Expression of macrophage‐selective markers in human and rodent adipocytes

Abstract

CD14, CD68 and/or mouse F4/80 or human epidermal growth factor module-containing mucin-like receptor 1 (EMR1) are widely used as macrophage-specific markers. Since macrophages infiltrate several tissues during inflammatory processes, CD14, CD68 and EMR1-F4/80 have been employed to discriminate between tissue-containing macrophages, like adipose tissue (AT), and other cells. Using real-time PCR experiments, we show that isolated adipocytes from humans and mice AT express high levels of CD14 and CD68 mRNA, whereas EMR1-F4/80 is mainly present in the macrophage-containing stroma-vascular fraction. Furthermore, fibroblasts-like cells (adipoblasts), preadipocytes and adipocytes from the murine cell lines, 3T3-F442A and BFC-1, express CD14 and CD68 mRNA and protein as determined by fluorescence-activated cell sorter, but not F4/80 which, as expected, is strongly expressed in the macrophage cell line RAW264.7. These results reinforce the view that EMR1-F4/80 is the best macrophage marker to date and show that CD14 and CD68 are not macrophage-specific proteins.

Cellular Plasticity of Inflammatory Myeloid Cells in the Peritoneal Foreign Body Response

“…Although CD11b is expressed by a number of cell types including monocyte/ macrophages, granulocytes, and lymphocytes, 25,26 F4/80 is considered the most specific macrophage marker. 12,13 Consistent with their identity as resident peritoneal macrophages, the majority of EGFP ϩ cells within the unstimulated peritoneal cavity stained for both CD11b (myeloid cells) ( Figure 3B) and F4/80 (macrophages) ( Figure 3C). The EGFP Ϫ subset never expressed F4/80, whereas a small proportion of these cells did express CD11b and were most likely lymphocytes.…”

“…The mononuclear phagocyte system encompasses bone marrow precursors, peripheral blood monocytes, tissue macrophages, and dendritic cells, all of which express the macrophage colony-stimulating factor receptor (csf1r). 9 -11 Macrophages also express F4/80 12,13 and exhibit phenotypic and functional heterogeneity (reviewed in 14). Recently, blood monocytes have also been shown to exhibit heterogeneity in terms of expression of surface molecules such as Gr1 (Ly6C), chemokine receptors (CX3CR1), and migratory predisposition.…”

Continuous cold exposure induces an anti-inflammatory response in mesenteric adipose tissue associated with catecholamine production and thermogenin expression in rats

“…One or 7 days cold exposure induced rise in β3-ARs mRNA (both p<0.05). In order to assess whether cold exposure may stimulate an alternative activation of the mWAT tissue macrophages by interleukin 4 (IL-4) leading to activation of thermogenic gene expression as described by Nguyen et al (2011), we determined the expression of macrophages markers F4/80 and CD68 (Khazen et al 2005) and expression of the pro-infl ammatory and anti-infl ammatory factors in the mWAT (Figure 2). One or 7 days cold exposure stimulated the infi ltration of macrophages demonstrated by elevated of F4/80 (both p<0.05) and CD68 (both p<0.05) expression.…”

Macrophages regulate tumor necrosis factor-α expression in adipocytes through the secretion of matrix metalloproteinase-3

“…High-fat intake induced the expression of MMP-3 in mesenteric fat tissues as well as the induction of F4/80 gene To assess the expression of MMP-3 gene in adipose tissue Mf, the levels of MMP-3 mRNA were examined in mesenteric fat tissue from mice fed with high-fat diet in relation to the expression of the F4/80 gene, an Mf-specific antigen 15,20 ( Figure 6). High-fat intake for 2 weeks significantly induced the expression level of F4/80 mRNA in mesenteric fat tissue by 2.2-fold in comparison to the level in the control mice.…”