Expression of matrix metalloproteinase-1 in round ligament and uterosacral ligament tissue from women with pelvic organ prolapse

Abstract

To evaluate the matrix metalloproteinase-1 (MMP-1) expression in different parts of pelvic connective tissue in postmenopausal women with and without pelvic organ prolapse (POP). Ninety-one samples were obtained from only postmenopausal women (42 with POP and 49 non-POP subjects). All women were evaluated by pelvic organ prolapse quantitation. The POP group had stage 2 or more, and the controls had stage 1 or less. Round ligament (RL) and uterosacral ligament (USL) biopsies were obtained from women with POP and controls. Immunohistochemistry for MMP-1 was performed on formalin-fixed and paraffin-embedded sections. The two groups were matched for age, body mass index, parity and postmenopausal status. MedCalc Statistical Software Programme Version 12.0.5 was used for statistical analysis. Expression of MMP-1 were significantly higher in both RL and USL tissue from postmenopausal women with POP, compared with controls. MMP-1 immunoreactivities were identified in both RL and USL biopsies from all women with and without POP. The expression pattern of MMP-1 were similar in these ligaments and were significantly higher in POP group compared with control subjects. These changes indicate a possible relation between MMP-1 expression of RL and USL in women with and without POP.

Matrix Metalloproteinase‐1 Expression in Women With and Without Pelvic Organ Prolapse: A Systematic Review and Meta‐analysis

“…The sample sizes of the cases with POP ranged from 20 to 46, and those of the controls were from 20 to 49. In four of the studies, cases and controls were all postmenopausal, and in one investigation 85% of the POP cases and 20% of the controls were postmenopausal. The mean age ranged from 55 to 61.4 years for the cases with POP and from 49.7 to 59 for the controls.…”

“…Thus, we included both of them . Consequently, five examinations were included in our meta‐analysis, which contained 182 POP cases and 192 controls. A flow chart of the data collection process is presented in Figure .…”

“…The basic characteristics of the included studies are presented in Table , containing the name of the first author, year of publication, country of origin, ethnicity of the study population, age and menopausal status of cases and controls, staining method, source of control, sample size, and number of cases and controls in each staining level. Out of these investigations, three were conducted in Croatia, one in Israel, and one in Turkey . The sample sizes of the cases with POP ranged from 20 to 46, and those of the controls were from 20 to 49.…”

“…Several investigations evaluated the expression of MMP‐1 in different parts of the supportive pelvic structures in women with and without POP . However, the results are not completely consistent.…”

Modern molecular genetic and biochemical predictors of genital prolapse (a review)

“…Выявлено, что у пациенток с ПГ в круглых и крестцово-маточных связках значительно повышена экспрессия ММР-1. Это приводит к ускоренному метаболизму и распаду матрикса в подвешивающем и фиксирующем аппа-рате матки [31]. Также доказано, что у пациенток с ПГ уровень сывороточной ММР-10 выше по сравне-нию со здоровыми женщинами.…”