Expression of Intestinal Trefoil Factor in Developing Rat Intestine

Lin, Jing; Holzman, Ian R.; Jiang, Ping; Babyatsky, Mark W.

doi:10.1159/000014146

Cited by 40 publications

(18 citation statements)

References 15 publications

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“…An upregulated expression of TFF3 around the weaning period was measured in rat intestine [44]. This is concordant with our results in piglets, showing a 2-fold increased TFF3 expression in distal jejunum and ileum comparing specific pre-and postweaning time points.…”

Section: Discussionsupporting

confidence: 91%

Intestinal Expression of TFF and Related Genes During Postnatal Development in a Piglet Probiotic Trial

Scholven

Taras²,

Sharbati

et al. 2009

Cell Physiol Biochem

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Trefoil factor family (TFF) peptides provide protective and reparative effects by enhancing epithelial integrity and promoting mucosal restitution. TFF peptide expression is induced after mucosal damage. These processes are of central physiological relevance during the postnatal intestinal development and are strongly influenced during the weaning period. In piglets, weaning at early maturation stages frequently causes mucosal inflammation. The aim of this study was to evaluate postnatal intestinal TFF expression in a piglet probiotic trial. Low intestinal TFF2 expression was measured at early maturation stages. Weaning, however, was associated with a distinct response of increased TFF2 expression, indicating an important role in enhancing mucosal integrity. In the distal jejunum and ileum weaning could as well be associated with increased TFF3 mRNA levels. Differential TFF1 expression was not detected. Furthermore, TFF2 localization studies in different intestinal loci were performed by means of immunohistochemistry. Expression of selected genes (TGFA, EGFR, Cox-2) known to promote TFF signaling showed differential expression pattern as well, thereby providing further functional background. Furthermore, the expression patterns of EGFR observed in this study contribute to an advanced view of previous findings of EGFR regulation mainly obtained in rodents. An upregulated EGFR expression during early postnatal development suggests a local relevance to porcine intestinal maturation. However, a feed supplementation with the probiotic strain Enterococcus faecium did not influence TFF expression.

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Section: Discussionsupporting

confidence: 91%

Intestinal Expression of TFF and Related Genes During Postnatal Development in a Piglet Probiotic Trial

Scholven

Taras²,

Sharbati

et al. 2009

Cell Physiol Biochem

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“…In addition, we provide evidence to suggest that D12 may be a critical period as striking changes occurred with regard to the gene expression of mucins and Tff3 in the proximal and distal colon of normal pups. After weaning, we found a sharp increase in the mRNA and protein expression of Muc2, Muc4, and Tff3 in the proximal colon of normal rat, as previously observed (28,34,35). These changes in the expression of mucus constituents were parallel to morphologic changes of the mucosa (increase in crypt depth and in the number of goblet cells per crypt, reduction in the percentage of crypts in fission) that were consistent with previous studies (36 -38) and revealed a maturation of rat colonic mucosa.…”

Section: Discussionsupporting

confidence: 87%

Intrauterine Growth Restriction Alters Postnatal Colonic Barrier Maturation in Rats

et al. 2009

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Intrauterine growth restriction (IUGR) is a leading cause of perinatal mortality and morbidity and increases the risk for necrotizing enterocolitis. We hypothesized that colonic barrier disruption could be responsible for intestinal frailty in infants and adults born with IUGR. Mucins and trefoil factor family 3 (TFF3) actively contribute to epithelium protection and healing. Our aim was to determine whether IUGR affects colonic mucosa maturation. IUGR was induced by dietary protein restriction in pregnant dams. Mucins and Tff3 expression and morphologic maturation of the colonic mucosa were followed during postnatal development of the offspring. Before weaning, mucin 2 and Tff3 protein levels were reduced in colonic mucosa of rats with IUGR compared with controls. After weaning, expression of mucin 2 (mRNA and protein) and mucin 4 (mRNA) were lower in colonic mucosa of rats with IUGR. At the same time, IUGR was associated with a reduction of crypt depth and a higher percentage of crypts in fission. We conclude that IUGR impairs mucus barrier development and is associated with long-term alterations of mucin expression. The lack of an efficient colonic barrier induced by IUGR may predispose to colonic injury not only in neonatal life but also in later life.

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“…5b). Tff3 protein is known to be secreted from goblet cells, where it is cotranslationally packaged with mucin (Lin et al, 1999). No immunoreactivity for Tff3 was observed at E13.5 and 15.5.…”

Section: Validation Of Microarray Resultsmentioning

confidence: 97%

Gene expression profile analysis of mouse colon embryonic development

Franklin

Settle

Levy

et al. 2005

genesis

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During late embryogenesis, the mouse colon develops from a pseudostratified, undifferentiated endoderm to a single-layered columnar epithelium with accompanying mesenchymal maturation. To identify regulatory genetic programs underlying these morphological changes, we profiled gene expression of the developing mouse colon by microarray from embryonic day (E)13.5 to E18.5. Unbiased cluster analysis of 13,484 cDNA elements revealed two distinct groups of genes whose expression changes reflect the dynamic morphological events of the epithelium and mesenchyme during this period. Additional analyses revealed two subsets of genes whose expression is either upregulated or downregulated over the same developmental period. Of those genes whose expression increases from E13.5 to E18.5 (n = 158), known functions include acquisition and/or maintenance of colonic differentiation. Genes whose transcription is downregulated over this period (n = 49) have demonstrated roles in nuclear organization, transcriptional regulation, and cell proliferation. These results provide the basis for a molecular portrait of colonic development during late embryogenesis and should be a valuable resource for investigators interested in colonic development and neoplasia, as well as comparative organogenesis.

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Expression of Intestinal Trefoil Factor in Developing Rat Intestine

Cited by 40 publications

References 15 publications

Intestinal Expression of TFF and Related Genes During Postnatal Development in a Piglet Probiotic Trial

Intestinal Expression of TFF and Related Genes During Postnatal Development in a Piglet Probiotic Trial

Intrauterine Growth Restriction Alters Postnatal Colonic Barrier Maturation in Rats

Gene expression profile analysis of mouse colon embryonic development

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