Expression of soluble vascular endothelial growth factor receptor-1 and placental growth factor in fetal growth restriction cases and intervention effect of tetramethylpyrazine

Li, Shuwei; Liu, Yi; Song, Jihyun; Lin, Yan; Chen, Ze-Jun; Hu, Chunxia; Wang, Minghua; Yao, Mao-Zhong

doi:10.1016/s1995-7645(14)60112-7

Cited by 13 publications

(7 citation statements)

References 6 publications

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“…The herb consists of many kinds of chemical constituents, including TMP, volatile oil, and ferulic acid. A survey of literatures shows that TMP [23,24] and some lactones [25] in the extract of L. chuanxiong have effects of anti-atherosclerosis and antiangiogenesis. The major pathologic mechanisms of the two ailments involve capillary endothelial cell proliferation, migration, and tissue infiltration [26,27].…”

Section: Resultsmentioning

confidence: 99%

Screening bioactive compounds from Ligusticum chuanxiong by high density immobilized human umbilical vein endothelial cells

Wang

Liu

et al. 2015

Anal Bioanal Chem

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High throughput screening methodologies play a very important role in screening bioactive compounds from complex media. In this work, a new strategy for attaching cells onto amino microspheres using human umbilical vein endothelial cells (HUVECs) as a probe was developed. The immobilization depended on the specific affinity between integrin on the cells and the RGD peptide, which was coated on poly[oligo (ethylene glycol) methacrylate] by atom transfer radical polymerization. Validated application of the stationary phase was performed in the analysis of Ligusticum chuanxiong extraction by high performance affinity chromatography-mass spectrometry. Three compounds were screened as the bioactive compounds of Ligusticum chuanxiong. Two of them were identified as 3-butyl-hexahydroisobenzofuran-1(3H)-one and tetramethylpyrazine (TMP), whereas the other one remains indistinct. The association constant of vascular endothelial growth factor (VEGF) and TMP binding to VEGF receptor (VEGFR) on HUVECs were calculated to be (1.04 ± 0.08) × 10(11) M(-1) and (9.84 ± 1.11) × 10(8) M(-1) by zonal elution. Molecular docking showed that one hydrogen bond was formed between N atom of TMP and 3-N atom of imidazole group in histidine(223) of VEGFR. Both zonal elution and molecular docking indicated that TMP and VEGF bind to the same site of VEGFR on HUVECs. It is possible to become a promising tool for high throughput screening of the bioactive compounds binding to HUVECs through broad application of the stationary phase.

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Section: Resultsmentioning

confidence: 99%

Screening bioactive compounds from Ligusticum chuanxiong by high density immobilized human umbilical vein endothelial cells

Wang

Liu

et al. 2015

Anal Bioanal Chem

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“…PLGF: this is the placenta analog of VEGF; it has pro-inflammatory and angiogenic properties 43. In animal models, mRNA PLGF level has decreased in both serum and placenta in intrauterine growth restriction (IUGR) 44. Through immunohistochemistry, PLGF has been reported expressed in syncytiotrophoblast and endothelial cells of the fetal capillaries in human placentas;45 however, we found its expression in more cells: extravillous trophoblast, decidua, chorionic stromal cells, basal trophoblast, in addition to syncytiotrophoblast and endothelial cells of the fetal capillaries.…”

Section: Discussionmentioning

confidence: 99%

“…We also found expression in the UC cells (described earlier). Comparing normal and abnormal placentas, PLGF has been reported to be normal in serum and as increased in placental tissue in cases of placental dysfunction (IUGR and PE);44 with Elisa, others have reported it as more significantly decreased in preeclamptic placentas than in normotensive ones 45. Our study found that the absence of PLGF expression in stromal cells could be a protector for HDP; on the contrary, it appears to be a risk factor when it is absent or scant in the syncytiotrophoblast and in the UC (endothelium and smooth muscle cells).…”

