Expression of SHH signaling molecules in the developing human primary dentition

Hu, Xuefeng; Zhang, Shuo; Chen, Guimiao; Lin, Chensheng; Huang, Zhen; Zhang, Yanding

doi:10.1186/1471-213x-13-11

Cited by 28 publications

(22 citation statements)

References 29 publications

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“…In humans, however, this process takes about 400 days from the initiation stage at embryonic week 6 to tooth eruption at postnatal month 6 (Zhang et al 2005). Our previous (Dong et al 2014;Hu et al 2013Huang et al 2014;Lin et al 2007) and current studies support the idea that morphogenetic diversity of tooth development may result from tinkering with the conserved signaling during evolution.…”

Section: The Differences Between Human and Mouse Tooth Morphology Corsupporting

confidence: 72%

“…It is conceivable that the differences in human and mouse tooth development process could be a consequence of the differential expression of FGF4, FGF7, FGF8, and FGF9, because they are continuously expressed in the differentiating ameloblasts and odontoblasts at the differentiation stage. In addition to FGFs, multiple families of signaling molecules are also expressed in the developing human tooth, including SHH, WNTs, and BMPs (Dong et al 2014;Hu et al 2013;Lin et al 2007;Wang et al 2014). These genes also exhibited similar but slightly distinct expression patterns in the developing human tooth as compared to their counterparts in the mouse.…”

Section: The Differences Between Human and Mouse Tooth Morphology Cormentioning

confidence: 98%

“…Written consent was obtained from participants for the use of their gestation-terminated fetuses as research materials according to the procedure permitted by the Ethics Committee of Fujian Normal University. Crown-rump length (CRL) was used to estimate precise gestational age (Hu et al 2013). …”

Section: Human Embryosmentioning

confidence: 99%

“…Human cDNA templates for RNA probes, including , were purchased from Open Biosystems (Thermo Scientific Inc) and were subcloned to pBluescript KS + vector, respectively, for DIG-labeled antisense riboprobe transcription using DIG RNA Labeling Kit (Roche). Sections in situ hybridization were performed with standard procedures (Hu et al 2013). For immunohistochemistry, the deparaffinized and serum-blocked sections were incubated with primary antibodies according to the manufacturer's instruction.…”

Section: In Situ Hybridization and Immunochemical Stainingmentioning

confidence: 99%

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Expression profile of critical genes involved in FGF signaling pathway in the developing human primary dentition

Huang

Chun-ni

et al. 2015

Histochem Cell Biol

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Mammalian tooth development is regulated by paracrine signal molecules of several conserved family interactions between epithelium and mesenchyme. The expression patterns and regulative roles of FGF signaling have been extensively studied in the mouse odontogenesis; however, that is not well known in human tooth development. In order to unveil the molecular mechanisms that regulate human tooth morphogenesis, we examined the expression patterns of the critical molecules involved in FGF signaling pathway in the developing human tooth germ by in situ hybridization, immunohistochemistry, and real-time RT-PCR, including FGF ligands, receptors, and intracellular transducer. We found overlapping but distinct expression pattern of FGF ligands and receptors in the different stages and components. Expression of FGF4, FGF7, FGF8, and FGF9 persists widespread in human tooth mesenchyme, which is quite different to that of in mouse. FGFR1 may be the major receptor in regulate mechanisms of FGF signals in human tooth development. Real-time RT-PCR indeed confirmed the results of in situ hybridization. Results of K-Ras, p-ERK1/2, p-p38, p-JNK, and p-PDK1 expression reveal spatial and temporal patterns of FGF signaling during morphogenesis and organogenesis of human tooth germ. Activity of the FGF signaling transducer protein in human tooth germ was much higher than that of in mouse. Our results provided important FGF singling information in the developing process, pinpoint to the domains where the downstream target genes of FGF signaling can be sought, and enlightened our knowledge about the nature of FGF signaling in human tooth germ.

