Expression of seven-in-absentia homologue 1 and hypoxia-inducible factor 1 alpha: Novel prognostic factors of nasopharyngeal carcinoma

Kitagawa, Noriko; Kondo, Satoru; Wakisaka, Naohiro; Zen, Yoh; Nakanishi, Yosuke; Tsuji, Akira; Endo, Kazuhira; Murono, Shigeyuki; Yoshizaki, Tomokazu

doi:10.1016/j.canlet.2012.12.002

Cited by 33 publications

(35 citation statements)

References 28 publications

(37 reference statements)

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“…However, these factors have limited value in determining low and high risk groups and the treatment modality. In recent years, the search for novel prognostic factors that more reliably predict the biological behavior of the tumors has focused on the role of the various molecular biomarkers (Acikalin et al, 2012;Kitagawa et al, 2013). In this study, we focused on the expression of NR4A2 in NPC and its relationship with the clinicopathologic parameters and prognosis of the disease.…”

Section: Discussionmentioning

confidence: 99%

High Cytoplasmic Expression of the Orphan Nuclear Receptor NR4A2 Predicts Poor Survival in Nasopharyngeal Carcinoma

Wang

Yang²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Section: Discussionmentioning

confidence: 99%

High Cytoplasmic Expression of the Orphan Nuclear Receptor NR4A2 Predicts Poor Survival in Nasopharyngeal Carcinoma

Wang

Yang²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Paraffin-embedded specimens were used for the immunohistochemical analysis of NDRG1 (using anti-NDRG1 antibody; Cell Signaling, catalog no. 9485), as well as for EBER-ISH, as previously described (47).…”

Section: Immunohistochemistry and Eber-ish Of Npc Biopsy Specimensmentioning

confidence: 99%

Clustered MicroRNAs of the Epstein-Barr Virus Cooperatively Downregulate an Epithelial Cell-Specific Metastasis Suppressor

Kanda

Miyata

Kano

et al. 2015

J Virol

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The Epstein-Barr virus (EBV) encodes its own microRNAs (miRNAs);Although in vitro reporter assays identified BART22 as being responsible for the NDRG1 downregulation, EBV genetic analyses revealed that BART22 was not solely responsible; rather, the entire BART miRNA cluster 2 was responsible for the downregulation. Immunohistochemical analyses revealed that the expression level of the NDRG1 protein was downregulated significantly in EBV-positive nasopharyngeal carcinoma specimens. Considering that NDRG1 encodes an epithelial differentiation marker and a suppressor of metastasis, these data implicate a causative relationship between BART miRNA expression and epithelial carcinogenesis in vivo. IMPORTANCEEBV-related epithelial cancers, such as nasopharyngeal carcinomas and EBV-positive gastric cancers, encompass more than 80% of EBV-related malignancies. Although it is known that they express high levels of virally encoded BART miRNAs, how these miRNAs contribute to EBV-mediated epithelial carcinogenesis remains unknown. Although a number of screenings have been performed to identify targets of viral miRNAs, many targets likely have not been identified, especially in case of epithelial cell infection. This is the first study to use EBV genetics to perform unbiased screens of cellular genes that are differentially expressed in viral miRNA-positive and -negative epithelial cells. The result indicates that multiple EBV-encoded miRNAs cooperatively downregulate NDRG1, an epithelial differentiation marker and suppressor of metastasis. The experimental system described in this study should be useful for further clarifying the mechanism of EBV-mediated epithelial carcinogenesis. T he Epstein-Barr virus (EBV) is a common herpesvirus that is widespread in all human populations. Primary EBV infections in adolescence often manifest as infectious mononucleosis (1).EBV infection is also associated with several types of lymphomas and epithelial malignancies. The B95-8 strain of EBV, an infectious mononucleosis-derived isolate, is biologically indistinguishable from other isolates of EBV in that it efficiently produces progeny virus and transforms peripheral B-lymphocytes in vitro (2). The marmoset lymphoblastoid B95-8 cell line is one of the most widely used EBV-producing cell lines. Although the B95-8 strain was assumed to be a prototype EBV, restriction mapping and DNA sequencing analyses revealed that its genome contains a deletion of approximately 12 kb (3-5). This deleted region is apparently dispensable for progeny virus production and B-cell transformation; hence, its importance has been underestimated.The recent discovery of EBV-encoded microRNA (miRNA) genes within the 12-kb region has dramatically changed the situation. The initial discovery of five EBV miRNAs was followed by the subsequent identification of a number of additional miRNAs (6-9); to date, 44 mature miRNAs have been identified, of which 4 are encoded at the BHRF1 locus, and 40 are encoded at the BART locus. A complete list of EBV miRNAs, includin...

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“…La expresión de LMP-1 es más uniforme en etapas tempranas del CNF y en carcinoma in situ, lo que sugiere que esta proteína puede facilitar la progresión de la enfermedad (49), debido a que concentraciones bajas de la proteína son suficientes para inducir cambios morfológicos y fenotipos invasivos en células de CNF in vitro (50,51). LMP-1 contribuye a la inmortalización de células B y a la transformación y proliferación de fibroblastos (in vivo) y de ciertas líneas de células epiteliales (in vitro) (52), promueve la motilidad, la invasión, las metásta-sis (53,54), la transición epitelio-mesenquimal (55) y la angiogénesis (56,57).…”

Section: Veb Y Carcinoma Nasofaríngeo (Cnf)unclassified

Virus de Epstein-Barr y su relación con el desarrollo del cáncer

Medina-Ortega

López-Valencia

Mosquera-Monje

et al. 2017

iatreia

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RESUMENEl virus de Epstein-Barr (VEB) es un agente infeccioso que tiene tropismo por células linfoides y ocasionalmente por células epiteliales. La Agencia Internacional para la Investigación sobre Cáncer (IARC, por su sigla en inglés) lo clasificó hace 20 años como carcinógeno de tipo I, porque durante la infección latente expresa diferentes proteínas o micro-ARN con capacidad oncogénica, por lo que las células infectadas tendrían el potencial de desarrollar cáncer. Esto se ha demostrado en algunos tipos de cáncer como linfomas, carcinoma nasofaríngeo y cán-cer gástrico, mientras que la asociación no es completamente clara en los cánceres de mama y pulmón. La presente revisión describe, profundiza y analiza la relación del VEB con dichos tipos de cáncer, así como los métodos diagnósticos empleados para su detección. Finalmente, se plantean preguntas cuyas respuestas podrían contribuir al conocimiento de los mecanismos moleculares involucrados en la relación VEB-cáncer. PALABRAS CLAVE

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Expression of seven-in-absentia homologue 1 and hypoxia-inducible factor 1 alpha: Novel prognostic factors of nasopharyngeal carcinoma

Cited by 33 publications

References 28 publications

High Cytoplasmic Expression of the Orphan Nuclear Receptor NR4A2 Predicts Poor Survival in Nasopharyngeal Carcinoma

High Cytoplasmic Expression of the Orphan Nuclear Receptor NR4A2 Predicts Poor Survival in Nasopharyngeal Carcinoma

Clustered MicroRNAs of the Epstein-Barr Virus Cooperatively Downregulate an Epithelial Cell-Specific Metastasis Suppressor

Virus de Epstein-Barr y su relación con el desarrollo del cáncer

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