Section: Discussionmentioning

confidence: 99%

The umbilical cord, preeclampsia and the VEGF family

Contreras

Garrido

Hernández-Losa

et al. 2018

IJWH

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IntroductionThe VEGF family has been identified as abnormal in preeclampsia (PE). Hypertensive disorders of pregnancy (HDP) are major contributors to maternal and neonatal morbidity and mortality worldwide; likewise, umbilical cord anatomical abnormalities (UCAA) are linked to poor neonatal outcomes. Based on the relationship described between PE and UCAA and the role of the VEGF family in PE, this study explored VEGF expression in placental and UC tissued from patients with PE and with UCAA.MethodsWe performed an observational, analytical study on placentas, comparing protein and mRNA expression in four groups: patients with PE, patients with UC abnormalities, patients with both, and patients with none of them. Using immunohistochemistry, we studied VEGF A, VEGF R1 (FLT1), MMP1, and PLGF. With quantitative reverse transcription polymerase chain reaction we described mRNA expression of PLGF, VEGF and sFLT1, and sFLT1/PLGF ratio.ResultsForty newborns were included. Sixty-seven percent of mothers and 45% of newborns developed no complications. Immunohistochemistry was performed on UC and placental disc paraffin-embedded tissue; in the latter, the mRNA of the VEGF family was also measured. Statistically significant differences were observed among different expressions in both HDP and UCAA groups. Interestingly, the UCAA group exhibited lower levels of sFLT1 and VEGF-A in comparison with other groups, with significant P-value for sFLT1 (P=0000.1).ConclusionThe origin of UCAA abnormalities and their relation with HDP are still unknown. VEGF family alterations could be involved in both. This study provides the first approach related to molecules linked to UCAA.

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“…The application of artificial oxygen carriers, a kind of hemoglobin vesicles, which are nano-scale sized and blood-type antigens free, in a rat preeclampsia model induced by continuous administration of the NO synthetase inhibitor was demonstrated to reduce the hypoxic status of the placenta and fetus by supplying oxygen through the pathological narrow spiral arteries . A set of vascular-relaxation agents, including antioxidant 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (Tempol) (Stanley et al 2012), Ligustrazine (Li et al 2014b), Resveratrol (Poudel et al 2013), and Sildenafil citrate (Dilworth et al 2013), ameliorated blood rheological status and increased fetal weight in different FGR animal models (Fig. 1).…”

Section: Animal Models and Experimental Intervention In Fgrmentioning

confidence: 99%

Progress in the understanding of the etiology and predictability of fetal growth restriction

Tang

Liu

et al. 2017

Reproduction

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Fetal growth restriction (FGR) is defined as the failure of fetus to reach its growth potential for various reasons, leading to multiple perinatal complications and adult diseases of fetal origins. Shallow extravillous trophoblast (EVT) invasion-induced placental insufficiency and placental dysfunction are considered the main reasons for idiopathic FGR. In this review, first we discuss the major characteristics of anti-angiogenic state and the pro-inflammatory bias in FGR. We then elaborate major abnormalities in placental insufficiency at molecular levels, including the interaction between decidual leukocytes and EVT, alteration of miRNA expression and imprinted gene expression pattern in FGR. Finally, we review current animal models used in FGR, an experimental intervention based on animal models and the progress of predictive biomarker studies in FGR. Free Chinese abstract: A Chinese translation of this abstract is freely available at http://www.reproduction-online.org/content/153/6/ R227/suppl/DC1. Reproduction (2017) 153 R227-R240

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Expression of soluble vascular endothelial growth factor receptor-1 and placental growth factor in fetal growth restriction cases and intervention effect of tetramethylpyrazine

Cited by 13 publications

References 6 publications

Screening bioactive compounds from Ligusticum chuanxiong by high density immobilized human umbilical vein endothelial cells

Screening bioactive compounds from Ligusticum chuanxiong by high density immobilized human umbilical vein endothelial cells

The umbilical cord, preeclampsia and the VEGF family

Progress in the understanding of the etiology and predictability of fetal growth restriction

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