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Section: The Differences Between Human and Mouse Tooth Morphology Corsupporting

confidence: 72%

Section: The Differences Between Human and Mouse Tooth Morphology Cormentioning

confidence: 98%

Section: Human Embryosmentioning

confidence: 99%

Section: In Situ Hybridization and Immunochemical Stainingmentioning

confidence: 99%

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Expression profile of critical genes involved in FGF signaling pathway in the developing human primary dentition

Huang

Chun-ni

et al. 2015

Histochem Cell Biol

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“…gene expression profile and their function during human odontogenesis. Although the developmental processes and the expression patterns of some genes share many similarities between human and mouse developing tooth germs, there are genes that exhibit distinct expression patterns in the developing tooth of humans and mice (Hu et al 2013;Lin et al 2007;Wang et al 2014). In order to establish a comprehensive gene expression profile in the developing human tooth and to gain a better understanding of the molecular basis of human dental abnormalities, we conducted a genome-wide analysis of gene expression by microarray assay using the cap stage human embryonic tooth germ and identified a number of genes that are preferentially expressed in the tooth germ by comparing the expression profile with that from the embryonic lip tissues.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of gene expression in human embryonic tooth germ

Huang

Lin

et al. 2014

J Mol Hist

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While numerous genes that play important regulatory roles during tooth development in mice have been identified, little is known about gene expression profile and their function during human odontogenesis. To unveil expression profile of odontogenic genes in humans, we conducted genome-wide gene expression analysis by microarray assays to analyze differential gene expression between tooth germ and lip tissue from 11-week old human fetuses. We identified 167 genes that are strongly expressed in the cap stage tooth germ as compared to the lip tissue. Among them, 145 genes were further identified by gene ontology enrichment analysis that are highly represented in multiple gene ontology classes, include extracellular components, sequence-specific DNA binding proteins, Wnt-protein binding molecules, system development, organogenesis, and cell differentiation. Sixty-seven genes that are known to be associated with mammalian tooth development and tooth abnormalities were identified. Real-time PCR was further employed to validate microarray data. Moreover, in situ hybridization assay demonstrated tooth type specific expression of ISL1 and BARX1 in the incisor, canine, and molar respectively, consistent with microarray results. Our results represent a set of reliable data that could provide a solid base for future elaboration of molecular mechanisms underlying human tooth development.

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Expression patterns of genes critical for BMP signaling pathway in developing human primary tooth germs

Dong

Shen

Ruan

et al. 2014

Histochem Cell Biol

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The developing murine tooth has been used as an excellent model system to study the molecular mechanism of organ development and regeneration. While the expression patterns of numerous regulatory genes have been examined and their roles have begun to be revealed in the developing murine tooth, little is known about gene expression and function in human tooth development. In order to unveil the molecular mechanisms that regulate human tooth morphogenesis, we examined the expression patterns of the major BMP signaling pathway molecules in the developing human tooth germ at the cap and bell stages by in situ hybridization, immunohistochemistry, and real-time RT-PCR. Expression of BMP ligands and antagonist, including BMP2, BMP3, BMP4, BMP7, and NOOGGIN, exhibited uniform patterns in the tooth germs of incisor and molar at the cap and bell stages with stronger expression in the inner dental epithelium than that in the dental mesenchyme. Both type I and type II BMP receptors were present in widespread expression pattern in the whole-enamel organ and the dental mesenchyme with the strongest expression in inner dental epithelium at the cap and bell stages. SMAD4 and SMAD1/5/8 showed an expression pattern similar to that of BMP ligands with more intensive signals in the inner dental epithelium. Despite some unique and distinct patterns as compared to the mouse, the intensive expression of BMP signaling pathway molecules in the developing human tooth strongly suggests conserved functions of BMP signaling during human odontogenesis, such as in mediating tissue interactions and regulating differentiation and organization of odontogenic tissues. Our results provide an important set of documents for studying molecular regulatory mechanisms underlying tooth development and regeneration in humans.

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Expression of SHH signaling molecules in the developing human primary dentition

Cited by 28 publications

References 29 publications

Expression profile of critical genes involved in FGF signaling pathway in the developing human primary dentition

Expression profile of critical genes involved in FGF signaling pathway in the developing human primary dentition

Genome-wide analysis of gene expression in human embryonic tooth germ

Expression patterns of genes critical for BMP signaling pathway in developing human primary tooth germs

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