Expression of inflammatory factors and oxidative stress markers in serum of patients with coronary heart disease and correlation with coronary artery calcium score

Abstract

Expression characteristics of inflammatory factors interleukin-23 and interleukin-35; oxidative stress markers of malondialdehyde, which is a final product of lipid peroxidation; superoxide dismutase; microRNA-126 and microRNA-146a in serum of patients with coronary heart disease were investigated. Correlation between these biomarkers and CACS (calcification score), as well as the underlying clinical significance were evaluated. A total of 192 patients diagnosed with coronary heart disease were recruited as the observation group, and 69 healthy adults who provided their blood samples were selected as the control group. Enzyme linked immunosorbent assay was carried out to measure the levels of inflammatory factors interleukin-23 and interleukin-35, and the levels of oxidative stress markers of malondialdehyde and superoxide dismutase in serum of the patients and healthy subjects. Real-time fluorescence-based quantitative PCR was performed to measure the expression levels of microRNA-126 and microRNA-146a in serum. The differences in expression of these biomarkers were analyzed, and correlation between these biomarkers and coronary artery calcium score were assessed. The differences in expression levels of interleukin-23, interleukin-35, malondialdehyde, superoxide dismutase, microRNA-126 and microRNA-146a were statistically significant in both groups. The expression levels of interleukin-23, interleukin-35, malondialdehyde, superoxide dismutase, microRNA-126 and microRNA-146a in the observation group were closely associated with severity of the disease. There were positive correlations between coronary artery calcium score and interleukin-23, interleukin-35, malondialdehyde, microRNA-126 and microRNA-146a, respectively; while a negative correlation existed between coronary artery calcium score and superoxide dismutase in the observation group. In conclusion, biomarkers interleukin-23, interleukin-35, malondialdehyde, superoxide dismutase, microRNA-126 and microRNA-146a were abnormally expressed in serum of patients with coronary heart disease, implicating their association with onset and progression of the disease. The biomarkers were found to be correlated with coronary artery calcium score. Detection of changes of related biomarkers in serum may have certain value in diagnosis of disease formation, as well as assessment of disease severity.

The Profile of Circulating Blood microRNAs in Outpatients with Vulnerable and Stable Atherosclerotic Plaques: Associations with Cardiovascular Risks

“…The mechanism of participation of this miRNA in the process of arterial calcification is poorly understood, but it is believed that it is based on the above-mentioned MAPK/ERK pathway regulation [ 46 ]. The results of our study are consistent with the data of Chen et al, where along with a number of pro-inflammatory markers and miR-146a, a significant increase in the expression of miR126-3p in patients with coronary atherosclerosis and a positive correlation with the level of coronary calcium were found [ 47 ]. In our study, miR-146-5p did not show associations with coronary calcium, and the correlation of this miRNA with a risk of >10% or >7.5% by ACC/AHA is noted at the border of statistical significance ( Table 4 ).…”

Predicting Coupled Herbs for the Treatment of Hypertension Complicated with Coronary Heart Disease in Real-World Data Based on a Complex Network and Machine Learning

“…After an increase in blood pressure, the renin-angiotensin-aldosterone system (RAAS) is overactivated, which induces an inflammatory reaction of target organs such as blood vessels, myocardium, and kidney, resulting in the upregulation of inflammatory cytokines. e coronary artery induces vascular endothelial injury, promotes lipids to enter the intima, causes platelet aggregation, and accelerates the growth of plaques [3,[37][38][39]. To further analyze the therapeutic pathways of coupled herbs, we carried out GO and KEGG enrichment analysis on each herbal target.…”

Genetic and epigenetic modulations in toxicity: The two-sided roles of heavy metals and polycyclic aromatic hydrocarbons from the environment

“…In addition, based on the circulating data in literature, it appears that miRNAs can be useful molecular biomarker in the diagnosis and prediction of diseases. Many of the studies have analyzed the expression levels of some of these miRNAs and evaluated their utility as biomarkers of environmentally mediated diseases [170] , [171] , [172] , [173] , [174] , [175] , [176] , [177] , [178] , [179] . Away from the common view with respect to mechanisms of environmental contaminants such as heavy metals and PAH to involve alteration of redox status, perturbation of inflammation and alteration of mitochondrial function, these environmental chemicals may exert their toxic effects through epigenetic mechanisms such as aberrant expression of miRNAs [155] , [180] , [181] , [182] , [178] .…”