Expression of inflammatory and structural matrix genes in synovial fluid following intra‐articular administration of isoflupredone acetate to exercised horses

Abstract

Expression relative to baseline, for both inflammatory and matrix genes for up to 42 days post IPA administration, suggests a prolonged effect relative to detection time in both plasma and synovial fluid.

A Comprehensive Systematic Review of Intra-Articular Corticosteroids: Symptom and Disease Modification Osteoarthritis Effects and the Potential Impact on Healthy Joints (Part 1)

“…Twenty-one articles were included to answer question 2. These were 10 pre-clinical in vivo studies (Saari et al, 1992;Frisbie et al, 1997;Frisbie et al, 1998;Murray et al, 1998;Hills, Ethell & Hodgson, 1998;Todhunter et al, 1998;Robion et al, 2001;Céleste et al, 2005;Knych et al, 2017;Knych et al, 2018) and 11 in vitro investigations (Murphy et al, 2000;Fubini et al, 2001;Frean, Cambridge & Lees, 2002;Richardson & Dodge, 2003;Doyle et al, 2005;Byron et al, 2008;Busschers, Holt & Richardson, 2010;Palma et al, 2019a;Palma et al, 2019b). Risk of bias was rated for all in vivo studies, which were mainly considered as "unclear risk," with two rated as "high risk" (Figure 3).…”

“…In in vivo studies, seven authors investigated the effects of corticosteroids in normal joints alone (Saari et al, 1992;Murray et al, 1998;Hills, Ethell & Hodgson, 1998;Robion et al, 2001;Céleste et al, 2005;Knych et al, 2017;Knych et al, 2018), while three other included normal joints injected with corticosteroids as one of the groups (Frisbie et al, 1997;Frisbie et al, 1998;Todhunter et al, 1998). These studies considered only results of comparing negative control (normal joints + saline) from corticosteroid groups (injected in normal joints).…”

“…Two studies evaluated Gene expression in SF for TA and IPA (Knych et al, 2017;Knych et al, 2018). MMP-1 and -9 were elevated up to 14 days and 96 h, respectively, while collagen genes were downregulated from 12 h to 42 days after TA IA injection (Knych et al, 2017).…”

Review of glucocorticoid therapy in horses: Intra‐articular corticosteroids

“…Intra-articular (IA) administration is hypothesised to provide a maximal decrease in inflammation, while limiting the considerable systemic effects of corticosteroids (Caron, 2005). The gene-altering effect of corticosteroids may continue beyond the limit of detection of the drug in serum or urine (Knych et al, 2017(Knych et al, , 2018. This is important for both the regulatory control of these drugs (see Regulation of IA corticosteroid therapy in horses) and for optimal clinical use.…”

Clinical insights: Recent developments in equine articular disease (2016–2018)

“…This demonstrated a prolonged alteration in genes related to inflammation and extracellular matrix following TA administration, and this alteration was prolonged in comparison to measured plasma and synovial fluid levels of the drugs . A sister paper from the same group undertook a similar study investigating the effect of another corticosteroid, isoflupredone acetate, on joint metabolism that demonstrated a similar prolonger alteration in gene expression levels in comparison the detection times for the drug .…”