by the TMG-expressed cells have the smallest size in comparison with other transfected cells. DISCUSSION TM, which is a well known anticoagulation factor, may function as a cell adhesion molecule, given that the glycoprotein is present in the junction of different epithelial cells (7,8,19). This role for TM was investigated in the present study.…”

Section: Expression Of Tmg and Tmg(⌬l) Proteins In A2058 Cells-mentioning

confidence: 95%

“…The lectin-like activity may be influential in a cell-to-cell adhesive interaction (19). It is conceivable that TM may function as an additional cellular adhesive molecule.…”

Section: Thrombomodulin (Tm)mentioning

confidence: 99%

Thrombomodulin-mediated Cell Adhesion

Huang

Shi

Jiang

et al. 2003

Journal of Biological Chemistry

138

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Expression of thrombomodulin in squamous cell carcinoma of the lung: its relationship to lymph node metastasis and prognosis of the patients

Cited by 47 publications

References 20 publications

Selective Immunohistochemical Markers to Distinguish Between Metastatic High-Grade Urothelial Carcinoma and Primary Poorly Differentiated Invasive Squamous Cell Carcinoma of the Lung

Selective Immunohistochemical Markers to Distinguish Between Metastatic High-Grade Urothelial Carcinoma and Primary Poorly Differentiated Invasive Squamous Cell Carcinoma of the Lung

Thrombomodulin expression in colorectal carcinoma is protective and correlates with survival

Thrombomodulin-mediated Cell Adhesion

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