Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells

Förster, Reinhold; Emrich, Thomas; Kremmer, Elisabeth; Lipp, Martin

doi:10.1182/blood.v84.3.830.bloodjournal843830

Cited by 228 publications

(131 citation statements)

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“…In support of this notion, a consistent finding has been the ability of RANTES and MCP-1 specifically to attract memoryT cells [7,13,141. In addition, the BLR-1 chemokine receptor-like molecule is expressed on a subset of memory T cells [29]. This suggests that chemokine receptor expression on T cells is regulated during the course of T cell differentiation or activation.…”

Section: Expression Of Il-8ra and Il-8rb On Blood T Cells 4 Discussionmentioning

confidence: 99%

Expression of monocyte chemoattractant protein‐1 and interleukin‐8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential

et al. 1996

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The differential expression of chemokine receptors may be an important mechanism for the regulation of T cell migration. To test this, we examined the expression and function of the monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8 receptors on various population of T cells. Using a simple and reliable transendothelial chemotaxis assay, both MCP-1 and IL-8 were shown to be chemotactic for subsets of blood T cells, although the relative response varied from donor to donor. To examine receptor expression and correlate it with chemotaxis of T cell subsets, monoclonal antibodies (mAb) to the receptors were produced by immunizing mice either with synthetic peptides (MCP-1 receptor), or with receptor transfectants (IL-8 receptors A and B). A flow cytometric analysis of blood T cells with an anti-MCP-1 receptor mAb revealed low expression on the CD26hi subset and undetectable expression on other T cells. Staining of T cells with anti-Il-8RA and anti-IL-8RB showed much higher levels of expression, but only on a subset of CD3+ cells which were CD8+ and CD56+. That IL-8 and MCP-1 attracted distinct subsets of T cells was best illustrated using the CD26 marker, since IL-8R+ T cells were CD26-, whereas T cells expressing detectable MCP-1R or which responded to MCP-1 in chemotaxis assays were CD26hi. T cells activated in vitro with anti-CD3 up-regulated expression of the MCP-1 receptor, but not the IL-8 receptors, and were attracted to MCP-1 much more efficiently than resting T cells. These results show that there is a clear distinction between the IL-8 and MCP-1-responsive T cell populations and that chemokine receptor expression on T cells may be regulated with respect to linkage as well as cellular activation.

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Section: Expression Of Il-8ra and Il-8rb On Blood T Cells 4 Discussionmentioning

confidence: 99%

Expression of monocyte chemoattractant protein‐1 and interleukin‐8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential

et al. 1996

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“…The chemokine receptor CXCR5 is highly expressed on mature, recirculating B cells, subsets of CD4 + and CD8 + T cells, as well as DCs (12,48,66). CXCL13, the only known ligand for CXCR5, is exclusively expressed within the B-cell zones of secondary lymphoid organs, where it is produced by stromal cells including follicular dendritic cells (FDCs) (18).…”

mentioning

confidence: 99%

Shaping Up Adaptive Immunity: The Impact of CCR7 and CXCR5 on Lymphocyte Trafficking

Müller

Lipp

2003

Microcirculation

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The effective onset of adaptive immune responses requires that naïve antigen-specific lymphocytes, being inherently rare throughout the body, rapidly encounter foreign antigens. This problem has been elegantly solved in evolution by inventing secondary lymphoid tissues as intersections in the migratory pathway of antigen-presenting dendritic cells and antigen-specific lymphocytes. Chemokines play a central role in guiding cell movements in the course of immune responses and in lymphoid system homeostasis. In particular, the chemokine receptors CCR7 and CXCR5 are key molecules for the entry of lymphocytes and dendritic cells into secondary lymphoid organs and their homing to T-cell and B-cell zones therein. CCR7 and CXCR5 are differentially expressed on the cell surface of lymphocytes and dendritic cells depending on the stage of cellular differentiation and activation, thus allowing these cells to change their homing capacity and prospective traffic routes.

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“…CXCL13 is a member of CXC chemokines without glutamic acid-leucine-arginine motif. Consistent with a general rule that CXC chemokines lacking this motif are chemoattractants for lymphocytes with an anti-angiogenic property, [9] CXCL13 induces chemotaxis of mature B cells, follicular helper T (Tfh) cells, T helper (Th) 17 cells and regulatory T cells (Tregs) through its receptor CXCR5, [10][11][12][13][14] while inhibiting angiogenesis by interfering with basic fibroblast growth factor (bFGF). [15] In lymphoid tissues, CXCL13 protein is detected in macrophages, follicular dendritic cells and endothelial cells, but it is produced solely from macrophages among these three cell types.…”

Section: Introductionmentioning

confidence: 93%

CXCL13 produced by macrophages due to Fli1 deficiency may contribute to the development of tissue fibrosis, vasculopathy and immune activation in systemic sclerosis

Taniguchi

Miyagawa

Toyama

et al. 2018

Experimental Dermatology

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CXCL13, a chemokine for B cells, follicular T cells, T helper 17 cells, and regulatory T cells, is reported to contribute to the development of systemic sclerosis (SSc), reflecting aberrant activation of immune system. To better understand the role of CXCL13 in SSc, we investigated the influence of Fli1 deficiency, a potential predisposing factor of this disease, on CXCL13 expression and assessed the clinical correlation of serum CXCL13 levels by multivariate regression analysis. Haploinsufficient loss of Fli1 remarkably induced CXCL13 expression in murine peritoneal macrophages, while gene silencing of FLI1 did not affect the expression of CXCL13 in human dermal fibroblasts and human dermal microvascular endothelial cells. Serum CXCL13 levels were elevated in SSc patients compared with healthy controls and correlated positively with skin score and negatively with pulmonary function test results. SSc patients with elevated serum CXCL13 levels had longer disease duration, diffuse cutaneous involvement, interstitial lung disease (ILD), heart involvement, pulmonary arterial hypertension, Raynaud's phenomenon, pitting scars, digital ulcers, telangiectasia, and high serum IgG levels more frequently than the other patients. In particular, serum CXCL13 levels were associated with ILD and digital ulcers by multivariate regression analysis. Taken together, these results indicate that CXCL13 expression is upregulated by Fli1 deficiency in macrophages, potentially contributing to the development of tissue fibrosis, vasculopathy and immune activation in SSc, especially ILD and digital ulcers.

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Expression of the G-protein--coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells

Cited by 228 publications

References 0 publications

Expression of monocyte chemoattractant protein‐1 and interleukin‐8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential

Expression of monocyte chemoattractant protein‐1 and interleukin‐8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential

Shaping Up Adaptive Immunity: The Impact of CCR7 and CXCR5 on Lymphocyte Trafficking

CXCL13 produced by macrophages due to Fli1 deficiency may contribute to the development of tissue fibrosis, vasculopathy and immune activation in systemic sclerosis

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