Expression of MMP-9 in different degrees of chronic hepatitis B and its correlation with inflammation

Abstract

Expression of matrix metalloproteinase-9 (MMP-9) in different degrees of chronic hepatitis B (CHB) and the correlation of MMP-9 with inflammation was investigated. A total of 96 CHB patients (observation group) admitted and treated in Dongying People's Hospital from December 2016 to November 2017 were selected, and they were compared with 60 healthy individuals (control group) selected in the physical examination center during the same time period. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of MMP-9, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), MMP-9 expression in different inflammation grades and fibrosis stages, and the relationship between MMP-9 and inflammation was analyzed. The levels of MMP-9, TNF-α and IL-6 in serum in the observation group were obviously higher than those in the control group (P<0.05). The rank sum test indicated that there were statistically significant differences in the expression levels of MMP-9 among the inflammation grades G0, G1, G2, G3 and G4 (P<0.05). The expression levels of MMP-9 in fibrosis stages S0, S1, S2, S3 and S4 were detected via the rank sum test, and it suggested that the differences among the 5 stages were statistically significant (P<0.05). Pearsons correlation analysis showed that the MMP-9 expression level was positively correlated with TNF-α and IL-6 (P<0.05). In conclusion, the MMP-9 level is elevated remarkably in patients with varying degrees of CHB, it may play an important role in the pathological progress of liver, and it has a close correlation with inflammation, which can provide a theoretical basis for clinical treatment.

Matrix metalloproteinase-9 -1562 C/T polymorphism is associated with the risk of sepsis in a Chinese population: A retrospective study

“…MMP-9 is shown to regulate inflammation in different diseases. 10 – 12 Meanwhile, MMP-9 also remodels the extracellular matrix and controls the activity of numerous growth factors, cytokines, cell adhesion molecules and chemokines, which are essential in inflammation. 13 , 14 In sepsis, MMP-9 is associated with increased vascular permeability, which promotes inflammatory cell migration and regulates the crucial inflammatory response.…”

A functional MMP-9-1562C>T polymorphism, MMP-9 serum levels and nephrolithiasis risk in a southern Chinese population

“…Frontiers in Medicine 05 frontiersin.org (23)(24)(25)(26). A link between MMP-9 and extracellular signal-regulated kinase (ERK) can be found in Schwann cells, and ERK involves in renal interstitial fibrosis, which is thought to be the final result of CKD (27).…”

Suppression of Interferon-α Treatment Response by Host Negative Factors in Hepatitis B Virus Infection

“…IFNAR1 degradation can be promoted by either ligand binding and cellular signaling induced ubiquitination-dependent pathway, or Ser532 phosphorylation and p38 kinase activity requiring ligand-independent way ( 69 ). MMP-9 can be activated by HBV in CHB patients ( 70 , 71 ) and in turn facilitates HBV replication through attenuating IFN/JAK/STAT signaling, interacting with IFNAR1 to promoting its phosphorylation, ubiquitination, subcellular distribution, and degradation of IFNAR1 through lysosome pathways in Ser532 phosphorylation and p38 independent manner, as well as blocking its binding to IFN-α ( 39 ). However, a recent study found type I IFN strongly promotes the clearance of MMPs ( 72 ), thus, further study is needed to investigate the role of MMPs in the IFN-α therapy mediated antiviral efficacy against HBV infection.…”