Expression of human lactoferrin in transgenic rice grains for the application in infant formula

Nandi, Somen; Suzuki, Yasushi; Huang, Jianmin; Yalda, Doris; Pham, Phuong; Wu, Liying; Bartley, Glen; Huang, Ning; Lönnerdal, Bo

doi:10.1016/s0168-9452(02)00165-6

Cited by 159 publications

(132 citation statements)

References 31 publications

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“…This finding could have been due to the low concentration of the active peptides in the hydrolysate which could be not enough to affect the viability of S. Enteritidis. This effect has also been observed for enteropathogenic Escherichia coli (EPEC), 35) for which the pepsin or pepsin/pancreatin hydrolysates from human lactoferrin and from the recombinant rice protein exerted lower growth inhibition than the corresponding undigested lactoferrins.…”

Section: Resultsmentioning

confidence: 77%

Antibacterial Activity of Recombinant Human Lactoferrin from Rice: Effect of Heat Treatment

Conesa

Rota

Castillo

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Section: Resultsmentioning

confidence: 77%

Antibacterial Activity of Recombinant Human Lactoferrin from Rice: Effect of Heat Treatment

Conesa

Rota

Castillo

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…A high concentration of lactoferrin is found in human milk and has been suggested to have several biological activities, including protection from pathogens, regulation of iron absorption, modulation of the immune system, and cellular growth promoting activity (Lönnerdal and Iyer 1995). Recombinant lactoferrin has been produced in plants to protect against plant pathogenic bacteria (Mitra and Zhang 1994;Zhang et al 1998) and to increase nutritive value (Nandi et al 2002;Nandi et al 2005). Lfc is the antimicrobial fragment derived from the full length lactoferrin protein upon pepsin cleavage.…”

Section: Discussionmentioning

confidence: 99%

Transgenic tobacco plants expressing antimicrobial peptide bovine lactoferricin show enhanced resistance to phytopathogens

Fukuta

Kawamoto²,

Mizukami

et al. 2012

Plant Biotechnology

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Lactoferricin B, the antimicrobially active N-terminal peptide derived from acid pepsin hydrolysis of bovine lactoferrin, has broad spectrum antimicrobial activities. We expressed the lactoferricin gene in tobacco plants to evaluate its antimicrobial activity. e coding region for the chimeric peptide gene of the signal peptide from tobacco pathogenrelated protein (PR-1) and bovine lactoferricin was synthesized using the recombinant polymerase chain reaction (PCR) method. Transgenic tobacco plants expressing the lactoferricin gene were developed using the Agrobacterium-mediated transformation method. e lactoferricin B gene was integrated into the tobacco genome and its transcription was detected by PCR, Southern blot analysis, and reverse transcription PCR (RT-PCR), respectively. e transgenic tobacco plants were challenged with the pathogenic bacteria Pseudomonas syringae pv. tabaci and Botrytis cinerea. At 30 or 28 days postinoculation, the transgenic plants were still green and continued to grow, whereas the control plants were infected with bacterial or fungal pathogens from the roots to the tips, resulting in death of the plants. In conclusion, transgenic tobacco plants that overexpressed the lactoferricin gene, linked to the signal peptide of tobacco PR-1 protein under the control of a high expression constitutive promoter, showed enhanced resistance to bacterial (P. syringae pv. tabaci) and fungal (B. cinerea) diseases.

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“…For biofortification, transgenic approaches have also been pursued. An example is the endosperm specific expression of the recombinant human lactoferrin (rHLF) gene in rice grain, resulting in production of the HLF protein up to 0.5 % of the grain weight and twofold increase in the grain Fe concentration (Nandi et al 2002).…”

Section: Biofortificationmentioning

confidence: 99%

Zinc – An Indispensable Micronutrient

Sharma

Patni

Shankhdhar

et al. 2012

Physiol Mol Biol Plants

213

124

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Availability of Zn to plant is hampered by its immobile nature and adverse soil conditions. Thus, Zn deficiency is observed even though high amount is available in soil. Root-shoot barrier, a major controller of zinc transport in plant is highly affected by changes in the anatomical structure of conducting tissue and adverse soil conditions like pH, clay content, calcium carbonate content, etc. Zn deficiency results in severe yield losses and in acute cases plant death. Zn deficiency in edible plant parts results in micronutrient malnutrition leading to stunted growth and improper sexual development in humans. To overcome this problem several strategies have been used to enrich Zn availability in edible plant parts, including nutrient management, biotechnological tools, and classical and molecular breeding approaches.

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Expression of human lactoferrin in transgenic rice grains for the application in infant formula

Cited by 159 publications

References 31 publications

Antibacterial Activity of Recombinant Human Lactoferrin from Rice: Effect of Heat Treatment

Antibacterial Activity of Recombinant Human Lactoferrin from Rice: Effect of Heat Treatment

Transgenic tobacco plants expressing antimicrobial peptide bovine lactoferricin show enhanced resistance to phytopathogens

Zinc – An Indispensable Micronutrient

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