Expression of P16 biomarker in squamous cell carcinoma of uterine cervix and its association with clinico-pathological parameters: A cross-sectional study

Raju, Kalyani; Raghuveer, C. V.; Sheela, S R

doi:10.15419/bmrat.v7i9.626

Cited by 4 publications

(1 citation statement)

References 17 publications

(31 reference statements)

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“…In the present study, IHC P16 expression in terms of diffuse block positive, focal nuclear positive or negative expression showed block positivity in 22 cases (44.0 %), focal nuclear in 18 cases (36.0%) and negativity in 10 cases (20.0 %); with overall 80% p16 positive and 20% p16 negative. Our results agreed with Sarwath et al [15] and Kaylani et al [16] trials, where anti-p16 ink4a IHC correlation in SCC and other cervical tumors showed positive expression in 92% and 89% respectively.…”

Section: Discussionsupporting

confidence: 93%

Prognostic Impact of Human Papilloma Virus Infection on Cervical Cancer Patients Reflected by p16ink4a Expression: Single Institution Experience

Ismail

Gaballah

Salama

et al. 2023

Asian Pac J Cancer Biol

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Background: Human Papilloma virus (HPV) is the main cause of cervical cancer. Infection with HPV can be reflected by overexpression of p16ink4a. Aim of the study: we aimed at evaluating the expression of p16ink4a in cervical cancer patients and its prognostic significance. Patients and methods: This retrospective registry and follow up study was conducted on 95 women diagnosed with cervical cancer. After screening of patients presented to our hospitals; 50 patients were eligible for inclusion in the study. P16ink4a was assessed by immunohistochemistry on archived tumor’s samples and correlation with different epidemiological, clinical and pathological data was performed. Prognostic impact of P16ink4a on overall survival (OS) and event free survival (EFS) was evaluated.Results: Median age of patients was 55 years. The main presenting symptom was bleeding. Most of the patients presented with late FIGO staging 64% with stage IIIb and 10% with stage IIb. P16ink4a was positive in 80% of patients. Correlation between P16ink4a and different clinic-epidemiological data revealed positive significant correlation with tumor grade and tumor size (P values of 0.03 and 0.05 respectively). Considering the effect of p16ink4a expression and EFS and OS, our study failed to show any significant correlation.Conclusion: The late stage at presentation of our population encourages the need for national screening program. The main cause of cervical cancer is HPV reflected by positive P16 similar to international literature. In our study the failure to reach a significant correlation with survival may be due the small sample size.

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Section: Discussionsupporting

confidence: 93%

Prognostic Impact of Human Papilloma Virus Infection on Cervical Cancer Patients Reflected by p16ink4a Expression: Single Institution Experience

Ismail

Gaballah

Salama

et al. 2023

Asian Pac J Cancer Biol

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Immunohistochemical expression of P16 and Ki-67 in uterine cervical neoplasms

Zabin¹,

Akhtar

Mohsin³

et al. 2021

JDPO

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To study the expression of Ki-67 and p16 in neoplastic lesions of uterine cervix and to evaluate the prognostic significance of tumour differentiation, histological type, stage and grade, depth of tumour invasion and lymphovascular invasion in women with neoplasia of uterine cervix. This study was performed on 50 biopsies and surgically resected specimens of uterine cervix, which were fixed in 10% formalin, processed, paraffin embedded and cut into 3-5 microns thickness, stained with hematoxylin and eosin stains and immunohistochemical staining by p16 and Ki-67 antibodies was performed and studied. Majority of cases of invasive carcinoma cervix were large cell non-keratinizing squamous cell carcinoma, 58 cases (48.2%). Majority of cases of invasive carcinoma cervix were seen in stage 2B, 40 (40.8%) cases followed by stage 3B, 24 (24.4%) cases. Out of 18 cases of large cell non-keratinizing squamous cell carcinoma, 12 (66.7%) showed 3+ positivity for Ki-67. 7 (53.8%) cases of stage 2A showed 3+ positivity, 3 (23.1%) cases each showed 2+ positivity for p16. 7 (36.8%) cases of moderately differentiated SCC showed 3+ positivity, 4 (21.1%) showed 2+ positivity and 5 (26.3%) showed 1+ positivity of Ki-67. Out of 10 stable patients, 4 (40.0%) showed negative p16 immunoexpression and all the 3 cases (100%) with recurrence showed 2+ positivity and 2 (100%) patients with metastasis showed 3+ positivity. Out of 10 stable patients, 6 (60%) showed negative Ki-67 immunoexpression and all the 3 (100%) cases with recurrence showed 3+ positivity and one each (50.0%) patient with metastasis showed 2+ positivity and 3+ positivity. p16 and Ki-67 immunomarkers are useful as a diagnostic and prognostic tool in cases with recurrence and metastasis, helping in early detection of disease progression.

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P16 and EGFR analyses in cervical carcinoma on paraffin material in Egyptian females: the NCI experience

Badawy,

Loay,

Negmaldin

2023

EGJP

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Introduction Cervical cancer is considered the fourth most prevalent malignant tumor in women worldwide. In Egypt, it ranks as the 14th most frequent cancer among women. Epidermal growth factor receptor has been an attractive target for anticancer therapy. Although EGFR mutations are less commonly seen in cervical cancer, there is a potential correlation between EGFR expression level and clinical response. Overexpression of p16INK4a is associated with increased overall and disease-free survival in cervical cancer, thus denoting a better prognosis. Aim of the work To investigate the expression of EGFR and p16 by immunohistochemistry as well as amplification of EGFR by FISH technique in cervical carcinoma among Egyptian females. Material and methods Seventy-seven cases of invasive cervical carcinomas were retrieved from paraffin blocks. EGFR and p16 expression were assessed by immunohistochemistry. EGFR gene amplification was assessed by FISH analysis. Results p 16 Positivity was detected in 62.3% of cases. Positive reaction to EGFR (score 3+) was detected in 20.8% of cases. The EGFR gene was not amplified in 77.9% of cases. Statistical significance was found between histological type, p16, and EGFR expression. A fair agreement was detected between EGFR expression and EGFR amplification. Poor agreement was detected between p16, EGFR expression, and EGFR amplification. Conclusion EGFR and p16 have an independent role in the development of cervical cancer in Egyptian females. EGFR overexpression and EGFR amplification represent two different genetic events; in other words, an increase in gene copy number does not mean that there will be an increase in the function of the gene as expressed by an increase in protein expression.

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Expression of P16 biomarker in squamous cell carcinoma of uterine cervix and its association with clinico-pathological parameters: A cross-sectional study

Cited by 4 publications

References 17 publications

Prognostic Impact of Human Papilloma Virus Infection on Cervical Cancer Patients Reflected by p16ink4a Expression: Single Institution Experience

Prognostic Impact of Human Papilloma Virus Infection on Cervical Cancer Patients Reflected by p16ink4a Expression: Single Institution Experience

Immunohistochemical expression of P16 and Ki-67 in uterine cervical neoplasms

P16 and EGFR analyses in cervical carcinoma on paraffin material in Egyptian females: the NCI experience

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