Expression of immunogenic poliovirus Sabin type 1 VP proteins in transgenic tobacco

Bolaños-Martínez, Omayra C.; Govea‐Alonso, Dania O.; Cervantes‐Torres, Jacquelynne; Hernández, Marisela; Fragoso, Gladis; Sciutto-Conde, Edda; Rosales‐Mendoza, Sergio

doi:10.1016/j.jbiotec.2020.07.007

Cited by 11 publications

(13 citation statements)

References 32 publications

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“…This could be ascribed to the possibility that these antigens do not display the immunogenic epitopes in their native conformation [ 59 ] and as subunit vaccine antigens are generally small peptides, they are susceptible to proteolytic degradation which, as a result, also limits the extent of the immune response stimulated [ 60 ]. Progress has been made on the development of several plant-produced subunit vaccines recently, including those against polio [ 61 ], West Nile fever [ 62 , 63 ], rabies [ 64 ], anthrax [ 65 , 66 ] and COVID-19 [ 67 , 68 ], which are all discussed below in more detail.…”

Section: Types Of Plant-produced Vaccinesmentioning

confidence: 99%

“…More recently, poliovirus Sabin subunit capsid-forming proteins (VP1, VP2, VP3 and VP4) were produced individually in transgenic N. tabacum plants. Immunisation of mice with four subcutaneous injections 2 weeks apart and four subsequent oral immunisations with suspensions of lyophilised transgenic leaf material containing all four proteins induced local humoral immune responses, exhibiting potential for further application [ 61 ].…”

Section: Plant-produced Human Vaccinesmentioning

confidence: 99%

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Plant-Derived Human Vaccines: Recent Developments

2022

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The idea of producing vaccines in plants originated in the late 1980s. Initially, it was contemplated that this notion could facilitate the concept of edible vaccines, making them more cost effective and easily accessible. Initial studies on edible vaccines focussed on the use of a variety of different transgenic plant host species for the production of vaccine antigens. However, adequate expression levels of antigens, the difficulties predicted with administration of consistent doses, and regulatory rules required for growth of transgenic plants gave way to the development of vaccine candidates that could be purified and administered parenterally. The field has subsequently advanced with improved expression techniques including a shift from using transgenic to transient expression of antigens, refinement of purification protocols, a deeper understanding of the biological processes and a wealth of evidence of immunogenicity and efficacy of plant-produced vaccine candidates, all contributing to the successful practice of what is now known as biopharming or plant molecular farming. The establishment of this technology has resulted in the development of many different types of vaccine candidates including subunit vaccines and various different types of nanoparticle vaccines targeting a wide variety of bacterial and viral diseases. This has brought further acceptance of plants as a suitable platform for vaccine production and in this review, we discuss the most recent advances in the production of vaccines in plants for human use.

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Section: Types Of Plant-produced Vaccinesmentioning

confidence: 99%

Section: Plant-produced Human Vaccinesmentioning

confidence: 99%

Plant-Derived Human Vaccines: Recent Developments

2022

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“…Also reported similar buoyant density 1.095 g/mL to human serum-derived ones. pHER100 vector transformed cells by ELISA shows 10 ng/mL of spent medium [ 67 ] 22 AgroInfiltration MagnICON viral vectors Transient expression HBsAg assembles into VLPs 295 µg/g leaf FW at post-infection day 10 Polyclonal enzyme-linked immunosorbent assay measures the HBsAg which gets accumulated by the disulphide- linked dimers into VLPs, antigen’s conformational 'a' antigenic determinant was displayed [ 101 , 12 ] 23 Poliomyelitis Agrobacterium- mediated transformation Transient expression Poliovirus capsid proteins- VP1, VP2, VP3, and VP4 Polio antigens 0.31–16.85 µg/g of FW in leaf tissues All candidate transgenic lines express the capsid proteins and dot blot analysis reports positive signal in the hyperimmune sera labelled with Anti-Poliomyelitis Virus 1 antiserum or anti-VP2, anti-VP3, or anti-VP4 under native condition [ 42 ] 24 Measles Agrobacterium -mediated transformation Transient expression Measles virus hemagglutinin (H) protein MAb-366 detected H protein in extracts of pBinH/KDEL 8B (T1) line with greater absorbance than pBin control transgenic Plants converted with the pBinH construct accumulated tiny amounts of H protein, whereas those with the C-terminal KDEL sequence in the pBinH/KDEL construct accumulated more [ 45 ] 25 Dengue Virus Agrobacterium- mediated transformation Transient expression Dengue envelope glycoprotein EIII with cholera toxin B-subunit protein (CTB–EIII) 0.019% TSP Biological activity for intestinal epithelial cell membrane glycolipid receptor was reported in plants with CTB-EIII protein expressed. GM1-ganglioside was evident in GM1-ELISA [ 102 ] 26 Agrobacterium- mediated transformation Transient expression Type 2 Envelope glycoprotein E with immunogenic domain III of the dengue virus (EIII) …”

