Expression of Nestin and IGF‐1 in Rat Pancreas after Streptozotocin Administration

Abstract

The present study examines whether centroacinar (CA) and intercalated duct (ICD) cells can serve as stem cells, after administration of the diabetogenic agent streptozotocin (STZ). Thirty rats were divided into five experimental groups: (1) control, (2) 1 day after STZ (STZ-1), (3) 3 days after STZ (STZ-3), (4) 7 days after STZ (STZ-7) and (5) 14 days after STZ (STZ-14). Many small pancreatic islets were observed in the STZ-7 group than in the other experimental groups, and many of these small islets were in close contact with ICD and CA cells. A higher number of nestin, insulin-like growth factor-1 (IGF-1) and IGF-1-receptor positive ICD and CA cells were observed at STZ-3 and STZ-7 than at the others. These expression patterns coincided well with the proliferating cell nuclear antigen pattern. The results suggest that rat pancreatic endocrine cells after damage by STZ administration might be recovered from newly generated cells derived from ICD and CA cells.

Expression of transcription factors and precursor cell markers during regeneration of β cells in pancreata of rats treated with streptozotocin

“…Surprisingly, however, there was no nestin expression at all. This observation contrasts with the results of Nagasao et al [10] in STZ-treated neonatal rats. They found that 1% of the cells were nestin-positive 3 and 7 days after administration of STZ.…”

“…We expected to find a rise in expression of these transcription factors in the cells of all pancreatic compartments in association with concomitant c-Kit (CD117) and nestin expression. These two markers have been reported to be associated with pancreatic ducts and islets during pancreatic regeneration [6,10,12,21]. Finally, we wanted to gain insights into the different modes of regeneration in the pancreas of STZ-treated diabetic rats in comparison with other models.…”

Identificação de genes diferencialmente expressos durante diferenciação de células-tronco e caracterização de células progenitoras mesenquimais

“…Após a diferenciação, as células produziam insulina, glucagon, GLP-1 e PDX-1 (Zulewski et al, 2001). A nestina, no entanto, não está presente somente no pâncreas endócrino, mas também nos ductos (Nagasao et al, 2004). Estas célulastronco nestina-positivas também têm características de precursores de células β, com altos níveis de PDX-1 Nagasao et al, 2004).…”

“…A nestina, no entanto, não está presente somente no pâncreas endócrino, mas também nos ductos (Nagasao et al, 2004). Estas célulastronco nestina-positivas também têm características de precursores de células β, com altos níveis de PDX-1 Nagasao et al, 2004). Infelizmente, os estudos com células nestina-positivas foram descontinuados porque foi mostrado, em alguns estudos, que a nestina no pâncreas é expressa somente em células que não estão destinadas a tornarem-se células β (Treutelaar et al, 2003;Delacour et al, 2004;Street et al, 2004).…”