Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

Song, Qingqing; Deng, Xun; Song, Ruiqing

doi:10.3390/microorganisms8040601

Cited by 28 publications

(10 citation statements)

References 37 publications

(39 reference statements)

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“…In another study on sugar cane bagasse and straw, the LC from Pycnoporus cinnabarinus in the presence of HBT caused a noticeable delignification, which correlates with a substantial improvement of saccharification yields. Recombinant LC from Pleurotus ostreatus was shown to be able to degrade corn stover lignin in the presence of suitable mediators . Also a bacterial LC from Bacillus ligniniphilus , alone or in the presence of mediators such as ABTS, has proven effective in lignin (alkaline lignin and milled wood lignin) oxidative degradation, as it has been recently shown.…”

Section: Delignification Main Strategies and Protocolsmentioning

confidence: 88%

“…Recombinant LC from Pleurotus ostreatus was shown to be able to degrade corn stover lignin in the presence of suitable mediators. 156 Also a bacterial LC from Bacillus ligniniphilus, alone or in the presence of mediators such as ABTS, has proven effective 157 in lignin (alkaline lignin and milled wood lignin) oxidative degradation, as it has been recently shown. In particular, a selective removal of S units, within the frame of general demethoxylation, was observed.…”

Section: Protocolsmentioning

confidence: 97%

See 1 more Smart Citation

Recent Developments in the Delignification and Exploitation of Grass Lignocellulosic Biomass

Tocco

Carucci

Monduzzi

et al. 2021

ACS Sustainable Chem. Eng.

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Biofuels could be defined as the fuels of the future, although much work is still required before they will replace fossil fuels. In this review, lignocellulosic biomass based on straw and related crops and wastes is described concerning its lignin contents, structure, and properties, and an overview on the means of current and predictable delignification protocols is presented. The discussion is focused on herbaceous monocot materials and their available pretreatments (physical, chemical, and enzymatic), with special emphasis on fungal ligninolytic enzymes and the most recent findings and developments in their current application, issues, and perspectives.

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Section: Delignification Main Strategies and Protocolsmentioning

confidence: 88%

Section: Protocolsmentioning

confidence: 97%

Recent Developments in the Delignification and Exploitation of Grass Lignocellulosic Biomass

Tocco

Carucci

Monduzzi

et al. 2021

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…Laccases contain four copper ions except for the laccase that is obtained from Phlebia radiata, which has only two copper ions [245]. There are three types of Lac depending on the copper number at the active site [246]. Type I: copper does not bind O 2 but functions only as an electron transfer site.…”

Section: Lignin Degradation Enzymesmentioning

confidence: 99%

“…Most of the studies on Lac have reported that the fungi and mushrooms present in basidiomycetes, deuteromycetes and ascomycetes act as Lac producers [247]. Among these fungi, the major Lac producers are white-rot fungi in basidiomycetes [246]. White-rot fungi Pycnoporus cinnabarinus, Phlebia radiate, P. ostreatus, and T. versicolour are also known to produce one isoform of Lac [248].…”

Section: Lignin Degradation Enzymesmentioning

confidence: 99%

Cultivation of Mushrooms and Their Lignocellulolytic Enzyme Production Through the Utilization of Agro-Industrial Waste

et al. 2020

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A large amount of agro-industrial waste is produced worldwide in various agricultural sectors and by different food industries. The disposal and burning of this waste have created major global environmental problems. Agro-industrial waste mainly consists of cellulose, hemicellulose and lignin, all of which are collectively defined as lignocellulosic materials. This waste can serve as a suitable substrate in the solid-state fermentation process involving mushrooms. Mushrooms degrade lignocellulosic substrates through lignocellulosic enzyme production and utilize the degraded products to produce their fruiting bodies. Therefore, mushroom cultivation can be considered a prominent biotechnological process for the reduction and valorization of agro-industrial waste. Such waste is generated as a result of the eco-friendly conversion of low-value by-products into new resources that can be used to produce value-added products. Here, we have produced a brief review of the current findings through an overview of recently published literature. This overview has focused on the use of agro-industrial waste as a growth substrate for mushroom cultivation and lignocellulolytic enzyme production.

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“…48424 LAC48424-1 P. pastoris [ 164 ] Basidiomycota T. versicolor LCC1 g.e Yarrowia lipolytica [ 165 ] Basidiomycota T. versicolor LCCA, LCCB, P. pastoris [ 166 , 167 ] Basidiomycota Trametes sp. 5930 LAC5930-1 P. pastoris [ 168 ] Basidiomycota T. versicolor Lac6c S. cerevisiae EBY100 [ 169 ] Basidiomycota Lenzites gibbosa LAC P. pastoris [ 164 ] Basidiomycota Pleurotus ostreatus POXA1b g.e S. cerevisiae [ 169 ] Basidiomycota P. pulmonarius (PpuLcc) PpuLcc P. pastoris GS115 [ 170 ] Basidiomycota Pycnoporus sanguineus LCC1 P. pastoris [ 171 ] Basidiomycota P. ostreatus Lac-2 P. pastoris X33 [ …”

Section: Synthetic Biology In Fungo : Gene Editingmentioning

confidence: 99%

Genome-based engineering of ligninolytic enzymes in fungi

et al. 2021

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Background Many fungi grow as saprobic organisms and obtain nutrients from a wide range of dead organic materials. Among saprobes, fungal species that grow on wood or in polluted environments have evolved prolific mechanisms for the production of degrading compounds, such as ligninolytic enzymes. These enzymes include arrays of intense redox-potential oxidoreductase, such as laccase, catalase, and peroxidases. The ability to produce ligninolytic enzymes makes a variety of fungal species suitable for application in many industries, including the production of biofuels and antibiotics, bioremediation, and biomedical application as biosensors. However, fungal ligninolytic enzymes are produced naturally in small quantities that may not meet the industrial or market demands. Over the last decade, combined synthetic biology and computational designs have yielded significant results in enhancing the synthesis of natural compounds in fungi. Main body of the abstract In this review, we gave insights into different protein engineering methods, including rational, semi-rational, and directed evolution approaches that have been employed to enhance the production of some important ligninolytic enzymes in fungi. We described the role of metabolic pathway engineering to optimize the synthesis of chemical compounds of interest in various fields. We highlighted synthetic biology novel techniques for biosynthetic gene cluster (BGC) activation in fungo and heterologous reconstruction of BGC in microbial cells. We also discussed in detail some recombinant ligninolytic enzymes that have been successfully enhanced and expressed in different heterologous hosts. Finally, we described recent advance in CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR associated) protein systems as the most promising biotechnology for large-scale production of ligninolytic enzymes. Short conclusion Aggregation, expression, and regulation of ligninolytic enzymes in fungi require very complex procedures with many interfering factors. Synthetic and computational biology strategies, as explained in this review, are powerful tools that can be combined to solve these puzzles. These integrated strategies can lead to the production of enzymes with special abilities, such as wide substrate specifications, thermo-stability, tolerance to long time storage, and stability in different substrate conditions, such as pH and nutrients.

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Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

Cited by 28 publications

References 37 publications

Recent Developments in the Delignification and Exploitation of Grass Lignocellulosic Biomass

Recent Developments in the Delignification and Exploitation of Grass Lignocellulosic Biomass

Cultivation of Mushrooms and Their Lignocellulolytic Enzyme Production Through the Utilization of Agro-Industrial Waste

Genome-based engineering of ligninolytic enzymes in fungi

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