Section: In Vitro Studiesmentioning

confidence: 99%

Tobacco-Based Vaccines, Hopes, and Concerns: A Systematic Review

Mathew

Thomas

2022

Mol Biotechnol

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Emerging infectious diseases have vigorously devastated the global economy and health sector; cost-effective plant-based vaccines (PBV) can be the potential solution to withstand the current health economic crisis. The prominent role of tobacco as an efficient expression system for PBV has been well-established for decades, through this review we highlight the importance of tobacco-based vaccines (TBV) against evolving infectious diseases in humans. Studies focusing on the use of TBV for human infectious diseases were searched in PubMed, Google Scholar, and science direct from 1995 to 2021 using the keywords Tobacco-based vaccines OR transgenic tobacco OR Nicotiana benthamiana vaccines AND Infectious diseases or communicable diseases. We carried out a critical review of the articles and studies that fulfilled the eligibility criteria and were included in this review. Of 976 studies identified, only 63 studies fulfilling the eligibility criteria were included, which focused on either the in vitro, in vivo, or clinical studies on TBV for human infectious diseases. Around 43 in vitro studies of 23 different infectious pathogens expressed in tobacco-based systems were identified and 23 in vivo analysis studies were recognized to check the immunogenicity of vaccine candidates while only 10 of these were subjected to clinical trials. Viral infectious pathogens were studied more than bacterial pathogens. From our review, it was evident that TBV can be an effective health strategy to combat the emerging viral infectious diseases which are very difficult to manage with the current health facilities. The timely administration of cost-effective TBV can prevent the outburst of viral infections, thereby can protect the global healthcare system to a greater extent.

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“… Bolaños-Martínez et al., 2020 expressed the VP1, VP2, VP3 and VP4 proteins to enable the poliovirus Sabin type 1 capsid formation. The four capsid proteins were expressed in leaves of transgenic tobacco and for each protein the antigenicity was shown.…”

Section: Plant-made Poliovirus Vaccine Candidatesmentioning

confidence: 99%

“…Expression levels ranging from 0.3 µg/g to 16.85 µg/g of fresh leaves were obtained. The immunological potential of the plant-made capsid proteins was further determined by immunizing mice in a scheme comprising subcutaneous and oral boosters with lyophilized plant material containing the four capsid proteins ( Bolaños-Martínez et al., 2020 ). Humoral systemic and mucosal antibody responses were generated with VP1, VP3 and VP4 proteins, while the VP2 protein was less efficient in stimulating specific IgG antibody production.…”

Section: Plant-made Poliovirus Vaccine Candidatesmentioning

confidence: 99%

Plant-made poliovirus vaccines – Safe alternatives for global vaccination

Bolaños-Martínez¹,

Strasser²

2022

Front. Plant Sci.

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Human polioviruses are highly infectious viruses that are spread mainly through the fecal-oral route. Infection of the central nervous system frequently results in irreversible paralysis, a disease called poliomyelitis. Children under five years are mainly affected if they have not acquired immunity through natural infection or via vaccination. Current polio vaccines comprise the injectable inactivated polio vaccine (IPV, also called the Salk vaccine) and the live-attenuated oral polio vaccine (OPV, also called the Sabin vaccine). The main limitations of the IPV are the reduced protection at the intestinal mucosa, the site of virus replication, and the high costs for manufacturing due to use of live viruses. While the OPV is more effective and stimulates mucosal immunity, it is manufactured using live-attenuated strains that can revert into pathogenic viruses resulting in major safety concerns and vaccine-derived outbreaks. During the last fifteen years, plant-based poliovirus vaccines have been explored by several groups as a safe and low-cost alternative, and promising results in protection against challenges with viruses and induction of neutralizing antibodies have been obtained. However, low yields and a high frequency in dose administration highlight the need for improvements in polioviral antigen production. In this review, we provide insights into recent efforts to develop plant-made poliovirus candidates, with an emphasis on strategies to optimize the production of viral antigens.

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Expression of immunogenic poliovirus Sabin type 1 VP proteins in transgenic tobacco

Cited by 11 publications

References 32 publications

Plant-Derived Human Vaccines: Recent Developments

Plant-Derived Human Vaccines: Recent Developments

Tobacco-Based Vaccines, Hopes, and Concerns: A Systematic Review

Plant-made poliovirus vaccines – Safe alternatives for global vaccination

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Expression of immunogenic poliovirus Sabin type 1 VP proteins in transgenic tobacco

Cited by 11 publications

References 32 publications

Plant-Derived Human Vaccines: Recent Developments

Plant-Derived Human Vaccines: Recent Developments

Tobacco-Based Vaccines, Hopes, and Concerns: A Systematic Review

Plant-made poliovirus vaccines – Safe alternatives for global vaccination

Contact Info

Product

Resources

About

Expression of immunogenic poliovirus Sabin type 1 VP proteins in transgenic tobacco